1996
DOI: 10.1021/bi9610919
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Fluorescence Emission of Ethidium Bromide Intercalated in Defined DNA Duplexes:  Evaluation of Hydrodynamics Components

Abstract: The arrangement and stacking of noncovalently contiguous double-helical sections are increasingly invoked in single-stranded DNA and RNA tertiary structure. These tertiary structures of nucleic acids are defined by their double stranded regions, and their orientation in the molecular frame constitutes an important component of the nucleic acid structure. A direct view of these tertiary structures can be obtained by fluorescence polarization anisotropy of bound ethidium bromide (EB). The orientation of the dye … Show more

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Cited by 58 publications
(53 citation statements)
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“…In our study, the values of naked ODNs are relatively low as compared with those found by other groups (27)(28)(29). The origin of this discrepancy is not clear but our data show that the steady-state anisotropy is not proportional to the ODN length.…”
Section: Discussioncontrasting
confidence: 92%
“…In our study, the values of naked ODNs are relatively low as compared with those found by other groups (27)(28)(29). The origin of this discrepancy is not clear but our data show that the steady-state anisotropy is not proportional to the ODN length.…”
Section: Discussioncontrasting
confidence: 92%
“…Thus, the 2.1-ns component of the decay is attributed to the EB molecules that are electrostatically bound outside the dsDNA, and the 16.5-ns component is attributed to the emission from the intercalated EB. The 16.5-ns lifetime is comparable with the reported lifetime (22 ns) for EB intercalated in dsDNA (11).…”
Section: Resultssupporting
confidence: 84%
“…The absorption and emission transitions of the conjugated polymer are both parallel to the polymer chain. The emission transition of intercalated EB is nearly perpendicular to the axis of the double helix (dsDNA) with a small distribution of angles about the precisely orthogonal configuration (10,11), a configuration that is unfavorable for FRET.…”
Section: Resultsmentioning
confidence: 99%
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“…As a result of these features ethidium has been widely used as a common fluorescence stain and a lot of experimental data on ethidium have been collected over the last three decades. [21][22][23][24][25][29][30][31][32][33] It has been shown experimentally [12] that the intercalation rate of ethidium to DNA is controlled by the insertion of an aromatic ring into the DNA structure. (Earlier, it was suggested that the process was controlled by the diffusion of ethidium toward the DNA.)…”
Section: Introductionmentioning
confidence: 99%