Fluorescence ELISA based on CAT-regulated fluorescence quenching of CdTe QDs for sensitive detection of FB<sub>1</sub>

Lu, Tianying; Zhan, Shengnan; Zhou, Yaofeng; Chen, Xirui; Huang, Xiaolin; Leng, Yuankui; Xiong, Yonghua; Xu, Youlong

doi:10.1039/c8ay02065e

Cited by 28 publications

(13 citation statements)

References 29 publications

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“…Hitherto, many analytical methods including enzyme-linked immunosorbent assays, , gas chromatography–mass spectrometry, high-performance liquid chromatography, and electrochemical methods , have been established to detect OPs. However, it is regrettable that these methods are somewhat insufficient.…”

Section: Introductionmentioning

confidence: 99%

Capsulation of AuNCs with AIE Effect into Metal–Organic Framework for the Marriage of a Fluorescence and Colorimetric Biosensor to Detect Organophosphorus Pesticides

et al. 2021

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Organophosphorus pesticides (OPs) can inhibit the activity of acetylcholinesterase (AChE) to induce neurological diseases. It is significant to exploit a rapid and sensitive strategy to monitor OPs. Here, a metal–organic framework (MOF) acted as a carrier to encapsulate AuNCs, which can limit the molecular motion of AuNCs, trigger the aggregation-induced emission (AIE) effect, and exhibit a strong fluorescence with a fluorescence lifetime and quantum yield of 6.83 μs and 4.63%, respectively. Then, the marriage of fluorescence and colorimetric signals was realized on the basis of the dual function of the enzymolysis product from AChE and choline oxidase (CHO) on AuNCs@ZIF-8. First, it can decompose ZIF-8 to weaken the restraint on AuNCs, and thus the fluorescence receded. Second, it can be used as a substrate for the peroxidase mimics of the released AuNCs to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) and a visible blue appeared. Thus, on the basis of the inhibition of AChE activity by OPs, a fluorescence–colorimetric dual-signal biosensor was established. In addition, colorimetric paper strips were exploited to realize a visual semiquantitative detection, and a smartphone APP was developed to make the visualization results more precise and realize real-time supervision of pesticide contamination.

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Section: Introductionmentioning

confidence: 99%

Capsulation of AuNCs with AIE Effect into Metal–Organic Framework for the Marriage of a Fluorescence and Colorimetric Biosensor to Detect Organophosphorus Pesticides

et al. 2021

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“…Therefore, researchers have been working to develop various immunoassays for better analytical performance. New procedures such as fluorescent-linked immunosorbent assays (FLISA) and chemiluminescence enzyme immunoassays (CLEIA) can reduce detection duration and improves the sensitivity by relying on enhanced optical signals [36][37][38]. Lateral flow immunoassays (LFIA) and immunosensors have become popular analytical methods in recent years.…”

Section: Immunoassaysmentioning

confidence: 99%

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Yang

Wang

et al. 2021

J Animal Sci Biotechnol

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Mycotoxins are secondary metabolites produced by fungus. Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs. So, powerful detection methods are vital and effective ways to prevent feed contamination. Traditional detection methods can no longer meet the needs of massive, real-time, simple, and fast mycotoxin monitoring. Rapid detection methods based on advanced material and sensor technology are the future trend. In this review, we highlight recent progress of mycotoxin rapid detection strategies in feedstuffs and foods, especially for simultaneous multiplex mycotoxin determination. Immunoassays, biosensors, and the prominent roles of nanomaterials are introduced. The principles of different types of recognition and signal transduction are explained, and the merits and pitfalls of these methods are compared. Furthermore, limitations and challenges of existing rapid sensing strategies and perspectives of future research are discussed.

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“… Representative illustration of nanozyme-based method for the detection of all kinds of toxins in food samples (EC, electrochemistry) ( (A) Nilam et al, 2017 ; (B) Yang et al, 2021 ; (C) Lu et al, 2018 ; (D) Savas and Altintas 2019 ). …”

Section: Application In Food Contaminants Detectionmentioning

confidence: 99%

Nanozyme Applications: A Glimpse of Insight in Food Safety

Zhou

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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Nanozymes own striking merits, including high enzyme-mimicking activity, good stability, and low cost. Due to the powerful and distinguished functions, nanozymes exhibit widespread applications in the field of biosensing and immunoassay, attracting researchers in various fields to design and engineer nanozymes. Recently, nanozymes have been innovatively used to bridge nanotechnology with analytical techniques to achieve the high sensitivity, specificity, and reproducibility. However, the applications of nanozymes in food applications are seldom reviewed. In this review, we summarize several typical nanozymes and provide a comprehensive description of the history, principles, designs, and applications of nanozyme-based analytical techniques in food contaminants detection. Based on engineering and modification of nanozymes, the food contaminants are classified and then discussed in detail via discriminating the roles of nanozymes in various analytical methods, including fluorescence, colorimetric and electrochemical assay, surface-enhanced Raman scattering, magnetic relaxing sensing, and electrochemiluminescence. Further, representative examples of nanozymes-based methods are highlighted for contaminants analysis and inhibition. Finally, the current challenges and prospects of nanozymes are discussed.

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Fluorescence ELISA based on CAT-regulated fluorescence quenching of CdTe QDs for sensitive detection of FB₁

Abstract: A competitive fluorescence enzyme-linked immunosorbent assay (cFELISA) was developed for the highly sensitive detection of fumonisin B1 (FB1) based on the catalase (CAT)-regulated fluorescence quenching of mercaptopropionic acid-modified CdTe quantum dots (MPA-QDs).

Cited by 28 publications

References 29 publications

Capsulation of AuNCs with AIE Effect into Metal–Organic Framework for the Marriage of a Fluorescence and Colorimetric Biosensor to Detect Organophosphorus Pesticides

Capsulation of AuNCs with AIE Effect into Metal–Organic Framework for the Marriage of a Fluorescence and Colorimetric Biosensor to Detect Organophosphorus Pesticides

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Nanozyme Applications: A Glimpse of Insight in Food Safety

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Fluorescence ELISA based on CAT-regulated fluorescence quenching of CdTe QDs for sensitive detection of FB1

Abstract: A competitive fluorescence enzyme-linked immunosorbent assay (cFELISA) was developed for the highly sensitive detection of fumonisin B1 (FB1) based on the catalase (CAT)-regulated fluorescence quenching of mercaptopropionic acid-modified CdTe quantum dots (MPA-QDs).

Cited by 28 publications

References 29 publications

Capsulation of AuNCs with AIE Effect into Metal–Organic Framework for the Marriage of a Fluorescence and Colorimetric Biosensor to Detect Organophosphorus Pesticides

Capsulation of AuNCs with AIE Effect into Metal–Organic Framework for the Marriage of a Fluorescence and Colorimetric Biosensor to Detect Organophosphorus Pesticides

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Nanozyme Applications: A Glimpse of Insight in Food Safety

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Product

Resources

About

Fluorescence ELISA based on CAT-regulated fluorescence quenching of CdTe QDs for sensitive detection of FB₁