Flipping the script: Advances in understanding how and why P4-ATPases flip lipid across membranes

Norris, Adriana C.; Mansueto, Alexander J.; Jimenez, Mariana; Yazlovitskaya, Eugenia M.; Jain, Bhawik K.; Graham, Todd R.

doi:10.1016/j.bbamcr.2024.119700

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2024

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Cited by 7 publications

References 247 publications

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Membrane structure‐responsive lipid scramblase activity of the TMEM63/OSCA family

Miyata,

Nishimura,

Nagata

et al. 2024

FEBS Letters

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Phospholipids are asymmetrically distributed in the plasma membrane (PM), and scramblases disrupt this asymmetry by shuffling phospholipids. We recently identified mouse Tmem63b as a membrane structure‐responsive scramblase. Tmem63b belongs to the TMEM63/OSCA family of ion channels; however, the conservation of the scramblase activity within this family remains unclear. We expressed human TMEM63 paralogs, TMEM63B orthologs, and plant OSCA1.1 in Tmem63b‐deficient mouse pro‐B cells and found that vertebrate TMEM63B orthologs exhibit scramblase activity at the PM. Previously, ten pathogenic human TMEM63B variants were identified, some of which exhibited constitutive scramblase activity. Upon expressing all variants, we found that nine variants displayed constitutive scramblase activity. These results suggest that membrane structure‐responsive scramblase activity at the PM is conserved among vertebrate TMEM63B orthologs.

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Membrane structure‐responsive lipid scramblase activity of the TMEM63/OSCA family

Miyata,

Nishimura,

Nagata

et al. 2024

FEBS Letters

View full text Add to dashboard Cite

show abstract

Measuring Phospholipid Flippase Activity by NBD-Lipid Uptake in Living Yeast Cells

Jain,

Mansueto,

Graham

2024

Methods in Molecular Biology

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Consensus, controversies, and conundrums of P4-ATPases: The emerging face of eukaryotic lipid flippases

Duan,

2024

Journal of Biological Chemistry

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Flipping the script: Advances in understanding how and why P4-ATPases flip lipid across membranes

Cited by 7 publications

References 247 publications

Membrane structure‐responsive lipid scramblase activity of the TMEM63/OSCA family

Membrane structure‐responsive lipid scramblase activity of the TMEM63/OSCA family

Measuring Phospholipid Flippase Activity by NBD-Lipid Uptake in Living Yeast Cells

Consensus, controversies, and conundrums of P4-ATPases: The emerging face of eukaryotic lipid flippases

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