Fluorescence-based determination of the copper concentration in drinking water

Hötzer, Benjamin; Scheu, Timo; Jung, Gregor; Castritius, Stefan

doi:10.1117/1.oe.52.5.053602

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Cited by 4 publications

(1 citation statement)

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“…GFP with the His 6 -tag exhibited a significant decrease in fluorescence lifetime in the presence of Cu 2+ ions, while displayed minimal or no response to other metal ions. 85,86 The high selectivity for Cu 2+ allowed GFP and its variants T203V, eYFP, and S65T to act as copper ion indicators. 85 It is possible that other color FPs with the His 6 -tag have a similar fluorescence lifetime behavior for Cu 2+ .…”

Section: Analytesmentioning

confidence: 99%

Genetically encoded fluorescence lifetime biosensors: overview, advances, and opportunities

Mo,

Zhou,

et al. 2023

Analyst

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Section: Analytesmentioning

confidence: 99%

Genetically encoded fluorescence lifetime biosensors: overview, advances, and opportunities

Mo,

Zhou,

et al. 2023

Analyst

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Live‐Cell Copper‐Induced Fluorescence Quenching of the Flavin‐Binding Fluorescent Protein CreiLOV

2020

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CreiLOV is a flavin‐binding fluorescent protein derived from the blue‐light photoreceptor protein family that contains light‐oxygen‐voltage (LOV) sensing domains. Flavin‐binding fluorescent proteins represent a promising foundation for new fluorescent reporters and biosensors that can address limitations of the well‐established green fluorescent protein (GFP) family. Flavin‐binding fluorescent proteins are smaller than GFPs, are stable over a wider pH range, offer rapid chromophore incorporation, and are oxygen‐independent so can be applied to live anaerobic organisms. Among the flavin‐binding fluorescent proteins, CreiLOV has a high quantum yield and excellent photophysical properties, making it promising for cellular applications. Here, we investigated the suitability of CreiLOV as an intensity‐ and fluorescence‐lifetime‐based metal sensor. CreiLOV selectively binds copper(II) over other biologically relevant metals with low‐micromolar affinity, resulting in fluorescence quenching and a decrease in the fluorescence lifetime that can be observed in cuvettes and live bacterial cells.

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A high-affinity fluorescence probe for copper(II) ions and its application in fluorescence lifetime correlation spectroscopy

et al. 2019

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Fluorescence-based determination of the copper concentration in drinking water

Cited by 4 publications

References 16 publications

Genetically encoded fluorescence lifetime biosensors: overview, advances, and opportunities

Genetically encoded fluorescence lifetime biosensors: overview, advances, and opportunities

Live‐Cell Copper‐Induced Fluorescence Quenching of the Flavin‐Binding Fluorescent Protein CreiLOV

A high-affinity fluorescence probe for copper(II) ions and its application in fluorescence lifetime correlation spectroscopy

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