Fluorescein Dyes

Ziarani, Ghodsi Mohammadi; Moradi, Razieh; Lashgari, Negar; Kruger, Hendrik G.

doi:10.1016/b978-0-12-815647-6.00010-8

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…Carbazoles have a tricyclic structure, consisting of two six-membered benzene rings fused on each side of a ring. One side of a ring contains five-membered nitrogen [155]. Examples of this group are carquinostatin, koenimbin, and koenigicine, among others.…”

Section: Alkaloids From Mushroomsmentioning

confidence: 99%

Bioactive Compounds Produced by Macromycetes for Application in the Pharmaceutical Sector: Patents and Products

Martinez-Burgos,

Montes Montes,

Pozzan

et al. 2024

Fermentation

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It is widely known that mushrooms present several properties with applications in the medicinal and pharmaceutical sectors, including antimicrobial, immunomodulatory, antioxidant, hypotensive, neuroprotective, and anti-inflammatory activities. This article aims to review examples of the bioactive metabolites responsible for those activities, such as polysaccharides, phenols and polyphenols, terpenes, peptides, alkaloids, and steroids, which are produced by several relevant mushroom species. It also discusses their production through solid-state fermentation and submerged fermentation, as well as the processes of obtention of mushroom bioactive extracts and considerations on their stability aiming industrial applications. In addition, the article examines the patent landscape surrounding mushroom-derived bioactives, shedding light on the intellectual property history and innovations driving this field forward. Examples of recently deposited patents in the field are highlighted, as well as the main depositors. China and the United States are the major depositor countries in this field (52% and 35% of patents, respectively), and the principal compounds on the patents are polysaccharides and alkaloids. The article also provides insights into the current market landscape, showcasing mushroom-derived products in the pharmaceutical field available to consumers. From dietary supplements to skincare formulations, the market offerings reflect the growing interest in harnessing the health benefits of mushroom bioactives.

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Section: Alkaloids From Mushroomsmentioning

confidence: 99%

Bioactive Compounds Produced by Macromycetes for Application in the Pharmaceutical Sector: Patents and Products

Martinez-Burgos,

Montes Montes,

Pozzan

et al. 2024

Fermentation

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“…It provided a site of commonality while other changes were made across the compound series. 6-Carboxyfluorescein (λex/em 492/517 nm) was used as the fluorophore due to its biocompatibility, high extinction coefficient (70,000 M −1 cm −1 ) and high fluorescence quantum yield (ϕ = 0.85) [ 14 , 15 ] with the building block, Fmoc-Lys(6-Carboxyfluorescein-diacetate)-OH, developed to allow an efficient/clean probe synthesis. Carboxyfluorescein was also chosen, rather than rhodamine-based dyes, as it is negatively charged and as such known not to be cell-permeable.…”

Section: Introductionmentioning

confidence: 99%

Solid-Phase Synthesis of Fluorescent Probes for Plasma Membrane Labelling

2021

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The cellular plasma membrane plays a fundamental role in biological processes, including cell growth, signaling and transport. The labelling of the plasma membrane with targeted fluorescent probes offers a convenient and non-invasive way to image the morphological changes and dynamics of a membrane in real-time and, despite many examples of fluorescent plasma membrane probes, a “universal targeting/anchoring moiety” is still required. In this study, a small number of stearic acid-based probes labelled with 6-carboxyfluorescein was designed and fabricated via solid-phase synthesis in which variations in both charge and hydrophobicity were explored. To ease the synthesis process, a gram-scale synthesis of the Fmoc-Lys(6-carboxyfluoresein diacetate)-OH building block was developed, allowing the discovery of optimal probes that carried a positively charged amino group and a stearic acid tail that exhibited intense plasma membrane brightness and robust retention.

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“…67-69 Fluorescein is a xanthene dye that has been widely used as a fluorescent label in biological applications because of its strong fluorescence. 70 In 2005, Noble and co-workers determined the effect of the nucleobase overhangs on the complementary strand on the fluorescent signal of the hybridization fluoresceinlabelled probe (Figure 1.15). 71 The results showed the presence of guanine base overhangs in the close proximity to the fluorescein resulted in the highest fluorescence quenching.…”

Section: Guanine Rich Sequence Dna As a Quenchermentioning

confidence: 99%

Interactions of pyrrolidinyl peptide nucleic acid with oligodeoxyguanosine and biosensing applications

Charoenpakdee

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Pyrrolidinyl peptide nucleic acid is a DNA mimic consisting of proline-2-aminocyclopentane carboxylic acid (acpcPNA) as a core structure. It binds to DNA to form a more stable hybrid while showing greater selectivity and affinity than the conventional DNA and PNA probes. In this study, we aim to develop novel DNA sensing systems based on the acpcPNA probe. In the first part, graphene oxide (GO) was used as an external quencher which could effectively quench the fluorescein-labeled PNA probe. A remarkable fluorescence restoration was achieved after the addition of a complementary DNA target. This acpcPNA-GO combination was successfully used to monitor the strand-invasion of the duplex DNA by acpcPNA, and to detect two DNA targets in a multiplex, multicolor detection. In the second part, we demonstrated the effective quenching of fluorescently labeled acpcPNA by using oligodeoxyguanosine as an external quencher. The electrostatic interaction between the positively-charged modification on the PNA probe and the phosphate backbone of the oligodeoxyguanosine (dGX) resulted in a strong association, resulting in an effective quenching of the fluorescence by photoinduced electron transfer. The addition of the complementary DNA strand rapidly and completely restored the fluorescence without detaching the dGX from the PNA probe. This sensing system could detect the complementary DNA target with single-base mismatch specificity and could be applied to detect various other DNA sequences. The acpcPNA-dGX platform has been used in the multiplex detection of two DNA sequences and to detect Hg?? ion as well as to monitor the PNA ligation. In the last part, a new DNA sensing system based on the combination of acpcPNA-dGX in the context of DNAzyme was explored. Preliminary studies revealed a promising result from the colorimetric DNA detection employing a strand displacement probe strategy. In short, binding of the dGX-modified DNA strand to PNA diminished its peroxidase activity, which could be restored when the DNA target was added to displace the DNAzyme.

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Fluorescein Dyes

Cited by 6 publications

References 23 publications

Bioactive Compounds Produced by Macromycetes for Application in the Pharmaceutical Sector: Patents and Products

Bioactive Compounds Produced by Macromycetes for Application in the Pharmaceutical Sector: Patents and Products

Solid-Phase Synthesis of Fluorescent Probes for Plasma Membrane Labelling

Interactions of pyrrolidinyl peptide nucleic acid with oligodeoxyguanosine and biosensing applications

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