Flow injection and liquid chromatography detector for amino acids based on a post-column reaction with luminol

Macdonald, A.M.G.; Nieman, Timothy A.

doi:10.1021/ac00281a035

Cited by 70 publications

(18 citation statements)

References 30 publications

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“…The excited 3-aminophthalate ions return to the ground state with enhanced CL phenomena, as shown in reactions (5) and (6). However, it has been reported that amino acid could combine with Cu 2+ ions to the complexation of amino acid and Cu 2+ ions as shown in reaction (7); [33] (3) and (4)]. However, the phenomena of absorption spectra enhanced by Cu 2+ almost disappeared as Gln was added (as shown in Fig.…”

Section: Study Of Mechanismmentioning

confidence: 99%

The determination of glutamine with flow‐injection chemiluminescence detection and mechanism study

Liu

Shi

et al. 2009

Luminescence

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The main purpose of this study was to develop an inexpensive, simple, rapid and sensitive chemiluminescence (CL) method for the determination of glutamine (Gln) using a flow-injection (FI) system. Gln was found to strongly inhibit the CL signal of the luminol-H(2)O(2)-CuSO(4) system in Na(2)B(4)O(7) solution. A new FI-CL method was developed for the determination of Gln. Parameters affecting the reproducibility and CL detection were optimized systematically. Under the optimized conditions, the corresponding linear regression equation was established over the range of 5.0 x 10(-7) to 2.5 x 10(-6) mol/L with the detection limit of 1.8 x 10(-8) mol/L. The relative standard deviation was found to be 1.8% for 11 replicate determinations of 1.5 x 10(-6) mol/L Gln. The proposed method has been satisfactorily applied for the determination of Gln in real samples (Marzulene-s granules) with recoveries in the range of 98.7-108.6%. The minimum sampling rate was about 100 samples/h. The possible mechanism of this inhibitory CL was studied by fluorescence spectrophotometer and UV-vis spectrophotometer.

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Section: Study Of Mechanismmentioning

confidence: 99%

The determination of glutamine with flow‐injection chemiluminescence detection and mechanism study

Liu

Shi

et al. 2009

Luminescence

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“…Different detection schemes have been reported based on the inhibiting effects of amino acids (22,23), thiols (24,25), protein (26,27) and thioimidazole (28) on the Cu(II)-catalysed CL oxidation of luminol. Although TCs were known to form stable complexes with Cu(II) (29,30), no use of this property for analytical purposes has been reported so far.…”

Section: The Choice Of Catalyst Ion and The Detection Principlementioning

confidence: 99%

“…In addition, Cu(II) can form stable complexes with TCs (29,30) and complexed Cu(II) is generally a much poorer catalyst than the uncomplexed form of Cu(II) (22,23). Our system monitored that the Cu(II) catalyst was continuously mixed with the luminol and …”

Section: The Choice Of Catalyst Ion and The Detection Principlementioning

confidence: 99%

Determination of tetracycline, chlortetracycline and oxytetracycline by flow injection with inhibitory chemiluminescence detection using copper(II) as a probe ion

Han

Liu

2006

Luminescence

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This paper reports an indirect flow-injection (FI) method for the determination of the tetracycline drugs (TCs), tetracycline (TC), chlortetracycline (CTC) and oxytetracycline (OTC), using copper(II) as a probe ion. The method was based on the inhibition caused by these TCs to the copper(II)-catalysed chemiluminescence (CL) reaction between luminol and H(2)O(2). The CL reaction was induced on-line and injection of the sample produced negative peaks as a result of the copper(II) complexation or displacement by the analytes. The height of the peaks was proportional to the drug concentration in the sample. The choice of the catalyst ion, the concentration of luminol, H(2)O(2) and copper(II) are discussed. The linear range was 3.6 x 10(-8)-1.0 x 10(-5), 1.1 x 10(-7)-1.0 x 10(-5) and 1.9 x 10(-7)-1.0 x 10(-5) mol/L for TC, CTC and OTC, respectively. The detection limit was 5.0 x 10(-9) mol/L for TC, 1.0 x 10(-8) mol/L for CTC and 2.0 x 10(-8) mol/L for OTC (3sigma), respectively. The method was applied to the determination of TCs in pharmaceutical preparations and human urine with recoveries in the range 95-105%.

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“…The use of such aryl oxalate systems has been reviewed by Imai and Weinberger (23). A procedure utilizing the well-known luminol-peroxide chemiluminescence system has been presented by MacDonald and Nieman (24)…”

Section: Chemiluminescence Detectionmentioning

confidence: 99%

Detection Limits for Amino Acids in Environmental Samples

Hare

John²

1987

ACS Symposium Series

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Liquid chromatography techniques using fluorescent derivatives and laser-induced fluorescence can separate and detect sub-femtomole levels of amino acids. The widespread distribution of amino acids and proteins from living organisms produces nanomolar and higher levels of amino acid material in most environments of the earth's surface. Contamination usually occurs to some extent during sample collecting and processing and must be recognized and addressed before meaningful amino acid concentrations and distributions can be obtained from environmental samples. Amino acids are distributed ubiquitously throughout much of the earth's crust, including the atmosphereTheir occurrence and important role in living organisms are well known, but amino acids have also been found in fossils and rocks hundreds of millions of years and even billions of years old (2). Amino acids have been reported at parts-per-billion levels in extra-terrestrial samples such as the Apollo moon rocks as well as in several meteorites (3,4). Even distilled water, reagent-grade HC1 and other chemicals frequently contain trace amounts of amino acids. Figure 1 summarizes the levels of amino acids found in several samples of environmental interest.Current analytical techniques for the analysis of the common amino acids are able to detect less than femtomole (10~15) amounts using liquid chromatographic methods with fluorescent derivatives. However, this level of sensitivity may be extremely difficult to utilize because of the widespread presence of amino acids from living organisms. For example, the relatively high levels of amino acids in human body tissues and fluids make human fingerprints or even a person's breath a potential serious contaminant in detecting amino acids in specific environmental samples (5). Sample collecting and preparation are major concerns to amino acid trace analysis. Even with careful handling during sample collection and preparation it is always possible that the sample may have been contaminated in situ before its collection.

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Flow injection and liquid chromatography detector for amino acids based on a post-column reaction with luminol

Cited by 70 publications

References 30 publications

The determination of glutamine with flow‐injection chemiluminescence detection and mechanism study

The determination of glutamine with flow‐injection chemiluminescence detection and mechanism study

Determination of tetracycline, chlortetracycline and oxytetracycline by flow injection with inhibitory chemiluminescence detection using copper(II) as a probe ion

Detection Limits for Amino Acids in Environmental Samples

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