Flowcytometric comparative analysis in acute leukemias between Indian and proposed minimal screening panel

Gupta, Srishti; Chatterjee, Tathagata; Sharma, Sanjeevan; Sharma, Ajay; Ganguly, Prosenjit; Singh, Jasjit; Das, Satyaranjan

doi:10.1016/j.mjafi.2016.01.002

Cited by 3 publications

(3 citation statements)

References 40 publications

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“…22,23 Even with the limited panel markers diagnosing ambiguous cases of MPAL can be made possible. 24 In our study, we employed a selective approach in choosing an antibody panel based on the initial morphological and cytochemical assessment. In cases of Acute leukemia, the strategy of using CD45 as the only anchor marker enabled us to access four markers per tube and also led to a decrease in the usage of reagents.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic utility of flow cytometry in resource-constrained settings- a single center experience

Mir

Mushtaq

Hameed

et al. 2023

Preprint

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Background:Flow cytometry (FC) is an expensive investigation that is unaffordable for many patients from underprivileged nations. We aimed to reduce the total budget of the test by optimizing a limited antibody panel as guided by morphological assessment. Materials and methods:This single-center, experimentalstudy included 247 patients referred for Flow cytometry at Rehman Medical Institute, Peshawar.There were 162 male (65.4 %) and 85 female patients (34.6%) with a mean age of 38.78±21.55 years. Bone marrow or peripheral blood samples were used for immunophenotyping. After the morphological assessment, a limited panel of flow markers was selected. All samples were stained with fluorochromes labeled monoclonal antibodies, acquired, and analyzed with a flow cytometer (Cytoflex, Beckman-Coulter, USA). Data were statistically analyzed through SPSS-22, and descriptive statistics were applied for all variables. All results were presented in form of graphs, tables, and charts Results:Out of 247 cases, an Acute leukemia panel was requested for 128 cases, a lymphoproliferative panel for 56 cases, T-cell subset analysis for 53 cases, and PNH clone screening for 10 cases. Newly diagnosed cases were 221 while 26 cases were known labeled cases of Acute leukemia for Minimal Residual Disease (MRD) analysis. Among the cases for MRD analysis, 10 cases were in remission, 12 cases relapsed and 04 cases were not in the remission phase. 98% of cases referred for Acute leukemia and Lymphoproliferative disorders, and 100% of cases referred for proximal nocturnal Hemoglobinuria (PNH) and T-cell subset analysis was conclusively diagnosed by our limited flow cytometry panels with low-cost effect. Conclusions:In resource-constrained settings, optimization of a limited antibody panel is extremely facilitating to provide a Flow cytometry-based diagnosis which further guides the treatment decision.

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Section: Discussionmentioning

confidence: 99%

Diagnostic utility of flow cytometry in resource-constrained settings- a single center experience

Mir

Mushtaq

Hameed

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…In this setting, screening with the use of a limited number of antibody can help in reducing the financial burden of flow cytometry. 9,[11][12][13]…”

Section: Discussionmentioning

confidence: 99%

“…A limited panel of nine ( 9) antibodies (CD45, CD19, CD10, CD20, HLA-DR, CD34, cCD3, cCD79a and cTdT) were selected. 4,12,14 Using this panel, we retrospectively reviewed all newly-diagnosed pediatric (0-18 years old) cases of B-cell and T-cell acute lymphoblastic leukemia from September 2016 to December 2019.…”

Section: Methodsmentioning

confidence: 99%

Cost-Effectiveness of Limited Screening Panel for Acute Lymphoblastic Leukemia Diagnosis in a Resource-Limited Setting

Medalla¹,

Gepte²,

Pido³

et al. 2021

Phil Journal Path

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Background. Flow cytometry is an invaluable tool in the diagnostic evaluation of acute leukemia and post therapy monitoring; however, majority of Filipino population cannot afford the cost. The use of a minimal screening panel which is both cost-effective and provides an accurate diagnosis of acute lymphoblastic leukemia is seen as an alternative.Objectives. We aim to determine the cost-effectiveness and accuracy of using a minimal screening panel for the diagnosis of acute lymphoblastic leukemia (ALL).Methodology. We selected a limited panel of 9 antibodies comprising of CD45/CD19/CD20/CD10/HLA-DR/ CD34/cCD3/cCD79a/cTdt and retrospectively reviewed newly diagnosed cases of B-cell and T-cell ALL from September 2016 to December 2019 using this panel. Results. Out of 719 bone marrow aspirates submitted for basic leukemia flow cytometric analysis we identified 268 ALL cases (239 B-ALL and 29 T-ALL).In all cases, a diagnosis was established using the limited panel. Compared to the current cost of our comprehensive panel (₱ 9,903.60). This limited panel cost ₱ 3,062.29, that offers a 69.08% savings per test, which translated to a ₱1.2 million savings a year (for an average of 180 annual cases). Conclusion.We underscore the utility of a limited panel for the diagnosis of ALL. Although this panel remains to be assessed with a larger validation cohort, its application in resource-limited developing countries is diagnostically useful and cost-effective.Recommendation. The use of a limited panel of 9 antibodies is recommended as a screening panel for patients who are highly suspected of having ALL both clinically and initial bone marrow smear assessment.

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Diagnostic utility of flow cytometry in resource-constrained settings- a single center experience

Khattak

Mushtaq

Yousafzai

et al. 2022

Preprint

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Background: Flow cytometry (FC) is an expensive investigation that is unaffordable for many patients from underprivileged nations. We aimed to reduce the total budget of the test by optimizing a limited antibody panel as guided by morphological assessment. Materials and methods: This single-center, the experimental study included 247 patients referred for Flow cytometry at Rehman Medical Institute, Peshawar.There were 162 male (65.4 %) and 85 female patients (34.6%) with a mean age of 38.78±21.55 years. Bone marrow or peripheral blood samples were used for immunophenotyping. After the morphological assessment, a limited panel of flow markers was selected. All samples were stained with fluorochromes labeled monoclonal antibodies, acquired, and analyzed with a flow cytometer (Cytoflex, BeckMan-Coulter, USA). Data were statistically analyzed through SPSS-22, and descriptive statistics were applied for all variables. All results were presented in form of graphs, tables, and charts Results: Out of 247 cases, an Acute leukemia panel was requested for 128 cases, a lymphoproliferative panel for 56 cases, T-cell subset analysis for 53 cases, and PNH clone screening for 10 cases. Newly diagnosed cases were 221 while 26 cases were known labeled cases of Acute leukemia for Minimal Residual Disease (MRD) analysis. Among the cases for MRD analysis, 10 cases were in remission, 12 cases relapsed and 04 cases were not in the remission phase. 98% of cases referred for Acute leukemia and Lymphoproliferative disorders, and 100% of cases referred for proximal nocturnal Hemoglobinuria (PNH) and T-cell subset analysis was conclusively diagnosed by our limited flow cytometry panels with low-cost effect. Conclusions: In resource-constrained settings, optimization of a limited antibody panel is extremely facilitating to provide a Flow cytometry-based diagnosis which further guides the treatment decision.

show abstract

Flowcytometric comparative analysis in acute leukemias between Indian and proposed minimal screening panel

Cited by 3 publications

References 40 publications

Diagnostic utility of flow cytometry in resource-constrained settings- a single center experience

Diagnostic utility of flow cytometry in resource-constrained settings- a single center experience

Cost-Effectiveness of Limited Screening Panel for Acute Lymphoblastic Leukemia Diagnosis in a Resource-Limited Setting

Diagnostic utility of flow cytometry in resource-constrained settings- a single center experience

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