Fluid Formed by Choroid Plexus: A Technique for Its Collection and a Comparison of Its Electrolyte Composition With Serum and Cisternal Fluids

The formation of cerebrospinal fluid: Nearly a hundred years of interpretations and misinterpretations

“…The third reason why the choroid plexuses are considered to the main site of formation should be the fact that the observations mentioned so far were accompanied by a significant number of concurrent studies that pointed to CSF formation by the choroid plexuses (Dandy, 1919;de Rougemont et al, 1960;Pollay et al, 1972;Weed, 1914;Welch, 1963) , and the secretory character of the choroid plexuses was confirmed by the application of the molecular biological methods during the past two decades (Brown et al, 2004;Johanson, 2008;Kanaka et al, 2001;Li et al, 2002;Oshio et al, 2005;Praetorius et al, 2004;Speake et al, 2003;Watts et al, 1991;Zlokovic et al, 1993).…”

“…Choroid plexuses are the main site of CSF production (Dandy, 1919;Dandy and Blackfan, 1914;Davson, 1967;Davson et al, 1987;de Rougemont et al, 1960;Johanson et al, 2008;Masserman, 1934;Milhorat, 1987;Netsky and Shuangshoti, 1970;Rubin et al, 1966;Spector and Johanson, 1989;Welch, 1963;Welch, 1975), and other extrachoroidal sources also participate in maintaining the balance (Milhorat, 1987). According to Pollay and Curl (1967), another source of CSF is the ependyma lining the ventricles (Pollay and Curl, 1967).…”

“…Collection of new CSF on the choroid plexus surface (de Rougemont et al, 1960) was performed in cats by surgical access to the brain ventricles and by lowering a glass pipette to make contact with the plexus which had been covered with pantopaque oil. Under such circumstances, the new CSF enters the glass capillary and may be collected and measured, which demonstrated the role of choroid plexuses in the secretion of CSF (ml/min).…”

Relation of potassium concentration in choroidplexus fluid to that in plasma.

“…1955). It probably also takes place in the normal course of CSF circulation (de Rougemont et al 1960). The transport processes whose existence may be inferred from these observations might be expected to provide additional regulation of CSF K+ concentration.…”

“…Choroid-plexus fluid was sampled by a procedure which has been described in detail elsewhere (de Rougemont et al 1960). In brief, the skull was removed on one side, and the cerebral hemisphere was gently retracted laterally to expose the corpus callosum, which was separated by blunt dissection to unroof the lateral ventricle.…”