Flow cytometry and automatic blood cell analysis in striped bass Morone saxatilis (Walbaum, 1792): A new hematological approach

Fazio, Francesco; Saoca, Concetta; Costa, Guilherme; Zumbo, Alessandro; Piccione, Giuseppe; Parrino, Vincenzo

doi:10.1016/j.aquaculture.2019.734398

Cited by 47 publications

(31 citation statements)

References 27 publications

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“…One favorable option for rapid and non-lethal sampling of large numbers of fish individuals is blood analysis. Blood is a complex mixture of heterogeneous cell populations (6)(7)(8) that include erythrocytes (red blood cells), leukocytes (white blood cells), and thrombocytes (analogs of mammalian platelets) (9). These fish blood cells are broadly similar in function to their mammalian counterparts and are found in all other tissues and organs throughout the body.…”

Section: Introductionmentioning

confidence: 99%

Blood Will Tell: What Hematological Analyses Can Reveal About Fish Welfare

2021

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Blood analyses provide substantial information about the physiological aspects of animal welfare assessment, including the activation status of the neuroendocrine and immune system, acute and long-term impacts due to adverse husbandry conditions, potential diseases, and genetic predispositions. However, fish blood is still not routinely analyzed in research or aquaculture for the assessment of health and/or welfare. Over the years, the investigative techniques have evolved from antibody-based or PCR-based single-parameter analyses to now include transcriptomic, metabolomic, and proteomic approaches and from hematological observations to fluorescence-activated blood cell sorting in high-throughput modes. The range of testing techniques established for blood is now broader than for any other biogenic test material. Evaluation of the particular characteristics of fish blood, such as its cell composition, the nucleation of distinct blood cells, or the multiple isoforms of certain immune factors, requires adapted protocols and careful attention to the experimental designs and interpretation of the data. Analyses of fish blood can provide an integrated picture of the endocrine, immunological, reproductive, and genetic functions under defined environmental conditions and treatments. Therefore, the scarcity of high-throughput approaches using fish blood as a test material for fish physiology studies is surprising. This review summarizes the wide range of techniques that allow monitoring of informative fish blood parameters that are modulated by different stressors, conditions, and/or treatments. We provide a compact overview of several simple plasma tests and of multiparametric analyses of fish blood, and we discuss their potential use in the assessment of fish welfare and pathologies.

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Section: Introductionmentioning

confidence: 99%

Blood Will Tell: What Hematological Analyses Can Reveal About Fish Welfare

2021

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“…Generally, fish hematological and serum biochemical indices are considered vital measurements and biological indicators for evaluating the general health status, hepatorenal functions [ 87 , 88 ], and physiological stress responses of fish fed formulated diets [ 89 , 90 ].…”

Section: Hematobiochemical Parametersmentioning

confidence: 99%

The Feasibility of Using Yellow Mealworms (Tenebrio molitor): Towards a Sustainable Aquafeed Industry

Shafique

Abdel‐Latif

Hassan

et al. 2021

Animals

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The success of the aquafeed industry mainly depends on the availability of raw ingredients with high nutritional value, such as fishmeal (FM). However, the increased demand for FM elevates its prices and leads to high feed costs. Thus, there is an urgent need to find suitable alternatives for FM in fish diets to achieve sustainability in aquaculture. Currently, attention is being paid to the possibility of using insect meals as FM substitutes in aquafeed because of their relatively high nutritional quality. TM is one of those insects that can be regarded as a unique candidate because of its relatively high nutritional value. TM are rich sources of essential amino acids (methionine), lipids, and fatty acids, which vary based on the developmental stage of the worms. Although TM have an abundant amount of chitin as a fiber source and other anti-nutritional factors, numerous studies have investigated the efficacy of partial or complete substitution of FM by T. molitor in fish diets. In this context, we reviewed the current research findings on the achievable inclusion levels of T. molitor versus FM substitution in the diets of several finfish and shellfish species. We discussed the potential use of T. molitor as an FM substitute in fish diets and evaluated its effects on growth, biometric indices, and body composition. Besides, the hematological parameters, immunological responses, antioxidative efficacy, intestinal health status, and sensory criteria of fish fed T. molitor-based diets were also assessed.

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“…With multiple advantages of noninvasion, nondestruction, universal response, high throughput, and good resolution, NMR shows potent metabolic profiling capabilities and has been widely used for metabolomic analysis [10][11][12]. A recent study shows the role of metabolomics in order to assess the effects of environmental pollution and metabolomics research in the health and safety of marine organisms [13,14]. At present, there are related researches on aquaculture marine foods.…”

Section: Introductionmentioning

confidence: 99%

Discriminant Analysis of Traditional Chinese Medicinal Properties Based on Holistic Chemical Profiling by ¹H‐NMR Spectrometry

Zhang

Guo

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Medicinal property, which is closely related to drug chemical profiling, is the essence of traditional Chinese medicine (TCM) theory and has always been the focus of modern Chinese medicine. Based on dozens of classic and commonly used TCM herbs with recognized medicinal properties, the present study just aimed to investigate the feasibility and reliability of medicinal property discriminant by using 1H-NMR spectrometry, which provided a mass of spectral data showing holistic chemical profile for multivariate analysis and data mining, including principal component analysis (PCA), Fisher linear discriminant analysis (FLDA), and canonical discriminant analysis (CDA). By using FLDA for two-class recognition, a large majority of test herbs (59/61) were properly discriminated as cold or hot group, and the only two exceptions were Chuanbeimu (Fritillariae Cirrhosae Bulbus) and Rougui (Cinnamomi Cortex), suggesting that medicinal properties interrelate with flavor and body tropism, and all these factors together bring up medicinal property and efficacy. While by performing CDA, 98.4% of the original grouped herbs and 77.0% of the leave-one-out cross-validated grouped cases were correctly classified. The findings demonstrated that discriminant analysis based on holistic chemical profiling data by 1H-NMR spectrometry may provide a powerful alternative to have a deeper understanding of TCM medicinal property.

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Flow cytometry and automatic blood cell analysis in striped bass Morone saxatilis (Walbaum, 1792): A new hematological approach

Cited by 47 publications

References 27 publications

Blood Will Tell: What Hematological Analyses Can Reveal About Fish Welfare

Blood Will Tell: What Hematological Analyses Can Reveal About Fish Welfare

The Feasibility of Using Yellow Mealworms (Tenebrio molitor): Towards a Sustainable Aquafeed Industry

Discriminant Analysis of Traditional Chinese Medicinal Properties Based on Holistic Chemical Profiling by ¹H‐NMR Spectrometry

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Flow cytometry and automatic blood cell analysis in striped bass Morone saxatilis (Walbaum, 1792): A new hematological approach

Cited by 47 publications

References 27 publications

Blood Will Tell: What Hematological Analyses Can Reveal About Fish Welfare

Blood Will Tell: What Hematological Analyses Can Reveal About Fish Welfare

The Feasibility of Using Yellow Mealworms (Tenebrio molitor): Towards a Sustainable Aquafeed Industry

Discriminant Analysis of Traditional Chinese Medicinal Properties Based on Holistic Chemical Profiling by 1H‐NMR Spectrometry

Contact Info

Product

Resources

About

Discriminant Analysis of Traditional Chinese Medicinal Properties Based on Holistic Chemical Profiling by ¹H‐NMR Spectrometry