Flower-like ZnO nanostructure based electrochemical DNA biosensor for bacterial meningitis detection

Tak, Manvi; Gupta, Vinay; Tomar, Monika

doi:10.1016/j.bios.2014.03.036

Cited by 139 publications

(57 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the current response decreases after hybridization of the TDNA because of lesser interaction of MB with unpaired guanine. The similar analyses have been reported earlier as well 34,35 . Figure 6(c) shows a linear variation in peak currents with the concentrations of target nucleotide.…”

Section: Resultssupporting

confidence: 91%

Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Singhal

Khanuja

Chaudhary

et al. 2018

Sci Rep

100

View full text Add to dashboard Cite

Development of platforms for a reliable, rapid, sensitive and selective detection of chikungunya virus (CHIGV) is the need of the hour in developing countries. To the best of our knowledge, there are no reports available for the electrochemical detection of CHIGVDNA. Therefore, we aim at developing a biosensor based on molybdenum disulphide nanosheets (MoS2 NSs) for the point-of-care diagnosis of CHIGV. Briefly, MoS2 NSs were synthesized by chemical route and characterized using scanning electron microscopy, transmission electron microscopy, UV-Vis spectroscopy, Raman spectroscopy and X-Ray Diffraction. MoS2 NSs were then subjected to physical adsorption onto the screen printed gold electrodes (SPGEs) and then employed for the detection of CHIGV DNA using electrochemical voltammetric techniques. Herein, the role of MoS2 NSs is to provide biocompatibility to the biological recognition element on the surface of the screen printed electrodes. The detection strategy employed herein is the ability of methylene blue to interact differentially with the guanine bases of the single and double-stranded DNA which leads to change in the magnitude of the voltammetric signal. The proposed genosensor exhibited a wide linear range of 0.1 nM to 100 µM towards the chikungunya virus DNA.

show abstract

Section: Resultssupporting

confidence: 91%

Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Singhal

Khanuja

Chaudhary

et al. 2018

Sci Rep

100

View full text Add to dashboard Cite

show abstract

“…A number of works published on the direct immobilization of biomolecules on the ZnO surface refers to the electrostatic interaction between ZnO and the adsorbed species [86,90,32,52]. Klaumunzer et al reported on BSA adsorption on the ZnO nanostructures.…”

Section: Direct Immobilization Of Biomolecules On Top Of Zno Nanostrumentioning

confidence: 99%

“…Mostly nanorods (NRs) [36,38,40,43,45,47,48,53,56,74,82,86] and nanowires (NWs) [29,33,42,46,49,75,77,94] were used as a biosensor platform. Nanoparticles (NPs) [35,37,50,83,84,97,98], quantum dots [34,44], other low-dimentional structures [30,32,39,52,70,71,76,79,80,81,85,89,93,95,99] and thin films [31,51,72,73] were also applied. [57], b) SEM images of ZnO nanowires grown on sapphire (0001) substrate 2 …”

Section: Application Of Zno Nanostructures In Biosensorsmentioning

confidence: 99%

Optical biosensors based on ZnO nanostructures: advantages and perspectives. A review

Tereshchenko

Bechelany

Viter

et al. 2016

Sensors and Actuators B: Chemical

282

206

View full text Add to dashboard Cite

“…Recently, ZnO nanostructures (NSs) in flower-like shape were investigated to the detection of the bacterial meningitis by electrochemical measurement system, [13]. A biosensor based on ZnO-carbon nanotubes nanocomposite was used to the meningitis DNA detection via EIS measurements, [14].…”

Section: Introductionmentioning

confidence: 99%

The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA detection based on tellurium doped ZnO nanowires

et al. 2019

View full text Add to dashboard Cite

The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA (HPV DNA) detection based on tellurium doped ZnO nanowires was fabricated. The NWs were grown by hybrid thin film oxidation in the physical vapor deposition (PVD) mechanism. The morphology characterization of the synthesized NWs was performed by field emission scanning electron microscopy (FESEM) and the images demonstrated that the diameter and the length of the materialized NWs were around 50 nm and several micrometers, respectively. The high-resolution transmission electron microscopy (HRTEM) image indicated that the fabricated NWs were crystalline and their phase characterization was validated by the X-ray diffraction pattern (XRD pattern). The single-stranded DNA (ss DNA) probe was immobilized on the surface of the Te-ZnO NWs. The electrochemical impedance spectra (EIS) measurements showed high response sensitivity after hybridization with complementary oligonucleotides. The biosensor could distinguish complementary target from non-complementary and mismatch oligonucleotides. The HBV biosensor could respond to complementary target in the concentrations range from 1 pM to 1 μM. The limit of detection (LOD) of the biosensor was 0.1 pM. The stability of the HBV DNA biosensor was investigated and biosensor could show 95% of its initial responses after 8 weeks maintenance.

show abstract

Flower-like ZnO nanostructure based electrochemical DNA biosensor for bacterial meningitis detection

Cited by 139 publications

References 41 publications

Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Detection of chikungunya virus DNA using two-dimensional MoS2 nanosheets based disposable biosensor

Optical biosensors based on ZnO nanostructures: advantages and perspectives. A review

The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA detection based on tellurium doped ZnO nanowires

Contact Info

Product

Resources

About