Fluorescence detection in automated DNA sequence analysis

Smith, Lloyd M.; Sanders, Jane Z.; Kaiser, Robert; Hughes, Peter J.; Dodd, C.E.R.; Connell, Charles R.; Heiner, Cheryl; Kent, Stephen B. H.; Hood, Leroy

doi:10.1038/321674a0

Cited by 1,526 publications

(640 citation statements)

References 6 publications

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“…Por último, los productos amplificados se purificaron y secuenciaron en ambos sentidos (13,14), empleando los mismos cebadores de la reacción original mediante el sistema de electroforesis capilar ABI 3730.…”

Section: Rt-pcr Anidada Para La Detección De Arn De Lcmv En Muestras unclassified

First evidence of Lymphocytic Choriomeningitis virus (Arenaviruses) infection in Mus musculus rodents captured in the urban area of the municipality of Sincelejo-Sucre, Colombia

et al. 2016

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Contribución de los autores:Anaís Castellar: revisión de la literatura científica, recolección y análisis de datos y redacción del manuscrito Marco Guevara y Esteban Arroyave: trabajo de campo y organización de los datos Juan D. Rodas y Andrés Londoño: trabajo de laboratorio, asesoría en la revisión y aportes a la discusión Francisco J. Díaz: revisión crítica y asesoría del contenido, aportes a la discusión y análisis filogenéticos. Silvana Levis: donación de reactivos no comerciales y asesoría en los ensayos, revisión crítica y aportes a la discusión Pedro J. Blanco: contribución en el diseño del estudio, participación en la fase de campo y revisión crítica del manuscrito Results: We found a 10% seroprevalence (8/80) and the viral genome was detected in 16 brain samples; the alignment (BLAST) and the phylogenetic analysis (MrBayes, version 3.2.2) confirmed the presence of the lymphocytic choriomeningitis virus. Conclusion:The results indicated that human infection with the lymphocytic choriomeningitis virus in humans could occur in the urban area of Sincelejo, although no cases have been reported so far.

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Section: Rt-pcr Anidada Para La Detección De Arn De Lcmv En Muestras unclassified

First evidence of Lymphocytic Choriomeningitis virus (Arenaviruses) infection in Mus musculus rodents captured in the urban area of the municipality of Sincelejo-Sucre, Colombia

et al. 2016

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“…An initial denaturation step of 15 min at 95°C was followed by 50 cycles, including at 94°C for 50 s, 55°C for 50 s , and 72°C for 1 min on a GeneAmp PCR system 9700 (Applied Biosystems, Weiterstadt, Germany). As confirmation of the specificity of the PCR results, especially of the positive B19V samples, automatic DNA sequencing of the B19V amplification products was performed [Smith et al, 1986]. Differentiation of the three B19V genotypes was performed by identification of the genotypes 1 (prototype), 2 (LaLi-like) and 3 (V9-like) as described previously [Servant et CAT GGG GTT GAG-3).…”

Section: Detection Of Parvoviral Enteroviral and Adenoviral Genomesmentioning

confidence: 99%

Low level myocardial parvovirus B19 persistence is a frequent finding in patients with heart disease but unrelated to ongoing myocardial injury

Lotze¹,

Egerer

Glück

et al. 2010

Journal of Medical Virology

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While myocardial parvovirus B19 (B19V), aside from enteroviruses (EV) and adenoviruses (ADV), has recently been found often in patients with myocarditis and idiopathic dilated cardiomyopathy (IDC), the pathogenetic significance of B19V genomes in those patients has not yet been sufficiently elucidated. In the present study, left ventricular endomyocardial biopsies from 24 patients with left ventricular ejection fraction (LVEF) below 55% due to IDC, and tissue from the right atrial appendage of 10 control patients undergoing bypass surgery with normal LVEF (>55%) were investigated for B19V, ADV, and EV genomes by specific nested polymerase chain reaction (PCR), by real time PCR or by reverse‐transcription PCR, respectively. The myocardial tissue samples from the 10 controls were analyzed each in three different virological laboratories for B19V. In the IDC group, the frequency of the myocardial virus genomes found in 54% (13/24) of the patients was as follows: B19V: 50% (12/24), EV: 8% (2/24), including one patient with B19V and EV, and ADV: 0% (0/24). For comparison, the prevalence of B19V genomes was between 30% and 60% in the control group as detected in three different laboratories, but all these control subjects were EV‐ and ADV‐negative. The number of B19V gene copies, however, was very low and similar both in the IDC and control group. In the majority of patients myocardial B19V persistence was associated with a low virus load irrespective of the underlying heart disease so that it may be of no importance in the pathogenesis of IDC. J. Med. Virol. 82:1449–1457, 2010. © 2010 Wiley‐Liss, Inc.

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“…15 Recombi-DNA sequencer (Perkin Elmer/Applied Biosystems Division, Foster City, CA, USA) using dye terminators. 18 The complete nants were packaged using Gigapack III Gold Packaging Extracts (Stratagene). A total of 1.6 × 10 7 recombinants were sequences for elf-2a and elf-2b were assembled using Genetics Computer Group (GCG) software (Madison, WI, USA).…”

Section: Isolation Of Elf-2 Clones Cell Lines and Yeastmentioning

confidence: 99%

Elf-2, a rhombotin-2 binding ets transcription factor: discovery and potential role in T cell leukemia

et al. 1997

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Rhombotin-2 (RBTN-2) is a proto-oncogene only in the context transcription factors. For example, RBTN-2 has recently been of T lymphocytes. We postulated that the oncogenic effect of shown to bind erythroid transcription factors tal-1, GATA-1, RBTN-2 in T cells is likely mediated by binding protein(s) withand GATA-2. 9 These data, considered in light of the nuclear T cell-specific expression. By screening a T cell cDNA library, location of RBTN-2 in hematopoietic cells, 7 suggest that we identified a novel ets transcription factor that binds RBTN-RBTN-2 has a direct role in transcriptional regulation of 2. This protein was named elf-2 because its DNA-binding domain is virtually identical to that of ets family member elf-1. erythroid development. RBTN-2 may also regulate other Northern analyses showed similar levels of two elf-2 transcripts genes. For example, RBTN-2 likely regulates the expression of RBTN-2 binds other proteins via its two tandem LIM domains, which make up approximately two-thirds of its mass Introduction (reviewed in Ref. 11). Each LIM domain coordinates two zinc cations, which presumably hold the intervening residues in The proto-oncogene rhombotin-2 (RBTN-2) was initially loop-like structures reminiscent of zinc finger domains. The implicated in T cell leukemogenesis by the high frequency of residues required for zinc cation-coordination and structural non-random chromosomal translocations involving the RBTNintegrity are highly conserved. The other amino acid residues, 2 locus (at 11p13) in childhood T cell acute leukemia. 1,2 and to some degree the length of the LIM domain loop porThese translocations almost invariably result in the high tions are variable, and thus confer each LIM domain its own expression of RBTN-2 in T cells where RBTN-2 is not normally specific function. LIM domains have been shown to facilitate expressed. 2,3 Recent transgenic studies have confirmed the a variety of protein-protein interactions. 9,12,13 For example, role of RBTN-2 as a proto-oncogene. Enforced expression of the LIM domains of RBTN-2 mediate its binding to tal-1 13 and RBTN-2 in T cells of transgenic mice results in T cell lym-RBP2 (Mao S, Neale GAM, Bram RJ, Goorha RM, submitted). phoma and leukemia. [4][5][6] The oncogenic effects of RBTN-2 are Thus, RBTN-2's functions are likely mediated by specific pro-T cell specific. Transgenic mice overexpressing RBTN-2 in all tein-protein interactions through its LIM domains. tissues do not show any type of cancer other than T cellAlthough it is clear that the inappropriate expression of leukemia/lymphoma. 6 The inappropriate expression of RBTN-RBTN-2 in T cells results in leukemia, the molecular 2 in thymocytes causes proliferation of immature T cells prior mechanism(s) of this are not yet understood. Because RBTNto overt leukemia/lymphoma. 5,6 2 exerts its normal functions by binding to different proteins, In addition to its involvement in T cell leukemia/lymphoma, it seemed reasonable to evaluate whether RBTN-2's involve-RBTN-2 plays multiple roles in normal c...

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Fluorescence detection in automated DNA sequence analysis

Cited by 1,526 publications

References 6 publications

First evidence of Lymphocytic Choriomeningitis virus (Arenaviruses) infection in Mus musculus rodents captured in the urban area of the municipality of Sincelejo-Sucre, Colombia

First evidence of Lymphocytic Choriomeningitis virus (Arenaviruses) infection in Mus musculus rodents captured in the urban area of the municipality of Sincelejo-Sucre, Colombia

Low level myocardial parvovirus B19 persistence is a frequent finding in patients with heart disease but unrelated to ongoing myocardial injury

Elf-2, a rhombotin-2 binding ets transcription factor: discovery and potential role in T cell leukemia

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