Flow cytometric immunophenotypic profiles of mature gamma delta T‐cell malignancies involving peripheral blood and bone marrow

Abstract

Background: In this study we compared clinical findings with flow cytometric immunophenotypic results in a series of patients with aggressive and indolent gamma delta T-cell malignancies with peripheral blood and/or bone marrow involvement.Methods: Gamma delta T-cell malignancies were detected based on flow cytometric demonstration of an abnormal T-cell population staining positive with T-cell receptor gamma delta and confirmed by morphologic and clinical reviews. Clinical data were obtained through chart review and discussion with the patients' physicians.Results: Blood or bone marrow involvement was present in all patients. Hepatosplenic and cutaneous gamma delta T-cell lymphomas had an aggressive clinical course, whereas the gamma delta T-cell large granular lymphocyte (LGL) leukemias had an indolent course. Expressions of CD5, CD8, CD16, and CD57 differed in gamma delta T-cell LGL leukemia compared with hepatosplenic and cutaneous gamma delta T-cell lymphomas.Conclusions: Gamma delta T-cell malignancies have a poor prognosis with the exception of gamma delta T-cell LGL leukemia (indolent process). Because CD57 expression is specific for gamma delta T-cell LGL leukemias, expression of this antigen may be associated with a more indolent clinical course. Because cutaneous gamma delta T-cell lymphoma can present with peripheral blood involvement, flow cytometric evaluation of peripheral blood is important in staging these patients. Published 2005 Wiley-Liss, Inc.

FCRL1 on chronic lymphocytic leukemia, hairy cell leukemia, and B-cell non-Hodgkin lymphoma as a target of immunotoxins

“…25,26 The antibody panels were chosen based on the number of cells, previous histologic diagnosis, and available clinical history. The presence of tumor cells was determined using an extensive panel of antibodies and confirmed by detection of light chain restriction in cells with the appropriate tumor-specific immunophenotype.…”

“…Four-color cytometry was performed with a FACSCalibur flow cytometer (BD Biosciences) as described. 25 …”

TCRγδ+ large granular lymphocyte leukemias reflect the spectrum of normal antigen-selected TCRγδ+ T-cells

“…þ T-Lgl leukemias form a rare subgroup of mature T-cell malignancies, representing approximately 5% of CD3 þ T-LGL LGL leukemia, 7,20 but data on large series are lacking. Here we describe the clinical presentation and laboratory parameters of an unprecedentedly large group of newly diagnosed patients with TCRgd þ T-LGL leukemia.…”

“…True cases of TCRgd þ T-LGL leukemia have only been described anecdotally. [15][16][17][18][19][20][21][22] We therefore aimed to assess the clinical features, immunophenotypical characteristics, and TCR gene rearrangement pattern of a large series of 44 TCRgd þ T-LGL leukemias in order to get more insight into the molecular pathogenesis of this disease. A major part of the cases showed Vg9/Vd2 expression with the typical antigen-selected characteristics as seen in antigen-selected TCRgd þ T lymphocytes of older children and adults.…”

Elevation of soluble CD307 (IRTA2/FcRH5) protein in the blood and expression on malignant cells of patients with multiple myeloma, chronic lymphocytic leukemia, and mantle cell lymphoma

“…The cells were stained with a panel of antibodies as previously described. 19 In specimens from patients with MM, aliquots of cells were permeabilized post cell surface staining and before staining with anti-kappa and anti-lambda goat polyclonal antibody reagents (BD biosciences, San Jose, CA, USA) using the Fix and Perm Cell Permeabilization kit (Caltag Laboratories, Burlingame, CA, USA) according to manufacturer's specifications. The antibody panels were chosen based upon the number of cells, previous histological diagnosis and available clinical history.…”