Flexible immunosensor for the detection of salivary α-amylase in body fluids

Ventura, Bartolomeo Della; Sakač, Nikola; Funari, Riccardo; Velotta, R.

doi:10.1016/j.talanta.2017.05.075

Cited by 40 publications

(24 citation statements)

References 41 publications

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“…No clear linear portions of the concentrations of B. bifidum can be observed using mouse monoclonal antibody-AuNPs conjugation as detection layer. The results recorded in this study are very competitive with previous reports (Fung and Wong, 2001;Guo et al, 2012;Skládal, 2016;Della Ventura et al, 2017;Wang R. et al, 2017;Gao et al, 2018;Pohanka, 2018aPohanka, ,b, 2019. Table 1 showed the sensing performance comparison.…”

Section: Resultssupporting

confidence: 71%

Rapid Detection of Bifidobacterium bifidum in Feces Sample by Highly Sensitive Quartz Crystal Microbalance Immunosensor

et al. 2020

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In this work, a quartz crystal microbalance (QCM) sensor has been fabricated using immunoassay for sensitive determination of Bifidobacterium bifidum. Au nanoparticle has been used for amplifying sandwich assays. The proposed immunosensor exhibited a linear detection range between 10 3 and 10 5 CFU/mL with a limit of detection of 2.1 × 10 2 CFU/mL. The proposed immunosensor exhibited good selectivity for B. bifidum sensing with low cross reactivity for other foodborne pathogens such as Lactobacillus acidophilus, Listeria monocytogenes, and Escherichia coli. In addition, the proposed immunosensor has been successfully used for B. bifidum detection in feces samples and food samples. The frequency decreases of 12, 17, and 10 Hz were observed from the milk samples consisting of the mixtures of L. acidophilus, L. monocytogenes, and E. coli. The frequency decreases of 8, 15, and 7 Hz were observed from the feces samples consisting of the mixtures of L. acidophilus, L. monocytogenes, and E. coli.

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Section: Resultssupporting

confidence: 71%

Rapid Detection of Bifidobacterium bifidum in Feces Sample by Highly Sensitive Quartz Crystal Microbalance Immunosensor

et al. 2020

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“…Although the free anomeric center is needed for metabolic uptake, and therefore, to amplify the signal, at the same time, these systems are susceptible to enzymatic degradation in the blood by salivary and pancreatic α‐amylases. These enzymes are valuable biomarkers for diagnosing many diseases and monitoring human stress . The structure–activity relationships of various maltodextrins with amylases began as early as the 1950s, and continues today .…”

Section: Resultsmentioning

confidence: 99%

Harnessing the Maltodextrin Transport Mechanism for Targeted Bacterial Imaging: Structural Requirements for Improved in vivo Stability in Tracer Design

et al. 2018

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Diagnosis and localization of bacterial infections remains a significant clinical challenge. Harnessing bacteria-specific metabolic pathways, such as the maltodextrin transport mechanism, may allow specific localization and imaging of small or hidden colonies. This requires that the intrabacterial tracer accumulation provided by the transporter is matched by high serum stability of the tracer molecule. Herein, radiolabeled maltodextrins of varying chain lengths and with free nonreducing/reducing ends are reported and their behavior against starch-degrading enzymes in the blood, which compromise their serum stability, is evaluated. Successful single-photon emission computed tomography (SPECT) imaging is shown in a footpad infection model in vivo by using the newly developed model tracer, [ Tc]MB1143, and the signal is compared with that of F-fluorodeoxyglucose positron emission tomography ([ F]FDG-PET) as a nonbacterial specific marker for inflammation. Although the [ Tc]MB1143 imaging signal is highly specific, it is low, most probably due to insufficient serum stability of the tracer. A series of stability tests with different F-labeled maltodextrins finally yielded clear structural guidelines regarding substitution patterns and chain lengths of maltodextrin-based tracers for nuclear imaging of bacterial infections.

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“…During the last few decades, quartz crystal microbalances (QCMs) have become powerful and well-established noninvasive tools for clinical bioassays, and for investigating biomolecular interactions, due to their high sensitivity, low cost, real time output, and label- or radiation-free entities [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. QCMs are weighting devices based on the piezoelectric properties of the quartz crystal [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction

Maglio

Costanzo

Cercola

et al. 2017

Sensors

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A cost-effective immunosensor for the detection and isolation of dental pulp stem cells (DPSCs) based on a quartz crystal microbalance (QCM) has been developed. The recognition mechanism relies on anti-CD34 antibodies, DPSC-specific monoclonal antibodies that are anchored on the surface of the quartz crystals. Due to its high specificity, real time detection, and low cost, the proposed technology has a promising potential in the field of cell biology, for the simultaneous detection and sorting of stem cells from heterogeneous cell samples. The QCM surface was properly tailored through a biotinylated self-assembled monolayer (SAM). The biotin–avidin interaction was used to immobilize the biotinylated anti-CD34 antibody on the gold-coated quartz crystal. After antibody immobilization, a cellular pellet, with a mixed cell population, was analyzed; the results indicated that the developed QCM immunosensor is highly specific, being able to detect and sort only CD34+ cells. Our study suggests that the proposed technology can detect and efficiently sort any kind of cell from samples with high complexity, being simple, selective, and providing for more convenient and time-saving operations.

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Flexible immunosensor for the detection of salivary α-amylase in body fluids

Cited by 40 publications

References 41 publications

Rapid Detection of Bifidobacterium bifidum in Feces Sample by Highly Sensitive Quartz Crystal Microbalance Immunosensor

Rapid Detection of Bifidobacterium bifidum in Feces Sample by Highly Sensitive Quartz Crystal Microbalance Immunosensor

Harnessing the Maltodextrin Transport Mechanism for Targeted Bacterial Imaging: Structural Requirements for Improved in vivo Stability in Tracer Design

A Quartz Crystal Microbalance Immunosensor for Stem Cell Selection and Extraction

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