Flow Cytometrically Determined DNA Content of Breast Carcinoma and Benign Lesions: Correlations with Histopathological Parameters

Uccelli, Raffaella; Calugi, A; Forte, D.; Mauro, F.; Polonio-Balbi, P; Vecchione, Andrea; Vizzone, A; R, De Vita

doi:10.1177/030089168607200209

Cited by 12 publications

(4 citation statements)

References 21 publications

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“…Several other studies reveal a close correlation between aneuploid tumors and increasing histologic grade. 6,8,20,28,36 Other studies revealed no significant correlation between DNA content and histologic grade. 1,3,26 Previous studies on stage of breast carcinoma have yielded conflicting results.…”

Section: Discussionmentioning

confidence: 94%

Is DNA ploidy an independent prognostic indicator in infiltrative node-negative breast adenocarcinoma?

Keyhani‐Rofagha¹,

O’Toole²,

Farrar³

et al. 1990

Cancer

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To evaluate DNA content as an independent, long-term prognostic indicator in infiltrative, node-negative adenocarcinoma of the breast, flow cytometric DNA analyses were performed retrospectively in 165 patients. The exclusive use of paraffin-embedded tissue permitted the study of patients for whom 3 to 15 years' follow-up was available. Other investigators have shown a relationship between DNA nuclear content and well-known prognostic indicators of breast carcinoma, such as estrogen receptors, age, menopausal status, and stage of tumor. However, very few of these studies were based on patients with long-term clinical follow-up and investigated the independent prognostic value of DNA nuclear content. The frequency of aneuploidy in the patients in this study was 57%, with DNA indices ranging from 0.73 to 2.59. Survival in patients with localized breast carcinoma with DNA aneuploid tumors was 84.1% at 5 years and 75.5% at 10 years. Those with diploid tumors showed survival of 87.8% at 5 years and 73.4% at 10 years. These data show no independent prognostic value for DNA nuclear content. The relationship between DNA content, tumor histologic type, and age was also investigated. High-grade tumors were more often aneuploid. There was no association between DNA content and age. Of 165 patients, information regarding estrogen receptor status was available in only 77; 45% of those with aneuploid tumors and 35% with diploid tumors were estrogen receptor-negative. This difference was not significant. The authors conclude that simple determination of DNA ploidy fails to indicate prognosis for infiltrative, node-negative breast carcinoma.

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Section: Discussionmentioning

confidence: 94%

Is DNA ploidy an independent prognostic indicator in infiltrative node-negative breast adenocarcinoma?

Keyhani‐Rofagha¹,

O’Toole²,

Farrar³

et al. 1990

Cancer

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“…These results would fall midway within the, albeit large, range of values reported by others (Kute et al, 1981;Olszewski et al, 1981) (Dixon et al, 1984), it is more widely employed for routine diagnosis. In certain cases, flow cytometric DNA analysis of FNA material may provide additional diagnostic information in that although DNA aneuploid cells have been detected in benign lesions of the breast (Cornelisse et al, 1983;Uccelli et al, 1986) and other tissues (Danova et al, 1987;Jarvis et al, 1987;Joensuu et al, 1986), the incidence is relatively low. The presence of a distinct population of DNA aneuploid cells may therefore be indicitive of malignancy.…”

Section: Resultsmentioning

confidence: 99%

DNA analysis of breast tumour fine needle aspirates using flow cytometry

Levack

Mullen²,

Anderson³

et al. 1987

Br J Cancer

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Summary Cellular DNA was analysed by flow cytometry in fine needle aspirates (FNA) from both benign and malignant breast lesions in order to determine the feasibility of flow cytometric analysis. In 22 of 26 (84%) benign and 69 of 74 (93%) malignant aspirates, sufficient cells were present to produce good quality DNA histograms. DNA in all 22 benign lesions was diploid. In contrast, of the 69 cancers with sufficient cells for analysis, 40 The method of aspiration has been described previously (Zajicek, 1965), but briefly aspirates were obtained using a 23 gauge needle attached to a 10ml syringe. The lesion was localised with the needle tip and negative pressure applied. Using a gentle pumping action, material was aspirated into the needle, the pressure was released, and the needle withdrawn.Material was expelled onto 4 dry glass slides and smears prepared: 2 were air dried for subsequent May Grunwald Giemsa staining, and 2 were fixed in alcohol for Papanicoulaou staining. All slides were examined by two pathologists and categorised as either malignant, suspicious, benign or acellular (Dixon et al., 1984). A further drop of aspirated material was taken for immunohistochemical assay of the oestrogen receptor. The remainder of the aspirate, which was to be used for flow cytometric analysis, was expelled into 200Il of citrated buffer, rapidly frozen on dry ice, and stored at -40°C until analysed (Vindel0v et al., 1983a

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“…Most of them have dealt with morphological aspects [12] and DNA content [5][6][7][8][9][10][11][12], and only a few with the rate of cell proliferation [4]. In addition, only a few studies [7,9] have compared DNA content defined on FNAs or core needle biopsies at the time of diagnosis to that detected on the correspondent whole tumor specimen at the time of surgery for patients who had not been given any intercurrent therapy.…”

Section: Introductionmentioning

confidence: 99%

Comparing core needle to surgical biopsies in breast cancer for cell kinetic and ploidy studies

Daidone

Orefice

Mastore

et al. 1991

Breast Cancer Res Tr

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The evaluability and reliability of proliferative activity (expressed as 3H-thymidine labeling index, 3H-TdR LI) and ploidy determinations on core needle biopsies were compared with those obtained on surgical material from the same breast cancers. The evaluability of 3H-TdR LI on core needle biopsies was markedly lower than that on surgical material (53% vs 100%), and the association between 3H-TdR LI values in the 16 cases with both evaluable determinations was poor (rs = 0.45). Conversely, determinations of ploidy on core needle biopsy and surgical material provided superimposable results, in terms of evaluability (91% vs 100%) and reliability (rs = 0.99). Further efforts are needed to improve sampling procedures for a reliable assessment of biological markers.

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Flow Cytometrically Determined DNA Content of Breast Carcinoma and Benign Lesions: Correlations with Histopathological Parameters

Cited by 12 publications

References 21 publications

Is DNA ploidy an independent prognostic indicator in infiltrative node-negative breast adenocarcinoma?

Is DNA ploidy an independent prognostic indicator in infiltrative node-negative breast adenocarcinoma?

DNA analysis of breast tumour fine needle aspirates using flow cytometry

Comparing core needle to surgical biopsies in breast cancer for cell kinetic and ploidy studies

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