Fluorescence-based Lab-on-Chip spot design for improved signal detection

Bassani, Thiago; Dias, P.; Branco, Gilberto; Silva, Wilson J. da; Neto, Hugo Vieira; Schneider, Fábio Kurt

doi:10.1109/embc.2012.6345988

Cited by 3 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Despite several possibilities for integrating compact fluorescence sensors on LoC systems have been developed [ 71 ], the employment of bulk optical components, like lenses or optical fibers, are still used for the coupling of the detector and the fluorescence source [ 72 ]. This external optical coupling strongly reduces the portability of the developed systems.…”

Section: Evaluation Of System Performancesmentioning

confidence: 99%

Thin-Film-Based Multifunctional System for Optical Detection and Thermal Treatment of Biological Samples

et al. 2022

View full text Add to dashboard Cite

In this work, we present a multifunctional Lab-on-Chip (LoC) platform based on hydrogenated amorphous silicon sensors suitable for a wide range of application in the fields of biochemical and food quality control analysis. The proposed system includes a LoC fabricated on a 5 cm × 5 cm glass substrate and a set of electronic boards for controlling the LoC functionalities. The presented Lab-on-Chip comprises light and temperature sensors, a thin film resistor acting as a heating source, and an optional thin film interferential filter suitable for fluorescence analysis. The developed electronics allows to control the thin film heater, a light source for fluorescence and absorption measurements, and the photosensors to acquire luminescent signals. All these modules are enclosed in a black metal box ensuring the portability of the whole platform. System performances have been evaluated in terms of sensor optical performances and thermal control achievements. For optical sensors, we have found a minimum number of detectable photons of 8 × 104 s−1·cm−2: at room temperature, 1.6 × 106 s−1·cm−2: in presence of fluorescence excitation source, and 2.4 × 106 s−1·cm−2· at 90 °C. From a thermal management point of view, we have obtained heating and cooling rates both equal to 2.2 °C/s, and a temperature sensor sensitivity of about 3 mV/°C even in presence of light. The achieved performances demonstrate the possibility to simultaneously use all integrated sensors and actuators, making promising the presented platform for a wide range of application fields.

show abstract

Section: Evaluation Of System Performancesmentioning

confidence: 99%