Abstract:Fresh, unfixed meristems of rose (Rosa hybrida L.) were viewed in the scanning electron microscope to determine the morphological differences and organogenesis of flowering and blind shoots. Gluteraldehyde fixed, ethanol dehydrated and critical point dried tissue was severely desiccated with individual cell walls becoming concave. Fresh tissue remained turgid for at least 10 min in the microscope. Visible signs of flower initiation were evidenced by the presence of sepal primordia followed by differentiation o… Show more
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