Flexuous Rods and Vesicles in Leaf and Petiole Phloem of Little-Cherry Diseased Prunus spp.

Raine, J.; Weintraub, M.; Schroeder, Bea

doi:10.1094/phyto-65-1181

Cited by 26 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Jelkmann!urz.uniheidelberg.de The nucleotide sequence data reported in this paper will appear in the EMBL, GenBank and DDBJ nucleotide sequence databases under the accession number Y10237. characteristic vesicles and elongated virus-like particles were detected by electron microscopy, thus indicating the association of LCD with a clostero-like virus (Raine et al, 1975(Raine et al, , 1979Ragetli et al, 1982). This hypothesis was supported by the extraction of a high molecular mass double-stranded RNA (dsRNA) from LCD-affected sweet cherry (Hamilton et al, 1980 ;Legrand & Verhoyen, 1986 ;Jelkmann, 1995 ;Eastwell & Bernardy, 1996).…”

mentioning

confidence: 72%

Complete genome structure and phylogenetic analysis of little cherry virus, a mealybug-transmissible closterovirus.

Wilhelm¹,

Fechtner

Agranovsky³

1997

Journal of General Virology

View full text Add to dashboard Cite

The 5h-terminal genomic region (8597 nt) of little cherry virus (LChV), a mealybug-borne closterovirus, was cloned from double-stranded RNA, and its sequence determined to complete the 16 934 nt sequence of the monopartite LChV RNA genome. In the 5h to 3h direction, the sequence encompasses ORF 1a, encoding the conserved replicative domains of methyltransferase and helicase, and ORF 1b, encoding RNA polymerase. ORFs 1a and 1b partially overlap (in 0/M 1 configuration), and the LChV replicase is probably expressed by ribosomal frameshifting as a fusion product with a molecular mass of 318 kDa. The N-terminal part of the ORF 1a product contains a papain-like cysteine proteinase (PCP) domain with a predicted cleavage site between Gly-619 and Ser-620. The PCP and the upstream protein domains can be aligned with the equivalent parts of the leader proteins encoded by the whitefly-transmitted lettuce infectious yellows and sweet potato sunken vein closteroviruses. Phylogenetic reconstruction based on the aligned RNA polymerase sequences clearly suggests that the aphid-transmissible and whiteflytransmissible closteroviruses represent two distinct evolutionary lineages, with the mealybug-transmissible LChV being the most remote member of the ' whitefly ' lineage.Little cherry disease (LCD) is distributed worldwide in ornamental and sweet cherry and has a great impact on the fruit quality of infected trees. In infected phloem cells,

show abstract

mentioning

confidence: 72%

Complete genome structure and phylogenetic analysis of little cherry virus, a mealybug-transmissible closterovirus.

Wilhelm¹,

Fechtner

Agranovsky³

1997

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…However, the tonoplast-associated vesicles also show some resemblance to the cytoplasmic vesicles observed near the vacuoles of barley yellow dwarf virus-infected cells (Gill & Chong, 1975) and those observed in leaf cells of Prunus spp. with little-cherry leaf disease (Raine et al, 1975).…”

Section: Cytopathic Structures Associated With Tonoplasts Of Plant Cementioning

confidence: 99%

Cytopathic Structures Associated with Tonoplasts of Plant Cells Infected with Cucumber Mosaic and Tomato Aspermy Viruses

Hatta

Francki

1981

Journal of General Virology

View full text Add to dashboard Cite

“…It has been assumed that LC disease is induced by a virus since the disease was demonstrated to be transmitted by budding or grafting (Foster & Lott, 1947). Subsequently, virus-like particles have been isolated from trees with symptoms of LC disease (Raine, Weintraub & Schroeder, 1975;Ragetli, Elder & Schroeder, 1982). Double-stranded RNA (dsRNA) also has been found in association with LC diseased tissue (Hamilton, Dodds & Raine, 1980;Legrand & Verhoyen, 19856;Eastwell & Bernardy, 1996).…”

Section: Introductionmentioning

confidence: 99%

Comparison between woody indexing and a rapid hybridisation assay for the diagnosis of little cherry disease in cherry trees*

Eastwell

Bernardy

1996

Annals of Applied Biology

View full text Add to dashboard Cite

Sweet cherry (Prunus auium) and sour cherry (Prunus cerasus) trees from orchards in the Kootenay and Okanagan Valleys of British Columbia, Canada were assayed for the presence of little cherry disease by three different methods: Northern blot analysis of double-stranded RNA, woody indexing for fruit symptoms on sweet cherry cv. Lambert, and woody indexing for foliar symptoms on cv. Canindex 1. Results of the three methods were in agreement for 85% of the samples. Of the 78 orchard trees tested, double-stranded RNA isolated from 48 trees hybridised with a radiolabelled cloned probe specific for little cherry disease. When the 48 trees were tested by woody indexing, buds from 41 trees induced fruit symptoms on cv. Lambert, but only 32 yielded foliar symptoms on cv. Canindex 1 under the conditions of the experiment. Of the 30 orchard trees that did not yield a positive response to the Northern blot analysis, 26 samples were negative on cv. Lambert and 26 were negative on cv. Canindex 1. Northern blot analysis of the 78 cv. Lambert indicator trees revealed that there was an absolute correlation between the presence of little cherry disease-associated double-stranded R N A and the development of typical little cherry disease symptoms on the indicator trees. Reliability of woody indexing of orchard samples was impaired by poor transmission of the disease from the inoculating bud to the indicator tree. Woody indexing with cv. Canindex 1 was particularly prone to a large number of apparently erroneous negative results. Of the three protocols used, diagnosis of little cherry disease by Northern blot analysis was found to be the most reliable and offered a greatly accelerated means of diagnosis.

show abstract

Flexuous Rods and Vesicles in Leaf and Petiole Phloem of Little-Cherry Diseased Prunus spp.

Cited by 26 publications

References 0 publications

Complete genome structure and phylogenetic analysis of little cherry virus, a mealybug-transmissible closterovirus.

Complete genome structure and phylogenetic analysis of little cherry virus, a mealybug-transmissible closterovirus.

Cytopathic Structures Associated with Tonoplasts of Plant Cells Infected with Cucumber Mosaic and Tomato Aspermy Viruses

Comparison between woody indexing and a rapid hybridisation assay for the diagnosis of little cherry disease in cherry trees*

Contact Info

Product

Resources

About