Flow injection system for potentiometric determination of alkaline phosphatase inhibitors

Koncki, Robert; Rudnicka, Katarzyna; Tymecki, Łukasz

doi:10.1016/j.aca.2006.05.100

Cited by 29 publications

(11 citation statements)

References 14 publications

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“…Taking into account that the enzymatic activity of ALPs is inhibited by vanadium [18,20,25], the presence of this metal into the electrochemical cell results in a current decrease. In this way, the difference between the steady-state current in the absence of vanadium (I 0 ) and the steady-state current in the presence of vanadium (I) (ΔI (I 0 –I)) can be quantitatively related to concentration of vanadium added.…”

Section: Resultsmentioning

confidence: 99%

“…ALP is probably the most commonly used conjugated enzyme for immunoassays due to its high turnover number, broad substrate specificity and possibility of application. The determination of its activity has been carried out using various spectrophotometric and electrochemical methods [ 21 – 25 ]. In the development of sensitive electrochemical ALP-based assays stable substrates such as phenyl phosphate [ 26 – 28 ], naphthyl phosphate [ 28 , 29 ], ascorbic acid 2-phosphate [ 28 , 30 ], p -nitrophenyl phosphate [ 28 , 31 ] and rivoflavin-5-monophosphate [ 20 ] have been used.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination

Alvarado-Gámez

Alonso‐Lomillo

Domínguez‐Renedo

et al. 2014

Sensors

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A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 μM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination

Alvarado-Gámez

Alonso‐Lomillo

Domínguez‐Renedo

et al. 2014

Sensors

View full text Add to dashboard Cite

show abstract

“…Among the other enzymes reported in Table 1 with an ion selective electrode as sensor by Koncki et al, 2006. In the case of free peroxidase enzyme, two substrates for its reaction in solution (hydrogen peroxide and phenol derivative such as hydroquinone) are required. The possible mechanism of peroxidase sensor is as follows: Where HRP is peroxidase, BQ is benzoquinone and QH 2 is hydroquinone, compound I and II denote enzyme intermediates in the reaction.…”

Section: Enzymes Employed In Biosensors Based On Enzyme Inhibitionmentioning

confidence: 99%

Recent advances in biosensors based on enzyme inhibition

Amine

Arduini

Moscone

et al. 2016

Biosensors and Bioelectronics

190

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“…Koncki et al (2006) developed a simple flow injection system for potentiometric detection of ALP activity. The researchers used monofluorophosphate as an ALP specific substrate [112]. The use of the substrate helped in improving the application of fluoride ion selective electrode in detecting enzyme-catalyzed reactions.…”

Section: Electrochemistry Detection Techniquesmentioning

confidence: 99%

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Balbaied

Moore

2019

Biosensors

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Alkaline phosphatase (ALP), which catalyzes the dephosphorylation process of proteins, nucleic acids, and small molecules, can be found in a variety of tissues (intestine, liver, bone, kidney, and placenta) of almost all living organisms. This enzyme has been extensively used as a biomarker in enzyme immunoassays and molecular biology. ALP is also one of the most commonly assayed enzymes in routine clinical practice. Due to its close relation to a variety of pathological processes, ALP’s abnormal level is an important diagnostic biomarker of many human diseases, such as liver dysfunction, bone diseases, kidney acute injury, and cancer. Therefore, the development of convenient and reliable assay methods for monitoring ALP activity/level is extremely important and valuable, not only for clinical diagnoses but also in the area of biomedical research. This paper comprehensively reviews the strategies of optical and electrochemical detection of ALP and discusses the electrochemical techniques that have been addressed to make them suitable for ALP analysis in cell culture.

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Flow injection system for potentiometric determination of alkaline phosphatase inhibitors

Cited by 29 publications

References 14 publications

A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination

A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination

Recent advances in biosensors based on enzyme inhibition

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

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