Fluid dynamic analysis of flow in orbiting dishes and the effects of flow on shear stress and endothelial cellular responses.

Chakraborty, Amlan

doi:10.18297/etd/230

Cited by 2 publications

(2 citation statements)

References 29 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stem cell is a complex microenvironment combining an extracellular matrix, cell-to-cell interactions, and other factors such as growth factors, physical factors, and various cytokines. Stem cells are exposed to high Ca 2+ concentrations and a variety of autocrine, paracrine, and endocrine signals (extrinsic factors) and they are attached to the ECM through integrin receptors [ 1 – 10 ]. Many researchers have already reported an influence of cell growth and differentiation with the use of physical stimulators.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effects of Orbital Shear Stress onIn VitroGrowth and Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells

Lim¹,

Hexiu

Kim

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

Cellular behavior is dependent on a variety of physical cues required for normal tissue function. In order to mimic native tissue environments, human alveolar bone-derived mesenchymal stem cells (hABMSCs) were exposed to orbital shear stress (OSS) in a low-speed orbital shaker. The synergistic effects of OSS on proliferation and differentiation of hABMSCs were investigated. In particular, we induced the osteoblastic differentiation of hABMSCs cultured in the absence of OM by exposing hABMSCs to OSS (0.86–1.51 dyne/cm2). Activation of Cx43 was associated with exposure of hABMSCs to OSS. The viability of cells stimulated for 10, 30, 60, 120, and 180 min/day increased by approximately 10% compared with that of control. The OSS groups with stimulation of 10, 30, and 60 min/day had more intense mineralized nodules compared with the control group. In quantification of vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2) protein, VEGF protein levels under stimulation for 10, 60, and 180 min/day and BMP-2 levels under stimulation for 60, 120, and 180 min/day were significantly different compared with those of the control. In conclusion, the results indicated that exposing hABMSCs to OSS enhanced their differentiation and maturation.

show abstract

Section: Introductionmentioning

confidence: 99%

Synergistic Effects of Orbital Shear Stress onIn VitroGrowth and Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells

Lim¹,

Hexiu

Kim

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…The data show that less oscillation between the two primary directions occurred near the center of the narrow channel gap. In past experiments the DOSI values varied with radial location differently (Charkraborty et al (2011)). In that study, the normal trend increased from ~0 close to the 0% radial location to ~ 1 close to the 100% radial location.…”

Section: E Ncf Dosi Vs Locationmentioning

confidence: 99%

DOSI in novel geometry dishes

Jones

View full text Add to dashboard Cite

Fluid dynamic analysis of flow in orbiting dishes and the effects of flow on shear stress and endothelial cellular responses.

Cited by 2 publications

References 29 publications

Synergistic Effects of Orbital Shear Stress onIn VitroGrowth and Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells

Synergistic Effects of Orbital Shear Stress onIn VitroGrowth and Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells

DOSI in novel geometry dishes

Contact Info

Product

Resources

About