Flow-Through Multianalyte Chemiluminescent Immunosensing System with Designed Substrate Zone-Resolved Technique for Sequential Detection of Tumor Markers

Fu, Zhifeng; Liu, Hong; Ju, Huangxian

doi:10.1021/ac0610560

Cited by 103 publications

(59 citation statements)

References 41 publications

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“…Labeled probe methods such as multilabel and spatially resolved assays can offer amplified detection signals for multianalyte immunoassays. Multilabel methods need several labels, such as radioisotopes (15 ), fluorescence dyes (16 ), enzymes (17,18 ), metal ions (12,14,19 ), or quantum dots (20,21 ), which limits their application. Spatially resolved array assays with a single label can perform simultaneous detection of analytes by both optical (22)(23)(24) and electrochemical (25 ) responses.…”

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A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

et al. 2007

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Background: Automated and convenient multianalyte detection with high throughput is increasingly needed in clinical diagnosis. We developed a disposable 4-by-2 array for programmed simultaneous amperometric immunoassay of 4 tumor markers. Methods: We used a screen-printed technique, 1-step immobilization method, and flow injection technique. We immobilized carcinoembryonic antigen, ␣-fetoprotein, ␤-human choriogonadotropin, and carcinoma antigen 125 as model analytes in a redox mediator-grafted, biopolymer-modified, screen-printed carbon electrode array to capture corresponding horseradish peroxidaselabeled antibodies in competitive immunoreactions. The simultaneous multianalyte immunoassay was automatically carried out to amperometrically monitor the mediator-catalyzed enzymatic response to hydrogen peroxide, which decreased in proportion to the concentrations of analytes in samples. Results: The multianalyte immunosensor array had a throughput of 60 samples/h and allowed simultaneous detection of carcinoembryonic antigen, ␣-fetoprotein, ␤-human choriogonadotropin, and carcinoma antigen 125 in clinical serum samples with concentrations up to 188 g/L, 250 g/L, 266 IU/L, and 334 kIU/L, respectively. The detection limits (limits of the blank, mean of blank plus 3 SD) were 1.1 g/L, 1.7 g/L, 1.2 IU/L, and 1.7 kIU/L. The inter-and intraassay imprecision (CVs) of the immunosensor arrays were <7.8% and <9.0%, respectively. The immunosensor arrays were stable for 28 days.

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A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

et al. 2007

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“…Based on this fact, they developed an expression hybridization assay that combines firefly-luciferase DNA with Renilla-luciferase DNA as labels for the simultaneous determination of two target DNA sequences. Fu et al 105 designed a strategy for a substrate zone-resolved multianalyteimmunoassay for the detection of CA125 and CEA by employing HRP and AP as labels, respectively. Under the optimal conditions, CA 125 and CEA could be assayed in the ranges of 5.0 -100 units/mL and 1.0 -120 ng/mL, respectively.…”

Section: ·2 Substrate Zone-resolved Techniquementioning

confidence: 99%

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Fan

Cao

et al. 2009

ANAL. SCI.

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IntroductionCL is the production of electromagnetic radiation by a chemical reaction. The CL intensity is directly proportional to the concentration of a limiting reactant involved in the CL reaction. CL has been exploited for its use in a wide range of applications due to its high sensitivity, wide calibration range, and suitability for miniaturization. Protein and DNA analyses have recently attracted much attention. Many different types of techniques, such as electrochemical, optical, radiochemical, and piezoelectronic methods have been applied for protein and DNA analyses. To provide an overview of CL detection assays for DNAs and proteins, we have summarized their essential techniques. Monoplex and multiplex assays are described, and their current reports are intended to provide an overview of the CL techniques currently employed in numerous laboratories. Monoplex AssayA monoplex assay aims to measure the presence of a single analyte in any given sample, in which nanoparticle, DNA enzyme, horseradish peroxidase (HRP), alkaline phosphatase (AP) and acridinium ester are commonly used as labels. 2·1 Nanoparticle-based CL techniquesNanoparticles offer excellent prospects for DNA detection and immunoassay, owing to their many attractive properties. Compared to existing labels, nanoparticles are more stable and cheaper, and easier to be purified. In addition, they indicate faster binding kinetics with high sensitivity and a high reaction rate for many types of monoplex assays, ranging from immunoassays to DNA detection. 2·1·1 Gold nanoparticlesColloidal gold labels are ideal in biotechnological systems for several reasons. Firstly, they can be readily prepared in a wide range of sizes, from approximately 2 nm to above 100 nm. Secondly, the biochemical activity of the labeled biomolecules can be retained when colloidal gold is coupled to the biomolecules and, thirdly, colloidal gold labels can be easily visualized as dense structures within biological entities using transmission electron microscopy. As a biological tag, colloidal gold has been employed in several CL detection systems. 2·1·1·1 Gold nanoparticle-based CL immunoassay. The Au 3+ -luminol reaction, in which 10 -9 M Au 3+ ions can be sensitively detected, is a classic CL reaction. Thousands of Au atoms are contained within the gold nanoparticles; for example, 2.3 ¥ 10 5 Au atoms are theoretically contained in a 20-nm spherical gold particle and, consequently, picomolar detection limits can be attained by employing oxidative gold metal dissolution in acidic solutions. Our group 1,2 reported a sensitive CL immunoassay based on a colloidal gold label after oxidative gold metal dissolution (using 0.01 M HCl-0.5 M NaCl-0.5 mM Br2), using a simple and sensitive luminol-CL reaction (Fig. 1). Reviews Chemiluminescence Platforms in Immunoassay and DNA AnalysesAiping FAN,* Zhijuan CAO,* Huan LI,* Masaaki KAI,** and Jianzhong LU* † *School of Pharmacy, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China ** Faculty of Pharmaceutical Sciences, Graduate Scho...

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“…Thus, multilabel assays and spatially resolved assay systems have been developed as the main modes to perform SMAT (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25). Application of the multilabel assays has been limited by difficulty in accurate quantification due to different optimal assay conditions and the signal overlap of different labels (11)(12)(13).…”

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“…Application of the multilabel assays has been limited by difficulty in accurate quantification due to different optimal assay conditions and the signal overlap of different labels (11)(12)(13). Although a set of substrate zone-resolved techniques have been proposed to overcome these drawbacks (14,15 ), the restriction in the number of available labels still greatly limits their application.…”

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Disposable Reagentless Electrochemical Immunosensor Array Based on a Biopolymer/Sol-Gel Membrane for Simultaneous Measurement of Several Tumor Markers

Yan

Zhang

et al. 2008

Clinical Chemistry

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Flow-Through Multianalyte Chemiluminescent Immunosensing System with Designed Substrate Zone-Resolved Technique for Sequential Detection of Tumor Markers

Cited by 103 publications

References 41 publications

A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

A Disposable Multianalyte Electrochemical Immunosensor Array for Automated Simultaneous Determination of Tumor Markers

Chemiluminescence Platforms in Immunoassay and DNA Analyses

Disposable Reagentless Electrochemical Immunosensor Array Based on a Biopolymer/Sol-Gel Membrane for Simultaneous Measurement of Several Tumor Markers

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