Flow sandwich immunoassay for specific anti-OVA IgG antibody by use of surface plasmon resonance sensor

Li, Yan; Ren, Jujie; Nakajima, Hirochika; Kim, Byung Kyu; Soh, Nobuaki; Nakano, Koji; Iwamoto, Toshihiko

doi:10.1016/j.talanta.2008.03.007

Cited by 10 publications

(5 citation statements)

References 19 publications

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“…however, unraveling the kinetic aspects of this assay [34,[53][54][55][56] can be challenging because the Ab competitively binds either to immobilized antigen or antigen in the sample solution.…”

Section: Detection Of Clenbuterolmentioning

confidence: 99%

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Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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Surface plasmon resonance sensor equipped with a fabricated immunosensor chip is used for detecting clenbuterol in this study. Since clenbuterol is a small analyte, indirect competitive inhibition immunoassay is employed. For fabricating the immunosurface, the Au-chip was functionalized by succinimidyl-terminated alkanethiol, and the terminal N-hydroxysuccinimide group of the self-assembled monolayer was either replaced with clenbuterol or blocked with ethanolamine. Scanning tunneling microscope experiments and electrochemical measurements depicted the domain structures of the succinimide group of succinimidyl-terminated propanethiol monolayer. The surface concentration and the orientation of succinimide group was significantly dependent on the concentration of dithiobis(succinimidyl) propionate (DSP) used in fabricating the monolayer. Furthermore, the structure of monolayer significantly influenced both the surface concentration and the orientation of clenbuterol on the sensor surface. Consequently, high coverage and standing-up configuration of clenbuterol showed high affinity for clenbuterol antibody. However, high affinity constant exhibited by the sensor surface was coupled with a low sensitivity. By contrast, lowest concentration of DSP solution (0.1 mM) used in fabricating the immunosurface showed a detection sensitivity of 3 ppt-the highest reported sensitivity for clenbuterol. For regeneration the immunosurface, 0.1 M NaOH was used and the same sensor surface could be reused for performing >100 rapid immunoreaction.

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Section: Detection Of Clenbuterolmentioning

confidence: 99%

“…Moreover, <5% error is observed between the results obtained using different sensor chips (N > 3). According to reported protocols [34,[53][54][55][56], the affinity constant (K 1 ) of Ab to the clenbuterol (antigen) immobilized on the sensor surface can be determined using a Langmuirtype adsorption model (Fig. 6(a)).…”

Section: Detection Of Clenbuterolmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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“…Remarkably, an LOD as low as 2 nM has been achieved. Therefore, the SPR assay developed herein, encompassing a bare physisorbed layer of anti-S1 capture antibodies, is among the best-performing SPR assays reported so far in terms of LOD and selectivity, 19,20,66 being also cost-effective, more rapid than other immobilization techniques, and avoiding the use of coupling reagents. Indeed, an assay encompassing an SPR slide modified with a short hetero-functional poly(ethylene glycol) (PEG) linker, HS-PEG6-COOH, followed by covalent coupling with VHH-72-Fc nanobodies using a standard amide coupling procedure (NHS/EDC) to detect SARS-CoV-2 S1 proteins, recently achieved an LOD of 10 nM and a selectivity ratio Dy NC /Dy Sens of about 0.13, one order of magnitude higher than that achieved in the present study.…”

Section: Papermentioning

confidence: 99%

A stable physisorbed layer of packed capture antibodies for high-performance sensing applications

et al. 2023

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“…Each immunoreaction-regeneration cycle took only 1000 s. were determined using a Langmuir-type adsorption mode [42][43][44][45][46]. K 2 , which is defined as the secondary affinity constant K 1 of Ab to the free clenbuterol in the solution, was also determined.…”

Section: Detection Of Clenbuterolmentioning

confidence: 99%

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

Suherman

Morita

Kawaguchi

2015

Sensors and Actuators B: Chemical

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This work aims to find the key factor determining the detection limit for an indirect competitive inhibition immunoassay using surface plasmon resonance (SPR) sensing. In our previous work, the thiol solution concentration used in a self-assembly process highly affected the alkanethiol monolayer structure on the sensor surface [Suherman et al., 2014]. It was noticed that the monolayer structure determined the immunoassay sensitivity due to the orientation and the surface concentration of antigen in domain structure of monolayer. To study the effect of orientation of antigen, we examined here three types of alkanethiol compounds: dithiobis(succinimidyl undecanoate) (DSU; straight-chain alkanethiol), carboxy-EG 6 -undecanethiol (CEG 6 ; flexible-chain alkanethiol), and(C 2 -NTA; three-branched alkanethiol). By electrochemical reductive desorption, the surface concentration of DSU, CEG 6 , and C 2 -NTA were estimated to be 6.6 × 10 -10 , 8.5 × 10 -10 , 4.6 × 10 -10 mol cm -2 , respectively. Subsequently, SPR suggested that the ratios of immobilized antigen (clenbuterol) per thiol were 0.13, 0.16, and 0.15 for DSU, CEG 6 , and C 2 -NTA, respectively. This suggests that the amount of immobilized clenbuterol was defined by the molecular size of clenbuterol. Sensor surface structures on a molecular scale were evaluated using high-resolution scanning tunneling microscopy (STM), which showed tilted clenbuterol with θ DSU > θ CEG6 > θ C2-NTA . For SPR sensing of clenbuterol, C 2 -NTA showed the highest sensitivity (LOD = 10 ppt) among the examined alkanethiols, because clenbuterol formed a laid-on structure. Based on kinetics, the key factor for sensing performance is discussed.2

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Flow sandwich immunoassay for specific anti-OVA IgG antibody by use of surface plasmon resonance sensor

Cited by 10 publications

References 19 publications

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

A stable physisorbed layer of packed capture antibodies for high-performance sensing applications

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

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