Fluorescence amplification using photonic crystal slabs

Enhanced fluorescence detection of miRNA-16 on a photonic crystal

“…As a result, the Limit of Detection (LoD) is demonstrated to decrease by a factor of about 50. [18][19][20][21] In this work, we demonstrate how a properly functionalized 22 all-dielectric structure is able to enhance the sensitivity in a fluorescence-based detection of labelled miRNA, resulting in a decrease of the LoD by a factor of about 50. 1 They have been widely studied over the past few decades as promising biomarkers, for both diagnostic and therapeutic applications, 2 because they could be over-or under-expressed in different pathological responses such as diabetes, 3 neurodegenerative disorders 4 and various cancers.…”

“…5 Detecting miRNAs is one of the new challenges of analytical technologies. [18][19][20][21] In this work, we demonstrate how a properly functionalized 22 all-dielectric structure is able to enhance the sensitivity in a fluorescence-based detection of labelled miRNA, resulting in a decrease of the LoD by a factor of about 50. In an attempt to increase the specificity of recognition while decreasing the minimum detectable amount of miRNA (Limit of Detection, LoD), several detection approaches have been proposed in the past.…”

“…In an attempt to increase the specificity of recognition while decreasing the minimum detectable amount of miRNA (Limit of Detection, LoD), several detection approaches have been proposed in the past. [18][19][20][21] In this work, we demonstrate how a properly functionalized 22 all-dielectric structure is able to enhance the sensitivity in a fluorescence-based detection of labelled miRNA, resulting in a decrease of the LoD by a factor of about 50. reverse transcriptase polymerase chain reaction RT-PCR) and pre-concentration protocols (e.g.…”