Flow Cytometric Quantification, Isolation, and Subsequent Epigenetic Analysis of Tetraploid Neurons

López-Sánchez, Noelia; Patiño-Parrado, Iris; Frade, José Marı́a

doi:10.1007/978-1-4939-7280-7_4

Cited by 2 publications

(1 citation statement)

References 78 publications

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“…Samples are then immediately run live on a flow cytometer for analysis. We employed strict gating parameters to eliminate doublets ( Figure 1—figure supplement 1 ; López-Sánchez et al, 2017b ). This assay is sensitive enough to measure DNA content from small subsets of cells (e.g.…”

Section: Resultsmentioning

confidence: 99%

Polyploidy in the adult Drosophila brain

Nandakumar

Grushko

Buttitta

2020

eLife

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Long-lived cells such as terminally differentiated postmitotic neurons and glia must cope with the accumulation of damage over the course of an animal’s lifespan. How long-lived cells deal with ageing-related damage is poorly understood. Here we show that polyploid cells accumulate in the adult fly brain and that polyploidy protects against DNA damage-induced cell death. Multiple types of neurons and glia that are diploid at eclosion, become polyploid in the adult Drosophila brain. The optic lobes exhibit the highest levels of polyploidy, associated with an elevated DNA damage response in this brain region. Inducing oxidative stress or exogenous DNA damage leads to an earlier onset of polyploidy, and polyploid cells in the adult brain are more resistant to DNA damage-induced cell death than diploid cells. Our results suggest polyploidy may serve a protective role for neurons and glia in adult Drosophila melanogaster brains.

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Section: Resultsmentioning

confidence: 99%

Polyploidy in the adult Drosophila brain

Nandakumar

Grushko

Buttitta

2020

eLife

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Polyploidy in the adultDrosophilabrain

Nandakumar

Grushko

Buttitta

2019

Preprint

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Long-lived cells such as terminally differentiated postmitotic neurons and glia must cope with the accumulation of damage over the course of an animal's lifespan. How long-lived cells deal with ageing-related damage is poorly understood. Here we show that polyploid cells accumulate in the ageing adult fly brain and that polyploidy protects against DNA damage-induced cell death. Multiple types of neurons and glia that are diploid at eclosion, become polyploid with age in the adult Drosophila brain. The optic lobes exhibit the highest levels of polyploidy, associated with an elevated DNA damage response in this brain region with age. Inducing oxidative stress or exogenous DNA damage leads to an earlier onset of polyploidy, and polyploid cells in the adult brain are more resistant to DNA damage-induced cell death than diploid cells. Our results suggest polyploidy may serve a protective role for neurons and glia in ageing Drosophila melanogaster brains. for providing fly stocks, particularly the labs of Cheng-

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Flow Cytometric Quantification, Isolation, and Subsequent Epigenetic Analysis of Tetraploid Neurons

Cited by 2 publications

References 78 publications

Polyploidy in the adult Drosophila brain

Polyploidy in the adult Drosophila brain

Polyploidy in the adultDrosophilabrain

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