Flow cytometric S-phase fraction contributes to diagnosis of diploid malignant salivary gland tumours

Driemel, Oliver; Kraft, K.; Hemmer, Joerg

doi:10.1016/j.ijom.2006.03.025

Cited by 5 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A vast majority of studies have used FCM to evaluate DNA content in salivary gland tumours, and the samples have been obtained from fresh tumour tissue or paraffin blocks (10–13, 18, 22–34). Takashima et al (13) suggested that a combination of magnetic resonance, cytology and FCM is optimal for diagnosing malignancies of parotid gland, and fine needle aspiration biopsy (FNAB) derived materials can replace the surgical specimens in FCM analysis.…”

Section: Discussionmentioning

confidence: 99%

DNA ploidy analysis in salivary gland tumours by image cytometry

Vargas

Torres-Rendon

Speight

2007

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

DNA ploidy analysis in salivary gland tumours by image cytometry

Vargas

Torres-Rendon

Speight

2007

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

“…However, the frequency of aneuploidy in this group of lesions is lower than in other solid tumors (9). In addition, the molecular basis of chromosomal instability and aneuploidy in salivary neoplasms is not clear.…”

Section: Introductionmentioning

confidence: 75%

DNA Aneuploidy in Malignant Salivary Gland Neoplasms is Independent of USP44 Protein Expression

et al. 2017

View full text Add to dashboard Cite

Chromosomal instability, leading to aneuploidy, is one of the hallmarks of human cancers. USP44 (ubiquitin specific peptidase 44) is an important molecule that plays a regulatory role in the mitotic checkpoint and USP44 loss causes chromosome missegregation, aneuploidy and tumorigenesis in vivo. In this study, it was investigated the immunoexpression of USP44 in 28 malignant salivary gland neoplasms and associated the results with DNA ploidy status assessed by image cytometry. USP44 protein was widely expressed in most of the tumor samples and no clear association could be established between its expression and DNA ploidy status or tumor size. On this basis, it may be concluded that the aneuploidy of the salivary gland cancers included in this study was not driven by loss of USP44 protein expression.

show abstract

“…The S-phase fraction in a DNA histogram reflects the number of cells in an aneuploid status, and has been deemed to be representative of a lesion's proliferative activity and suggestive of its genetic stability [41]. S-phase measurement has been widely used in patients with different carcinomas [41][42][43] and is a clinically useful prognostic factor in patients with OSCC [43][44][45][46][47][48].…”

Section: Discussionmentioning

confidence: 99%

“…S-phase measurement has been widely used in patients with different carcinomas [41][42][43] and is a clinically useful prognostic factor in patients with OSCC [43][44][45][46][47][48]. Additionally, the percentage of aneuploid cells in S-phase, unlike aneuploidy as a discrete biomarker, represents a continuous variable that allows the application of powerful statistical approaches like linear regression models to disclose a relationship with other continuous variables, such as the number of chromosomal alterations in the present study.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Cytogenetic and Static Cytometry Procedures in the Evaluation of Potentially Malignant Oral Lesions

Montebugnoli

Grelli

Cervellati

et al. 2008

Scholarly Research Exchange

View full text Add to dashboard Cite

Gross genomic damage or specific chromosomal alterations have been revealed by different laboratory procedures in potentially malignant oral lesions, but two or more procedures have never been applied at the same time to the same cell population. In the present study we considered cell suspensions obtained from 34 oral lesions at risk of malignancy to see whether they might harbour genetic alterations and whether a correlation exists between the results obtained by two different methods of assessing DNA aberrations. Each suspension underwent DNA-content assessment by static cytometry, and cytogenetic G-banding analysis of short-term primary cultures. DNA content was determined in a minimum of 1000 cells on a Fairfield ploidy analyser and results expressed as percent of aneuploid cells in the S-phase; cytogenetic analysis was carried out according to standard procedures on in situ G-banding metaphases, and results expressed as percent of metaphases with chromosomal alterations. The results showed that the percentage of metaphases with chromosomal alterations was significantly correlated with the percentage of aneuploid cells in the S-phase. In conclusion, genetic alterations can be revealed in the same oral specimen either by procedures studying DNA content in fixed cells or by procedures investigating chromosomal alterations in cultured and proliferating cells.

show abstract

Flow cytometric S-phase fraction contributes to diagnosis of diploid malignant salivary gland tumours

Cited by 5 publications

References 16 publications

DNA ploidy analysis in salivary gland tumours by image cytometry

DNA ploidy analysis in salivary gland tumours by image cytometry

DNA Aneuploidy in Malignant Salivary Gland Neoplasms is Independent of USP44 Protein Expression

Comparison of Cytogenetic and Static Cytometry Procedures in the Evaluation of Potentially Malignant Oral Lesions

Contact Info

Product

Resources

About