Flower, seed, and fruit development in three Tunisian species of Polygonum: Implications for their taxonomy and evolution of distyly in Polygonaceae

Mahmoudi, Maher; Boughalleb, Fayçal; Pellegrino, Giuseppe; Abdellaoui, Raoudha; Nasri, Nizar

doi:10.1371/journal.pone.0227099

Cited by 12 publications

(7 citation statements)

References 49 publications

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“…In addition, the largest genus of Polygonaceae was Polygonum L . The Polygonum species were woody‐stocked annual and perennial herbs, subshrubs, or shrubs [65] . Before germination begins, P. equisetiforme seeds needs to be stored for a long time (over two months).…”

Section: Resultsmentioning

confidence: 99%

“…[63,64] In addition, the largest genus of Polygonaceae was Polygonum L. The Polygonum species were woody-stocked annual and perennial herbs, subshrubs, or shrubs. [65] Before germination begins, P. equisetiforme seeds needs to be stored for a long time (over two months). The ideal temperature for seed germination is 15 °C, as stated by Bidak et al [66] This plant kind can survive for a very long time.…”

Section: Key Species Domestication and Cultivationmentioning

confidence: 99%

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Phenolic Profiling, Antioxidant, and Antibacterial Activities of Selected Medicinal Plants from Tunisia

Hamrouni

Romdhane

Benmoussa

et al. 2023

Chemistry & Biodiversity

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Phytochemical screening of aqueous extract from six medicinal wild plants grown in South‐eastern of Tunisia: Atriplex halimus, Teucrium polium, Moricandia arvensis, Deverra tortuoa, Haplophyllum tuberculatum and Polygonum equisetiforme were evaluated. Both decoction and ultrasound assisted extraction were used. Antioxidant, antibacterial proprieties, and phenolic profiling, using LC‐ESI‐MS method, were assessed. Total polyphenols, flavonoids, and condensed tannins contents ranged from 7.47±0.19 to 22.25±0.49 mg GAE/g Dw, 5.47±0.06 to 7.55±0.07 mg RE/g Dw, and 0.33±0.02 to 19.43±0.64 mg TAE/g Dw, respectively. Moreover, the reducing power and DPPH tests showed that P. equisetiforme (EC50: 12.50±0.50 μg/ml; DPPH⋅+: 213.49±4.24 mg TEAC/g DW), T. polium (EC50: 25.00±1.00 μg/ml; DPPH⋅+: 181.39±9.47 mg TEAC/g DW) as well as H. tuberculatum (EC50: 56.25±0.25 μg/ml; DPPH⋅+: 177.83±5.85 mg TEAC/g DW) extracts were the most effective natural antioxidants. For anti‐bacterial activity, the ultrasonic extract of H. tuberculatum showed the highest activity against both P. aeruginosa (13.50±0.71 mm) and S. aureus (13.00±0.00 mm) at 10 mg/ml. Furthermore 24 phenolic compounds were identified, with predominance of quinic acid, gallic acid, protocatechuic acid, syringic acid, p‐coumaric acid, trans‐ferulic acid, catechin (+), trans‐cinnamic and silymarin. These results were further consolidated by to heatmap clustering with P. equisetiforme, H. tuberculatum, T. polium as the main antioxidant and antibacterial sources which supports their domestication and industrial use.

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Section: Resultsmentioning

confidence: 99%

Section: Key Species Domestication and Cultivationmentioning

confidence: 99%

Phenolic Profiling, Antioxidant, and Antibacterial Activities of Selected Medicinal Plants from Tunisia

Hamrouni

Romdhane

Benmoussa

et al. 2023

Chemistry & Biodiversity

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“…To examine and measure the oral organs at comparable stages of development, we selected one hundred buds from each species, sourced from 20 different plants (Mahmoudi et al 2020). The stamens and ovary were meticulously extracted from each bud and examined under a Leica MS5 stereomicroscope, with images of the oral organs captured using a Leica digital camera.…”

Section: Floral Measurementsmentioning

confidence: 99%

“…The pistil length, stigma height, style height, ovary height, ovary widh, stamen height, lament length, anther height, anther widh, pollen diameter, pollen pore, pore number, exine thickess and seed size were measured using image acquisition and analysis software (Zeiss Zen Starter). Speci cs pollen morphology were determined based on measurements of 20 grains (Mahmoudi et al 2020). To analyze individual ower traits, we conducted one-way analysis of variance (ANOVA) with sequential Bonferroni correction.…”

Section: Floral Measurementsmentioning

confidence: 99%

Comparative Floral and Seed Anatomy of Newly Identified Sarcocornia and Salicornia Species in Tunisia: Insights into Anther Development, Megasporogenesis, and Embryological Characteristics

Hayder,

Boughalleb,

Abdellaoui

et al. 2023

Preprint

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Background Detailed studies were conducted for the first time on the floral anatomical features, anther development, megasporogenesis, embryological stages, and seed anatomical characteristics of S. fruticosa, S. alpini, and S. emerici. These species were recently identified in Tunisia through a molecular study.Methods Histological sections, observations using a light microscope, and photographs were taken.Results The results indicate that the pollen grains are characterized by a pantoporate structure of predominantly spheroidal shape. The ovary takes on an annular shape and then curves to produce a campylotropic ovule. After fertilization, the nucellus gives rise to the perisperm, which is absent in mature seeds. The mature embryo exhibits a horseshoe shape and occupies almost the entire seed. The filament length, the size of the anther, pollen, ovary, style, stigma, and seed were the most developed in S. fruticosa. The radicle is longer than the cotyledons in S. emerici, whereas they are of the same length in the other two species. Regarding seed ornamentation, S. fruticosa exhibits a verrucate exotesta with short hairs and papillae that have a blunt tip. However, in S. alpini, it shows a higher density of papillae with a round and conical apex. In S. emerici, the seed surface is covered with numerous long hairs.Conclusion To our knowledge, this is the first contribution, as these species had not been previously examined. Furthermore, the findings related to megasporogenesis, female gametophyte, and embryonic development provide evidence that further studies are required for a better anatomical characterization of the Salicornia/Sarcocornia genus.

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“…Polygonum L. is a member of the Polygonaceae family with almost 150 known species distributed worldwide (Mahmoudi et al, 2020). Eight Polygonum species are endemic to Turkey (Keskin, 2012).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, anti-acetylcholinesterase, and anticancer activities of four Polygonum species from Istanbul

Yilmaz-Ozden¹,

Nasabi²,

Hasbal-Celikok³

et al. 2021

IFRJ

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Polygonum species are used in traditional medicine in many countries; some are also consumed as vegetables in Turkey. The ethanolic, methanolic, and chloroform extracts of four Polygonum species growing in Istanbul, namely P. aviculare, P. patulum subsp. pulchellum, P. lapathifolium, and the only endemic species P. istanbulicum were evaluated for their antioxidant, anti-acetylcholinesterase (AChE), and anticancer potentials. The total phenolic and flavonoid contents of the extracts were determined by Folin-Ciocalteu and aluminium chloride methods, respectively. The antioxidant capacities of the extracts were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric-reducing antioxidant power (FRAP) assays. The AChE inhibitory activities of the extracts were determined using the Ellman method. Each extract was screened for cytotoxic activity against NRK-52E and HeLa cervical cancer cell lines using the MTT assay. Among the extracts screened, ethanolic extract of P. istanbulicum showed the highest total phenolic (207.03 ± 14.12 mg GAE/g extract) and total flavonoid (124.95 ± 7.84 mg CE/g extract) contents, and antioxidant activity (DPPH EC50, 8.09 ± 0.50 mg/mL). The chloroform extract of P. lapathifolium exhibited the lowest total phenolic (22.33 ± 3.05 mg GAE/g extract) and total flavonoid (11.66 ± 0.36 mg CE/g extract) contents, and antioxidant activity (DPPH EC50, 218.44 ± 24.46 mg/mL). The extracts exhibited AChE inhibitory activity in a dose-dependent manner, particularly the ethanolic extract of P. istanbulicum which displayed strongest inhibition against AChE (88.2 ± 3.44%). AChE inhibition was minimal (32.19 ± 2.09 to 48.34 ± 3.41%) in the chloroform extracts. All ethanolic extracts revealed cytotoxic activity toward HeLa cells, while they were not cytotoxic toward NRK-52E cells. The ethanolic extract of P. lapathifolium showed the most potent cytotoxicity against HeLa cells (IC50, 8.70 ± 1.35 µg/mL). Results suggested that ethanol was the best solvent for extracting the phenolic, antioxidant, and anti-AChE compounds, and P. istanbulicum may be a potential source of these compounds. Further investigations are nevertheless required to identify the bioactive compounds present in Polygonum species.

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Flower, seed, and fruit development in three Tunisian species of Polygonum: Implications for their taxonomy and evolution of distyly in Polygonaceae

Abstract: Laboratoire des é cosystèmes pastoraux et valorisation des plantes spontanées et des microorganismes associé s, Institut des Régions Arides, Mé denine, Tunisia,

Cited by 12 publications

References 49 publications

Phenolic Profiling, Antioxidant, and Antibacterial Activities of Selected Medicinal Plants from Tunisia

Phenolic Profiling, Antioxidant, and Antibacterial Activities of Selected Medicinal Plants from Tunisia

Comparative Floral and Seed Anatomy of Newly Identified Sarcocornia and Salicornia Species in Tunisia: Insights into Anther Development, Megasporogenesis, and Embryological Characteristics

Antioxidant, anti-acetylcholinesterase, and anticancer activities of four Polygonum species from Istanbul

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