Flow cytometry description of a novel CD3?/CD7+ intraepithelial lymphocyte subset in human duodenal biopsies: Potential diagnostic value in coeliac disease

Identification of CD7 as a Cognate of the Human K12 (SECTM1) Protein

“…CD7 is expressed on mature T and NK cells, as well as on progenitors of T, B, NK, and myeloid cells (9,10,14,15) and on intestinal intraepithelial lymphocytes (21,22). CD7 is thought to be a marker for one of the earliest stages of T cell development (reviewed in Refs.…”

Autologous hematopoietic stem cell transplantation in refractory celiac disease with aberrant T cells

“…24,25 T-cell receptor (TCR) gene rearrangement study, [12][13][14] T-cell flow cytometry, and IEL phenotyping were performed. 15,26,27 Laboratory evaluation included whole blood cell counts and serum levels of creatinine, bilirubin, liver enzymes, lactate dehydrogenase, albumin, electrolytes, iron, ferritin, folic acid, and vitamin B 12 were determined. EMA and anti-tTG assays, HLA-DQ typing, thyroid function tests, stool examination for Giardia and other parasites, and HIV serology were also performed.…”

“…Aberrant T cells were defined either as CD7 ϩ surface CD3 Ϫ cells (expressed as percent of CD103 ϩ lymphocytes) or as cytoplasmic CD3 ϩ , surface CD3 Ϫ cells (expressed as percent of CD103 ϩ lymphocytes). 12,26 All flow cytometry analyses were performed by an analyst and interpreted by the same medical immunologist; histopathology was performed by the same pathologist to ensure uniformity, reproducibility, and consistency of results.…”

Isolation of human small bowel intraepithelial lymphocytes by Annexin V-coated magnetic beads

“…13 The majority of CD3 þ IEL are CD8 þ Tc. 14 The presence of CD19 þ Bc (42%) or a high proportion of CD4 þ Tc (415%) in the IEL preparation after DTT/EDTA treatment is suggestive of contamination with lamina propria lymphocytes, and such cases were not included in our study. Figure 3a illustrates the relatively low proportions of CD103 À , CD19 þ and CD4 þ cells in a representative sample prior to magnetic separation.…”