Flow injection spectrophotometric determination total antioxidant capacity in human serum samples based on response surface methodology to optimize synthesized peroxidase-like activity carbon dots

Zhang, Guo-Qi; Wei-Wu,; Shi, Yuhan; Zhao, Yang; Xu, Zhi-Hong

doi:10.1016/j.saa.2022.121832

Cited by 7 publications

(4 citation statements)

References 24 publications

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“…To this end, this study aims to achieve highquality peripheral blood chromosomal karyotypes by employing response surface methodology (RSM) to investigate the optimal combination of blood sample refrigeration time and blood volume. RSM, a method that combines mathematics and statistics, is used for developing, enhancing, and optimizing procedures [13,14]. As a classic multifactorial analysis method, RSM can effectively address the optimization of peripheral blood chromosomal examination methods under conditions of multifactorial interactions.…”

Section: Introductionmentioning

confidence: 99%

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Wei

2024

Am J Transl Res

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Objective: This study aims to investigate the impact of refrigeration time and blood volume on the success rate of peripheral blood chromosomal analysis using response surface methodology (RSM). Methods: Peripheral blood samples from 30 volunteers were subjected to chromosomal analysis under different refrigeration duration periods (≤7 days, 8 days, 9 days, 10 days, 11 days, 12 days, 13 days, 14 days) along with different blood volumes (0.2 mL, 0.3 mL, 0.4 mL, 0.5 mL, 0.6 mL, 0.7 mL, and 0.8 mL). The effects of refrigeration time and blood volume on the success rate of peripheral blood chromosomal analysis were determined using the Chi-square test for trend, followed with Spearman's rank correlation coefficient, and RSM analysis to identify the optimal combination of refrigeration time and blood volume. Results: The refrigeration time within 10 days had a minor impact on the success rate, while refrigeration time more than 11 days significantly decreased the success rate. An increase in blood volume slightly improved the success rate. The success rate showed both linear and nonlinear changes with refrigeration time, while the effect of blood volume was primarily linear. The highest success rate was observed at a refrigeration time of ≤7 days and a blood volume of 0.8 mL. The interaction between refrigeration time and blood volume had a significant impact on the success rate. Conclusion: It is recommended to keep the refrigeration time of blood samples within 7 days and control the blood volume at 0.8 mL to maximize the success rate of chromosomal analysis.

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Section: Introductionmentioning

confidence: 99%

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Wei

2024

Am J Transl Res

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“…In general, antioxidants, notably cysteine (Cys), glutathione (GSH), and ascorbic acid (AA) maintain the redox homeostasis of cells and tissues . However, because of the wide range of antioxidants and their probable interactions, the analysis of individual antioxidants is challenging.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Peroxidase-Mimic Catalytic Activity via Cerium Doping of Strontium-Based Metal–Organic Frameworks with Design of a Smartphone-Based Sensor for On-Site Salivary Total Antioxidant Capacity Detection in Lung Cancer Patients

Alshatteri

Ali

Omer

2023

ACS Appl. Mater. Interfaces

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The development of artificial nanozymes with superior catalytic performance and excellent stability has been a long-standing objective for chemists. The total antioxidant capacity (TAC) is one of the most important bioanalytical measures of oxidative stress in the body. The present work aims to develop a smartphone-assisted visual detection sensor using cerium-doped strontium-based metal–organic frameworks (Ce-SrMOFs) as peroxidase-like nanozymes for the rapid, low-cost, on-site detection of TAC. The pristine SrMOF functioned as a peroxidase nanozyme, and its enzymatic activity was enhanced after doping it with Ce(IV) ions because of the multivalent nature and synergistic impact of the heteroatoms. The Ce-SrMOFs were sensitive to the single electron transfer and hydrogen atom transfer processes, which implies that the Ce-SrMOFs can serve as an ideal nanozyme candidate for TAC analysis. The investigated mechanism revealed that •OH is the most active oxygen species for the peroxidase-like activity. The Ce-SrMOFs exhibited a strong affinity for 3,3′,5,5′-tetramethylbenzidine (TMB) and H2O2, with K m values of 0.082 and 0.427 mM, which are 5.29- and 8.67-fold lower than those of horseradish peroxidase (HRP), respectively. The Ce-SrMOFs were used for the detection of ascorbic acid, cysteine, and glutathione, with limits of detection of 44, 53, and 512 nM, respectively. The proposed method proved effective in measuring the TAC in saliva samples from lung cancer patients, thereby yielding results with satisfactory precision and accuracy.

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“…In general, the cellular redox homeostasis highly relies on the intake of antioxidants, notably cystine (Cys), glutathione (GSH), and AA . However, effective detection of individual antioxidants is challenging, due to the potential interaction .…”

Section: Introductionmentioning

confidence: 99%

“…7 In general, the cellular redox homeostasis highly relies on the intake of antioxidants, notably cystine (Cys), glutathione (GSH), and AA. 8 However, effective detection of individual antioxidants is challenging, due to the potential interaction. 7 Herein, the total antioxidant capacity (TAC) is proposed to indicate the antioxidant capacity 9 and implies the content of ROS that a test solution can scavenge from samples of various natural sources.…”

Section: Introductionmentioning

confidence: 99%

Red-Emissive Carbon Dots Combined with Ferric Ions for Detection of Total Antioxidant Capacity in Food

Luo

Liu

Sun

et al. 2023

ACS Appl. Nano Mater.

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In general, the total antioxidant capacity (TAC) in food is vital for evaluating the quality of antioxidant food and health monitoring. Most studies about the access of the TAC in food are mainly focused on typical colorimetric strategies. Herein, a simple and sensitive strategy for TAC in beverages and fruit has been proposed based on amino/hydroxyl-functionalized carbon dots (N 2 H 4 -CDs) and Fe 3+ . The fluorescence (FL) of N 2 H 4 -CDs at 650 nm can be effectively and selectively quenched via the formation of the nonfluorescence complex (Fe 3+ /N 2 H 4 -CDs). Moreover, the FL of N 2 H 4 -CDs is concentrated in the red region, which can eliminate the interference in complex biological environments. Interestingly, based on the theoretical calculations of density functional theory (DFT), we have confirmed that the surface amino and hydroxyl play an essential role in selective association with Fe 3+ , resulting in effective quenching. Moreover, because Fe 3+ can be reduced by antioxidants to Fe 2+ , the quenched FL of N 2 H 4 -CDs can be restored in the presence of ascorbic acid (AA) and glutathione (GSH). Therefore, the typical antioxidants (AA) and especially certain thiol antioxidants (GSH) can also be analyzed by the proposed strategy. More importantly, we have proved that this strategy is successfully utilized for analyzing the TAC in beverages and fruit.

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Flow injection spectrophotometric determination total antioxidant capacity in human serum samples based on response surface methodology to optimize synthesized peroxidase-like activity carbon dots

Cited by 7 publications

References 24 publications

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Optimizing peripheral blood chromosome analysis: effects of refrigeration time and blood volume

Enhanced Peroxidase-Mimic Catalytic Activity via Cerium Doping of Strontium-Based Metal–Organic Frameworks with Design of a Smartphone-Based Sensor for On-Site Salivary Total Antioxidant Capacity Detection in Lung Cancer Patients

Red-Emissive Carbon Dots Combined with Ferric Ions for Detection of Total Antioxidant Capacity in Food

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