Flowering time control in ornamental gloxinia (Sinningia speciosa) by manipulation of miR159 expression

Plants that undergo tissue culture processes often change their morphology and behavior due to the "rejuvenation" caused by the plant growth regulators included in the medium. To evaluate the effects of rejuvenation by tissue culture in Prunus sp., the expression patterns of the aging gene pathway described in plant models, which include the highly conserved microRNA (miRNA or miR) 156/157 and 172 families and several of their respective target genes, were analyzed in distinct Prunus sp. genotypes at different phases of maturity, including true seedling and tissue culture micropropagated plants. In genotypes from true seedling plants, the expression of miR156 and miR157 was higher in the leaves of juvenile plants (one year old) than in those of adult plants (six year old). The opposite pattern was observed with miRNA172 expression. Our results suggest that the aging gene pathway is relatively conserved in Prunus and likely plays a key role in vegetative phase change. However, Prunus sp. plants that were rejuvenated and propagated by in vitro methods showed more erratic behavior for miR156 and miR157 and their target genes, suggesting that tissue culture alters the normal control of the aging pathway.

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“…However, we do not rule out that other miRNA families also have roles in developmental timing (Li et al, 2013;Xu et al, 2014).…”

Section: Discussionmentioning

confidence: 76%

AGING GENE PATHWAY OF MICRORNAS 156/157 AND 172 IS ALTERED IN JUVENILE AND ADULT PLANTS FROM IN VITRO PROPAGATED Prunus sp.

Bastías

Almada²,

Rojas

et al. 2016

Cienc. Inv. Agr.

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“…In studies of the molecular mechanisms of tree aging, many age-related genes have been identified (Busov et al 2004;Carlsbecker et al 2004;Diego et al 2004;Fernández-Ocaña et al 2010;García-López et al 2014;Hutchison et al 1990;Li et al 2010Xu et al 2016;Zhang et al 2016), such as miR156 (Wang et al 2011b) and Flowering Locus T (Böhlenius et al 2006;Hsu et al 2006Hsu et al , 2011, and some have been used to change the agronomic traits of plants (Flachowsky et al 2012;Kotoda et al 2006;Li et al 2013a;Yamagishi et al 2014). Larix kaempferi is a forest tree of important ecological and economic value, widely grown in the Northern Hemisphere, but its genome has not been sequenced.…”

Section: Communicated By F Gugerlimentioning

confidence: 99%

Transcriptome analysis provides insights into wood formation during larch tree aging

Yang

Zhang

et al. 2017

Tree Genetics & Genomes

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Tree age affects wood formation and yield. However, the underlying mechanisms are poorly understood, particularly at the molecular level. In this study, we investigated the transcriptomic changes of the uppermost main stems of Larix kaempferi in an entire rotation period using the RNA-Seq method. In total, ∼151 million reads were obtained from the stems of 1-, 2-, 5-, 10-, 25-, and 50-year-old L. kaempferi trees. Combining these with the published Illumina sequencing reads, 299,637 assembled transcripts were generated, of which 161,232 were annotated. Time series expression profiling identified 12,927 transcripts as differentially expressed genes (DEGs); function enrichment analysis of these DEGs showed that 459 gene ontology terms in the biological process category were enriched. These terms were associated with the processes of wood formation, such as cell differentiation, growth and death, and its hormonal regulation. Based on the expression patterns of L. kaempferi homologues of genes associated with ethylene, calcium, and cell wall expansion and synthesis, the regulatory network of tracheid growth was outlined. Altogether, the comparative transcriptomic analysis reported here demonstrated the molecular aspects of aging effects on L. kaempferi wood formation. The identification of genes involved in the regulatory network of tracheid growth provides a means of investigating the regulation of wood formation in gymnosperm trees and also offers potential targets for genetic manipulation to improve the properties of xylem fibers.

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“…Experimental studies show that miR 159 directly cleaves mRNAs encoding GAMYB‐related proteins and further controls floral initiate and another development (Achard et al, ; Li et al, ). Transgenic plants with overexpressed miR159 levels significantly delay in floral initiation and flowering under short‐day photoperiods (Achard et al, ; Li et al, ). In contrast, inhibited miRNA expression caused early flowering (Li et al, ).…”

Section: Mirna‐based Biotechnology For Improving Plant Development Fmentioning

confidence: 99%

“…Transgenic plants with overexpressed miR159 levels significantly delay in floral initiation and flowering under short‐day photoperiods (Achard et al, ; Li et al, ). In contrast, inhibited miRNA expression caused early flowering (Li et al, ). miR 172 not only regulate flower development but also involve in floral meristem initiation and phage change; this process is achieved through the interaction of miR 172 and its target Apetala 2 (AP2) that regulates the expression of SOC1, LFY and AP1 (Aukerman and Sakai, ; Chen et al, ; Mlotshwa et al, ; Glazinska et al, ; Zhu et al, ; Wollmann et al, ; Yant et al, ; Zhu and Helliwell, ; Varkonyi‐Gasic et al, ).…”

Section: Mirna‐based Biotechnology For Improving Plant Development Fmentioning

confidence: 99%

MicroRNA‐Based Biotechnology for Plant Improvement

Zhang

Wang

2014

Journal Cellular Physiology

204

123

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MicroRNAs (miRNAs) are an extensive class of newly discovered endogenous small RNAs, which negatively regulate gene expression at the post-transcription levels. As the application of next-generation deep sequencing and advanced bioinformatics, the miRNA-related study has been expended to non-model plant species and the number of identified miRNAs has dramatically increased in the past years. miRNAs play a critical role in almost all biological and metabolic processes, and provide a unique strategy for plant improvement. Here, we first briefly review the discovery, history, and biogenesis of miRNAs, then focus more on the application of miRNAs on plant breeding and the future directions. Increased plant biomass through controlling plant development and phase change has been one achievement for miRNA-based biotechnology; plant tolerance to abiotic and biotic stress was also significantly enhanced by regulating the expression of an individual miRNA. Both endogenous and artificial miRNAs may serve as important tools for plant improvement.

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Flowering time control in ornamental gloxinia (Sinningia speciosa) by manipulation of miR159 expression

Abstract: miR159-regulated GAMYB expression is an effective pathway of flowering time control in gloxinia. Transgenic manipulation of miR159 can be used as an applicable strategy to regulate flowering time in commercial ornamental plants.

Cited by 87 publications

References 33 publications

AGING GENE PATHWAY OF MICRORNAS 156/157 AND 172 IS ALTERED IN JUVENILE AND ADULT PLANTS FROM IN VITRO PROPAGATED Prunus sp.

AGING GENE PATHWAY OF MICRORNAS 156/157 AND 172 IS ALTERED IN JUVENILE AND ADULT PLANTS FROM IN VITRO PROPAGATED Prunus sp.

Transcriptome analysis provides insights into wood formation during larch tree aging

MicroRNA‐Based Biotechnology for Plant Improvement

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