Flow cytometric assay to assess short-term effects of personal care products on the marine microalga Tetraselmis suecica

Seoane, Marta; Esperanza, Marta; Rioboo, Carmen; Herrero, Concepción; Cid, Ángeles

doi:10.1016/j.chemosphere.2016.12.097

Cited by 53 publications

(20 citation statements)

References 78 publications

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“…The effect of BP3, DHHB and OC was significant at 100 µg/L and above. A similar decreased esterase activity was reported for C. reinhardtii exposed to BP3, although at concentrations in the mg/L range [21,43]. For DHHB, the effect was only observed for the microalgae and for the esterase activity but not for other parameters.…”

Section: Impact On Cell Metabolic Activitysupporting

confidence: 80%

Effect of 10 UV Filters on the Brine Shrimp Artemia salina and the Marine Microalga Tetraselmis sp.

Thorel

Clergeaud

Jaugeon

et al. 2020

Toxics

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The presence of pharmaceutical and personal care product (PPCP) residues in the aquatic environment is an emerging issue due to their uncontrolled release through gray water, and accumulation in the environment that may affect living organisms, ecosystems and public health. The aim of this study is to assess the toxicity of benzophenone-3 (BP-3), bis-ethylhexyloxyphenol methoxyphenyl triazine (BEMT), butyl methoxydibenzoylmethane (BM), methylene bis-benzotriazolyl tetramethylbutylphenol (MBBT), 2-ethylhexyl salicylate (ES), diethylaminohydroxybenzoyl hexyl benzoate (DHHB), diethylhexyl butamido triazone (DBT), ethylhexyl triazone (ET), homosalate (HS) and octocrylene (OC) on marine organisms from two major trophic levels, including autotrophs (Tetraselmis sp.) and heterotrophs (Artemia salina). In general, results showed that both HS and OC were the most toxic UV filters for our tested species, followed by a significant effect of BM on Artemia salina due to BM—but only at high concentrations (1 mg/L). ES, BP3 and DHHB affected the metabolic activity of the microalgae at 100 µg/L. BEMT, DBT, ET, MBBT had no effect on the tested organisms, even at high concentrations (2 mg/L). OC toxicity represents a risk for those species, since concentrations used in this study are 15–90 times greater than those reported in occurrence studies for aquatic environments. For the first time in the literature, we report HS toxicity on a microalgae species at concentrations complementing those found in aquatic environments. These preliminary results could represent a risk in the future if concentrations of OC and HS continue to increase.

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Section: Impact On Cell Metabolic Activitysupporting

confidence: 80%

Effect of 10 UV Filters on the Brine Shrimp Artemia salina and the Marine Microalga Tetraselmis sp.

Thorel

Clergeaud

Jaugeon

et al. 2020

Toxics

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“…Esterases involved in the FDA assay turn over on a time frame of several hours (Jochem, 2000). Therefore, this technique seems appropriate to detect changes in metabolic activity on a day-to-day or even shorter basis, which makes it well suited to monitor short-term phytoplankton responses to environmental changes or to diverse pollutants (Esperanza et al, 2015;Franklin et al, 2001;Prado et al, 2009;Seoane et al, 2017a;Seoane et al, 2017b). Our results showed that, with more subtle measurements, we can detect the impact of MP.…”

Section: Decrease In Esterase Activity As An Early Response To Mp Expmentioning

confidence: 80%

“…Esterase activity in C. neogracile cells was studied using the fluorescein diacetate (FDA) cytometric assay previously described in Seoane et al (2017b). Cells were incubated with FDA at a final concentration of 6 μM for 10 min at room temperature in the dark.…”

Section: Esterase Activitymentioning

confidence: 99%

Polystyrene microbeads modulate the energy metabolism of the marine diatom Chaetoceros neogracile

Seoane

González-Fernández

Soudant

et al. 2019

Environmental Pollution

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Due to the growing concern about the presence of microplastics (MP) in the environment, the number of studies evaluating the toxicity of these small persistent particles on different marine species has increased in recent years. Few studies have addressed their impact on marine phytoplankton, a subject of great concern since they are primary producers of the aquatic food web. The aim of this study is to unravel the cytotoxicity of 2.5 μg mL−1 unlabelled amino-modified polystyrene beads of different sizes (0.5 and 2 μm) on the marine diatom Chaetoceros neogracile. In addition to traditional growth and photosynthesis endpoints, several physiological and biochemical parameters were monitored every 24 h in C. neogracile cells by flow cytometry during their exponential growth (72 h). Dynamic Light Scattering measurements revealed the strong aggregation and the negative charge of the beads assayed in the culture medium, which seemed to minimize particle interaction with cells and potentially associated impacts. Indeed, MP were not attached to the microalgal cell wall, as evidenced by scanning electron micrographs. Cell growth, morphology, photosynthesis, reactive oxygen species levels and membrane potential remained unaltered. However, exposure to MP significantly decreased the cellular esterase activity and the neutral lipid content. Microalgal oil bodies could serve as an energy source for maintaining a healthy cellular status. Thus, MP-exposed cells modulate their energy metabolism to properly acclimate to the stress conditions. Please note that this is an author-produced PDF of an article accepted for publication following peer review. The definitive publisher-authenticated version is available on the publisher Web site.

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“…In addition to cell density measurements, flow cytometry can be used to analyse several morphological parameters, such as relative cell size and complexity and relative fluorescence, which is related to pigment content (Marie et al, 2005;Stachowski-Haberkorn et al, 2013;Mansano et al, 2017). Using fluorescent dyes specific to particular physiological functions, several studies have shown the usefulness of flow cytometry to indicate the toxicity of certain chemicals to microalgae (Prado et al, 2009(Prado et al, , 2012Rioboo et al, 2011;Stachowski-Haberkorn et al, 2013;Seoane et al, 2014Seoane et al, , 2017Esperanza et al, 2015;Dupraz et al, 2016;González-Pleiter et al, 2017). The most commonly investigated physiological functions in these studies are viability, general metabolic activity (often measured by non-specific esterase activity) and reactive oxygen species (ROS), whose accumulation following exposure to certain contaminants can cause oxidative stress and thus cell damage.…”

Section: A C C E P T E Dmentioning

confidence: 99%

Toxicity of binary mixtures of pesticides to the marine microalgae Tisochrysis lutea and Skeletonema marinoi: Substance interactions and physiological impacts

et al. 2019

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This study screened binary mixtures of pesticides for potential synergistic interaction effects on growth of the marine microalgae Tisochrysis lutea and Skeletonema marinoi. It also examined the single and combined effects of three of the most toxic substances on microalgal physiology.Single substances were first tested on each microalgal species to determine their respective EC 50 and concentration-response relationships. The toxicity of six and seven binary mixtures was then evaluated in microplate experiments on the growth of T. lutea and S. marinoi, respectively, using two mixture modelling approaches: isobolograms and the MIXTOX tool, based on Concentration Addition (CA) or Independent Action (IA) models. Significant cases of antagonism (for both species) and synergism (for S. marinoi) were observed for the mixtures of isoproturon and spiroxamine, and isoproturon and metazachlor, respectively.These two mixtures, together with that of isoproturon and diuron, for which additivity was observed, were further studied for their impacts on the physiology of each species. Exposures were thus made in culture flasks at three concentrations, or concentration combinations for mixtures, selected to cause 25%, 50% and 75% growth rate inhibition. The effects of the selected pesticides singly and in combination were evaluated at three perceived effect concentrations on esterase metabolic activity, relative lipid content, cytoplasmic membrane potential and reactive oxygen species (ROS) content by flow cytometry, and on photosynthetic quantum yield (ϕ' M ) by PAM-fluorescence.Isoproturon and diuron singly and in mixtures induced 20-40% decreases in ϕ' M which was in turn responsible for a significant decrease in relative lipid content for both species. Spiroxamine and metazachlor were individually responsible for an increase in relative lipid content (up to nearly 300% for Highlights ► The toxicity of binary pesticide mixtures was evaluated on two microalgal species. ► Effects of three selected mixtures were then examined on five physiological functions. ► Effects on microalgal physiology were linked to the pesticide's toxic mode of action. ► Mixed isoproturon and metazachlor had significant synergistic effects on S. marinoi. ► Observed synergy could be due to a combined effect on the photosynthetic apparatus.

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Flow cytometric assay to assess short-term effects of personal care products on the marine microalga Tetraselmis suecica

Cited by 53 publications

References 78 publications

Effect of 10 UV Filters on the Brine Shrimp Artemia salina and the Marine Microalga Tetraselmis sp.

Effect of 10 UV Filters on the Brine Shrimp Artemia salina and the Marine Microalga Tetraselmis sp.

Polystyrene microbeads modulate the energy metabolism of the marine diatom Chaetoceros neogracile

Toxicity of binary mixtures of pesticides to the marine microalgae Tisochrysis lutea and Skeletonema marinoi: Substance interactions and physiological impacts

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