Flow induced HeLa cell detachment kinetics show that oxygen-containing functional groups in graphene oxide are potent cell adhesion enhancers

Vlček, Jakub; Lapčík, Ľubomír; Havrdová, Markéta; Poláková, Kateřina; Lapčı́ková, Barbora; Opletal, Tomáš; Froning, Jens P.; Otyepka, Michal

doi:10.1039/c8nr08994a

Cited by 18 publications

(15 citation statements)

References 48 publications

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“…The vinculin density was similar for PT and PT/P group. The higher vinculin density of 0.025PTG/P, 0.05PTG/P and 0.1PTG/P groups compared to PT and PT/P group was because that the oxygen-containing group and topographical features of GO contained scaffolds could greatly enhance the initial cell adhesion [37,38]. In addition, the higher wettability of these GO contained scaffolds could also explain the higher vinculin density of rMSCs on them [39].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the higher wettability of these GO contained scaffolds could also explain the higher vinculin density of rMSCs on them [39]. Moreover, GO was reported to adsorb serum protein and pro-concentrate osteogenic inducers, such as dexamethasone, β-glycerophosphate, and ascorbic acid, through the interaction between the π–π electron cloud in GO and the aromatic rings in the biomolecules, which is also beneficial for osteogenic differentiation [34,35,36,37,38,39,40]. Therefore, the increase in ALP activity level in the 0.05PTG/P and 0.1PTG/P groups on day 14 may be the result of the sustained release of the peptide and the intrinsic properties of GO.…”

Section: Discussionmentioning

confidence: 99%

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Fabrication and Application of Novel Porous Scaffold in Situ-Loaded Graphene Oxide and Osteogenic Peptide by Cryogenic 3D Printing for Repairing Critical-Sized Bone Defect

Zhang

Wang

et al. 2019

Molecules

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Osteogenic peptides have been reported as highly effective in directing mesenchymal stem cell osteogenic differentiation in vitro and bone formation in vivo. Therefore, developing novel biomaterials for the controlled delivery of osteogenic peptides in scaffolds without lowering the peptide’s biological activity is highly desirable. To repair a critical-sized bone defect to efficiently achieve personalized bone regeneration, a novel bioactive poly(lactic-co-glycolic acid) (PLGA)/β-tricalcium phosphate (β-TCP) composite scaffold, in which graphene oxide (GO) and bone morphogenetic protein (BMP)-2-like peptide were loaded in situ (PTG/P), was produced by an original cryogenic 3D printing method. The scaffolds were mechanically comparable to human cancellous bone and hierarchically porous. The incorporation of GO further improved the scaffold wettability and mechanical strength. The in situ loaded peptides retained a high level of biological activity for an extended time, and the loading of GO in the scaffold further tuned the peptide release so that it was more sustained. Our in vitro study showed that the PTG/P scaffold promoted rat bone marrow-derived mesenchymal stem cell ingrowth into the scaffold and enhanced osteogenic differentiation. Moreover, the in vivo study indicated that the novel PTG/P scaffold with sustained delivery of the peptide could significantly promote bone regeneration in a critical bone defect. Thus, the novel bioactive PTG/P scaffold with a customized shape, improved mechanical strength, sustainable peptide delivery, and excellent osteogenic ability has great potential in bone tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fabrication and Application of Novel Porous Scaffold in Situ-Loaded Graphene Oxide and Osteogenic Peptide by Cryogenic 3D Printing for Repairing Critical-Sized Bone Defect

Zhang

Wang

et al. 2019

Molecules

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“…The values of these parameters were obtained from the literature, and the results are summarized in Table 2 . [ 44,49 ]…”

Section: Resultsmentioning

confidence: 99%

Influence of Graphene Oxide on Thermally Induced Shape Memory Behavior of PLA/TPU Blends: Correlation with Morphology, Creep Behavior, Crystallinity, and Dynamic Mechanical Properties

Azadi

Jafari

Khonakdar

et al. 2020

Macro Materials & Eng

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In this study, shape memory is thermally induced in a series of graphene oxide (GO) filled poly(lactic acid)/thermoplastic polyurethane (PLA/TPU) blends, prepared via melt mixing process, and their shape recovery and shape fixity are measured, and the results are correlated with morphology, dynamic mechanical properties, crystallinity and creep recovery behavior. Morphological analysis by scanning and transmission electron microscopy reveals that the blends are immiscible, and GO platelets are mainly localized in the TPU phase of the blends, which lead to smaller and more elongated TPU droplets with improved interfacial adhesion being responsible for the improved shape recovery performance compared to the unfilled blend. A systematic enhancement found in storage and Young's modulus, tensile strength, creep resistance and creep recovery, and cold crystallinity as a result of GO inclusion are in agreement with the improved shape recovery, shape fixity and overall shape memory performance of the filled systems. The developed PLA/TPU/GO nanocomposites with highly improved mechanical properties can be utilized as a new class of environmentally friendly shape memory materials for a broad range of applications.

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“…GO substrates confer resistance to detachment for cells under other destructive environmental cues such as shear force, indicating it is a desirable substrate for stress conditions where anoikis occurs. [ 44 ] Our transcriptome data demonstrates upregulated ECM‐related genes that may explain improved viability, including collagen, which plays a crucial role in MSC adhesion and survival under H 2 O 2 stress. [ 45,46 ] Enhancing sAD‐MSC survival in severe PNI conditions may provide a significant therapeutic advantage that merits future in vivo experiments.…”

Section: Discussionmentioning

confidence: 99%

Graphene Oxide Substrate Promotes Neurotrophic Factor Secretion and Survival of Human Schwann‐Like Adipose Mesenchymal Stromal Cells

et al. 2021

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Mesenchymal stromal cells from adipose tissue (AD‐MSCs) exhibit favorable clinical traits for autologous transplantation and can develop ‘Schwann‐like’ phenotypes (sAD‐MSCs) to improve peripheral nerve regeneration, where severe injuries yield insufficient recovery. However, sAD‐MSCs regress without biochemical stimulation and detach from conduits under unfavorable transplant conditions, negating their paracrine effects. Graphene‐derived materials support AD‐MSC attachment, regulating cell adhesion and function through physiochemistry and topography. Graphene oxide (GO) is a suitable substrate for human sAD‐MSCs incubation toward severe peripheral nerve injuries by evaluating transcriptome changes, neurotrophic factor expression over a 7‐days period, and cell viability in apoptotic conditions is reported. Transcriptome changes from GO incubation across four patients are minor compared to biological variance. Nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF), and glial‐derived neurotrophic factor (GDNF) gene expression is unchanged from sAD‐MSCs on GO substrates, but NGF and GDNF protein secretion increase at day 3 and 7. Secretome changes do not improve dorsal root ganglia neuron axon regeneration in conditioned media culture models. Fewer sAD‐MSCs detach from GO substrates compared to glass following phosphate buffer saline exposure, which simulates apoptotic conditions. Overall, GO substrates are compatible with sAD‐MSC primed for peripheral nerve regeneration strategies and protect the cell population in harsh environments.

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Flow induced HeLa cell detachment kinetics show that oxygen-containing functional groups in graphene oxide are potent cell adhesion enhancers

Abstract: Oxygen-containing functional groups in graphene oxide are potent cell adhesion enhancers.

Cited by 18 publications

References 48 publications

Fabrication and Application of Novel Porous Scaffold in Situ-Loaded Graphene Oxide and Osteogenic Peptide by Cryogenic 3D Printing for Repairing Critical-Sized Bone Defect

Fabrication and Application of Novel Porous Scaffold in Situ-Loaded Graphene Oxide and Osteogenic Peptide by Cryogenic 3D Printing for Repairing Critical-Sized Bone Defect

Influence of Graphene Oxide on Thermally Induced Shape Memory Behavior of PLA/TPU Blends: Correlation with Morphology, Creep Behavior, Crystallinity, and Dynamic Mechanical Properties

Graphene Oxide Substrate Promotes Neurotrophic Factor Secretion and Survival of Human Schwann‐Like Adipose Mesenchymal Stromal Cells

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