Fluorescence aggregation assay for the protein biomarker mucin 1 using carbon dot-labeled antibodies and aptamers

Ma, Nan; Jiang, Wentao; Li, Ting; Zhang, Zhongqiang; Qi, Haiyun; Yang, Minghui

doi:10.1007/s00604-014-1386-3

Cited by 50 publications

(20 citation statements)

References 26 publications

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Section: Sensing and Imaging Applicationsmentioning

confidence: 99%

“…C dots have been used for quantitation of analytes in more complicated biological samples 8b,14d,84b,104. The C dots prepared from p ‐aminobenzoic acid in ethanol were used for the detection of hematin in human red blood cells based on the inner filter effect.…”

Section: Sensing and Imaging Applicationsmentioning

confidence: 99%

“…The C dots prepared from p ‐aminobenzoic acid in ethanol were used for the detection of hematin in human red blood cells based on the inner filter effect. Hematin with a high molar extinction coefficient (ε = 54 200 × 10 −6 m L −1 cm −1 ) at 386 nm can induce high inner filter effect efficiency of the C dots, leading to high sensitivity for hematin (LOD of 0.25 × 10 −6 m with a linear concentration range of 0.5–10 × 10 −6 m ) 104b. Based on the inner filter effect, cytochrome c that is an important biomarker for early‐stage apoptosis with an absorption peak at 408 nm was determined using nitrogen‐doped C dots 84b.…”

Section: Sensing and Imaging Applicationsmentioning

confidence: 99%

“…The assay was further validated with the analyses of Pearl River water, tea, and soil samples, with good recovery. An important human cancer biomarker, mucin 1 protein (MUC1) was detected using antibody and aptamer conjugated C dots as shown in Figure c 104a. Antibody and amino terminated aptamer against MUC1 were used separately to functionalize C dots prepared from glucose.…”

Section: Sensing and Imaging Applicationsmentioning

confidence: 99%

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Recent Advances and Sensing Applications of Carbon Dots

2019

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Carbon dots (C dots) with biocompatibility, brightness, stability against photoirradiation and salt, and ease in preparation have become important materials for sensing and imaging. They can be prepared from natural materials and small organic molecules through hydrothermal, microwave‐assistant, and electrochemical methods, with advantages of simplicity and low cost. To enhance the quantum yields of C dots in the red and near‐infrared regions, doping of C dots with heteroatoms such as nitrogen and sulfur has been suggested. C dots both with and without being functionalized recognition elements such as antibodies and aptamers are selective and sensitive for sensing of analytes, including metal ions (e.g., Fe3+, Hg2+, Cu2+), small molecules (e.g., H2O2, cysteine, glutathione), and biopolymers like proteins, as well as for in vitro and in vivo imaging. Depending on the size, charge, and surface ligands of C dots used to label cells, fluorescence images of different organelles are shown. Multicolor images of bacteria, mammalian cells, and plant tissues incubated with C dots are realized when excited at different wavelengths. In this review, many excellent sensing and imaging examples of C dots are presented to highlight their features and to show their challenges for analytical applications.

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Section: Sensing and Imaging Applicationsmentioning

confidence: 99%

Section: Sensing and Imaging Applicationsmentioning

confidence: 99%

Section: Sensing and Imaging Applicationsmentioning

confidence: 99%

Section: Sensing and Imaging Applicationsmentioning

confidence: 99%

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Recent Advances and Sensing Applications of Carbon Dots

2019

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“…Among them, uorescence assay is a well-known detection method with acknowledged advantages such as high sensitivity, easy readout, simplicity and the feasibility of quantication. 22,24 Despite many advances in this eld, these strategies still have shortcomings including the costly modication of uorophores and potential false positive signals. Moreover, the sensitivity needs to be further improved.…”

Section: Introductionmentioning

confidence: 99%