Fluidized-bed bioartificial liver assist devices (BLADs) based on microencapsulated primary porcine hepatocytes have risk of porcine endogenous retroviruses transmission

Yang, Qian; Liu, Fei; Pan, Xiao; Lv, Guoliang; Zhang, AnYe; Yu, Chen Bo; Li, Lanjuan

doi:10.1007/s12072-010-9210-6

Cited by 19 publications

(11 citation statements)

References 27 publications

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“…Various efforts have been made to maintain the functional performance of hepatocytes by providing proper extracellular matrix for attachment either as scaffolds , microcarriers or cell entrapment/encapsulation devices . The main concerns in using xenogenic porcine hepatocytes is the transfer of zoonotic diseases , protein–protein incompatibility and the possible immune responses generated during treatment . There is therefore a compelling need to seek alternative cell sources.…”

Section: Cell Sources For Liver Tissue Engineeringmentioning

confidence: 99%

Liver tissue engineering and cell sources: issues and challenges

Palakkan

Hay

et al. 2013

Liver International

112

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Liver diseases are of major concern as they now account for millions of deaths annually. As a result of the increased incidence of liver disease, many patients die on the transplant waiting list, before a donor organ becomes available. To meet the huge demand for donor liver, alternative approaches using liver tissue engineering principles are being actively pursued. Even though adult hepatocytes, the primary cells of the liver are most preferred for tissue engineering of liver, their limited availability, isolation from diseased organs, lack of in vitro propagation and deterioration of function acts as a major drawback to their use. Various approaches have been taken to prevent the functional deterioration of hepatocytes including the provision of an adequate extracellular matrix and co-culture with non-parenchymal cells of liver. Great progress has also been made to differentiate human stem cells to hepatocytes and to use them for liver tissue engineering applications. This review provides an overview of recent challenges, issues and cell sources with regard to liver tissue engineering.

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Section: Cell Sources For Liver Tissue Engineeringmentioning

confidence: 99%

Liver tissue engineering and cell sources: issues and challenges

Palakkan

Hay

et al. 2013

Liver International

112

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“…In order to protect cells from toxic exposures to perfused blood, microencapsulated hepatocytes have been used in fluidized bed-type bioreactors for BAL support recently [51,52]. The advantage of fluidized bed-type bioreactors is the large mass transfer area provided by spherical capsules that contain the cells.…”

Section: Development Of Bal Devicesmentioning

confidence: 99%

Liver Regeneration

Kim

Özer

Uygun

2014

Regenerative Medicine Applications in Organ Transplantation

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“…VIRIONS BY PORCINE CELLS RNA expression of PERV has been found in several types of tissue, including kidney, lung, skin, liver, and pancreatic islands (Le Tissier et al, 1997;Patience et al, 1997;Akiyoshi et al, 1998;Martin et al, 1998a;van der Laan et al, 2000;Yang et al, 2010). Neverthe less, expression of viral RNA cannot be correlated with the isolation of infection particles.…”

Section: Rna Expression and Release Of Pervmentioning

confidence: 99%

Porcine endogenous retroviruses: What are the risks of infection transmission in xenotransplantation?

Yudin

Aitnazarov

Ermolaev

2011

Russ J Genet Appl Res

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Modern studies on porcine endogenous retroviruses are considered in the review. Data on the nucleotide sequences of retroviruses, their expression, and the isolation of mature virions is discussed. The tropism of endogenous retroviruses to human cells and retrospective studies of patients are considered in detail. A critical overview of works on in vitro and in vivo interspecific transmission of porcine endogenous retroviruses is presented. Tactics for preventing possible infection and treating humans for it are discussed. Based on the overviewed data, it is concluded that the risk of infection transmission from xenograft recipients to the rest of the population is very low. This low risk can be further reduced by careful observation and, prob ably, vaccination of contacting persons, as well as by preventive use of antiviral drugs. The minor risk of infec tion transmission can also be reduced by application of modern biotechnological methods to raise pigs that have no endogenous viruses tropic to humans.

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Fluidized-bed bioartificial liver assist devices (BLADs) based on microencapsulated primary porcine hepatocytes have risk of porcine endogenous retroviruses transmission

Abstract: The kind of fluidized-bed BLADs based on microencapsulated primary porcine hepatocytes have risk of PERVs transmission. Further extensive pre-clinical study focused on biosafety is warranted.

Cited by 19 publications

References 27 publications

Liver tissue engineering and cell sources: issues and challenges

Liver tissue engineering and cell sources: issues and challenges

Liver Regeneration

Porcine endogenous retroviruses: What are the risks of infection transmission in xenotransplantation?

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