Fluorescamine Use in High-Performance Liquid Chromatographic Determination of Aminocaproic Acid in Serum

Farid, Nagy A.

doi:10.1002/jps.2600680237

Cited by 21 publications

(7 citation statements)

References 17 publications

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“…Pregabalin concentrations were measured via quantitative assay performed by a commercial laboratory 3 using an HPLC procedure modified from a previously described method. This assay has been shown to have a lower limit of detection of 0.10 μg/mL, with a between-run precision of 12.9, 2.6, and 1.9% at concentrations of 0.1, 2.0 and 8.0 μg/mL ( 16 ).…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of Single-Dose Oral Pregabalin Administration in Normal Cats

et al. 2018

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Objective: To describe the pharmacokinetic parameters of oral pregabalin in normal cats after single oral dosing.Animals: Six healthy adult research cats.Procedures: Following sedation and indwelling catheter placement, one oral (4 mg/kg) dose of pregabalin was administered. Blood samples were collected at 0, 15 and 30 min and 1, 1.5, 2, 3, 4, 6, 8, 12, 24, and 36 h after administration. Plasma pregabalin concentrations were measured by high-performance liquid chromatography and subjected to pharmacokinetic analysis using commercial software.Results: Four of six cats developed moderate sedation after pregabalin administration. The peak pregabalin concentration was 8.3 ± 1.6 μg/ml which occurred at 2.9 ± 1.2 h. Elimination half-life was 10.4 ± 2.6 h and area under the curve was 133.9 ± 71.5 μg-h/ml. Time above the minimum therapeutic concentration for seizure control in dogs and people (2.8 μg/ml) was 17.6 ± 6.2 h. Using these data, predicted minimum, maximum and average steady state concentrations were calculated for 12 and 24 h dosing intervals.Conclusions and Clinical Relevance: Pregabalin (4 mg/kg) administered orally to cats results in plasma concentrations within the range considered to be efficacious for seizure control in dogs and humans between 1.5 and at least 12 h. Because of moderate sedative side effects in the majority of cats at this dose and high calculated maximum steady state concentrations, a lower dose, given more frequently (1–2 mg/kg q 12 h), should be evaluated in prospective clinical studies.

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Section: Methodsmentioning

confidence: 99%

Pharmacokinetics of Single-Dose Oral Pregabalin Administration in Normal Cats

et al. 2018

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“…In the case of a presence of other reacting compounds in the mixture, classical chromatographic or HPLC methods of separation are used (46)(47)(48)(49). Antifibrinolytic activity of EACA is determined after a deproteinization of plasma, urine and tissue homogenate with the use of trichloroacetic acid (TCA) or perchloric acid (HClO 4 ) (50,51).…”

Section: Determination Of Concentration and Activitymentioning

confidence: 99%

ε-Aminocaproic Acid (EACA)

Gacko

Worowska

2008

Polish Journal of Surgery

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“…Residual levels of dextran were assessed using the Anthrone assay [27], and the residual contents of Amicar were determined fluorometrically [28] in three consecutive plasmin preparations, Plasminogen yields from the cartridge were quantified by means of a bicinchoninic acid (BCA) assay (Pierce, Rockford, IL, USA) to determine the appropriate amount of streptokinase (Streptase, Behringwerke, Marburg, Germany) to add to generate a ratio of 1:10 between streptokinase and plasmin. Plasmin activities were determined with a chromogenic assay with D-Val-Leu-Lys-pNA (S2251, Sigma-Aldrich, V0882) [29,30], which was standardized against the 3rd International Reference Preparation for Plasmin (National Institute for Biological Standards and Control, Potters Bar, UK) and expressed in International Units (IU).…”

Section: Definition and Activity Testing Of Plasmin Isolatesmentioning

confidence: 99%