Fluorescence imaging in the surgical management of liver cancers: Current status and future perspectives

Wang, Qingliang; Li, Xiaojie; Qian, Baifeng; Hu, Kunpeng; Liu, Bo

doi:10.1016/j.asjsur.2021.08.063

Cited by 15 publications

(8 citation statements)

References 68 publications

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“…Takemura et al have pointed that the limitation of ICG fluorescent imaging mainly includes whole liver staining because ICG is absorbed by hepatocytes (30). HCC cells in poorly differentiated or metastatic liver cancer tissues hardly take up ICG, which influence the identification of HCC tissues by ICG (31). Gotoh et al have reported that ICG is not able to distinguish benign and malignant tumors well, and its accumulation in adjacent non-tumor liver tissues leads to a false positive rate of 40-50% during detection of HCC (6,9).…”

Section: Discussionmentioning

confidence: 99%

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Near-infrared fluorescence imaging of hepatocellular carcinoma cells regulated by β-catenin signaling pathway

et al. 2023

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BackgroundNear-infrared fluorescence (NIRF) imaging has recently emerged as a promising tool for noninvasive cancer imaging. However, lack of tumor sensitivity and specificity restricts the application of NIRF dyes in surgical navigation.MethodsHerein, we investigated the imaging features of NIRF dye MHI-148 and indocyanine green (ICG) in live cell imaging and xenograft nude mice models. TCGA dataset analysis and immunohistochemistry were conducted to investigate the expression of OATPs or ABCGs in hepatocellular carcinoma (HCC) tissues. OATPs or ABCGs were knocked down and overexpressed in HCC cells using transient transfection by siRNA and plasmids or stable transfection by lentivirus. Further, qRT-PCR ,Western blotting and the use of agonists or inhibitors targeting β-catenin signaling pathway were applied to explore its important role in regulation of OATP2B1 and ABCG2 expression.ResultsHere we demonstrated that NIRF dye MHI-148 was biocompatible as indocyanine green (ICG) but with higher imaging intensity and preferential uptake and retention in hepatocellular carcinoma (HCC) cells and tissues. Moreover, our data indicated that membrane transporters OATP2B1 and ABCG2, which regulated by β-catenin signaling pathway, mediated tumor-specific accumulation and retention of MHI-148 in HCC cells. In addition, the treatment with β-catenin inhibitor significantly enhanced the accumulation of MHI-148 in HCC tissues and improved the efficacy of tumor imaging with MHI-148 in vivo.ConclusionsOur study uncovers a mechanism that links the distribution and expression of the membrane transporters OATP2B1 and ABCG2 to the tumor-specific accumulation of MHI-148, and provides evidence supporting a regulating role of the β-catenin signaling pathway in OATP2B1 and ABCG2- induced retention of MHI-148 inHCC tissues, and strategy targeting key components of MHI-148 transport machinery may be a potential approach to improve HCC imaging.

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Section: Discussionmentioning

confidence: 99%

“…have pointed that the limitation of ICG fluorescent imaging mainly includes whole liver staining because ICG is absorbed by hepatocytes ( 30 ). HCC cells in poorly differentiated or metastatic liver cancer tissues hardly take up ICG, which influence the identification of HCC tissues by ICG ( 31 ). Gotoh et al.…”

Section: Discussionmentioning

confidence: 99%

Near-infrared fluorescence imaging of hepatocellular carcinoma cells regulated by β-catenin signaling pathway

et al. 2023

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“…Very small amounts of imaging agents (nanometer to femtometer or less) can be detected ( Müller et al, 2013 ). But, the main limitation of FI is that due to the absorption and scattering of light by biological tissues and body fluids, non-specific light absorption limits the depth of penetration to a few millimeters, which makes florescence imaging only suitable for superficial targets or body regions of limited size in clinical ( Wang et al, 2022a ). However, because of low phototoxicity to cells, minimal interference to hemoglobin absorption, low autofluorescence and good tissue penetration of near-infrared (NIR) fluorescence, whose absorption and emission maximums are in the near-infrared region (650-900 nm), near-infrared (NIR) fluorescence imaging is more suitable for tissue and individual imaging.…”

Section: The Application Of Optical Imaging Technologies With Oxidati...mentioning

confidence: 99%

“…However, because of low phototoxicity to cells, minimal interference to hemoglobin absorption, low autofluorescence and good tissue penetration of near-infrared (NIR) fluorescence, whose absorption and emission maximums are in the near-infrared region (650-900 nm), near-infrared (NIR) fluorescence imaging is more suitable for tissue and individual imaging. Therefore, NIR FI has more advantages in surgical imaging ( Weissleder and Ntziachristos, 2003 ; Wang et al, 2022a ). At present, fluorescence imaging is commonly used to detect markers of oxidative stress in optical imaging.…”

Section: The Application Of Optical Imaging Technologies With Oxidati...mentioning

confidence: 99%

The application of optical technology in the diagnosis and therapy of oxidative stress-mediated hepatic ischemia-reperfusion injury

Wang

Shao²,

Chen³

et al. 2023

Front. Bioeng. Biotechnol.

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Hepatic ischemia-reperfusion injury (HIRI) is defined as liver tissue damage and cell death caused by reperfusion during liver transplantation or hepatectomy. Oxidative stress is one of the important mechanisms of HIRI. Studies have shown that the incidence of HIRI is very high, however, the number of patients who can get timely and efficient treatment is small. The reason is not hard to explain that invasive ways of detection and lack of timely of diagnostic methods. Hence, a new detection method is urgently needed in clinic application. Reactive oxygen species (ROS), which are markers of oxidative stress in the liver, could be detected by optical imaging and offer timely and effective non-invasive diagnosis and monitoring. Optical imaging could become the most potential tool of diagnosis of HIRI in the future. In addition, optical technology can also be used in disease treatment. It found that optical therapy has the function of anti-oxidative stress. Consequently, it has possibility to treat HIRI caused by oxidative stress. In this review, we mainly summarized the application and prospect of optical techniques in oxidative stress-induced by HIRI.

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“…In the last decade, by using ICG, the value of intraoperative FMI has been well comprehended in hepatic surgery. The usage of ICG is recommended to be adjusted according to the patient’s liver function [ 17 , 18 ]. At least 2 days prior to surgery is recommended for ICG injection to image the lesion in poorly functional liver [ 19 ].…”

Section: Intraoperative Visualization Of Malignanciesmentioning

confidence: 99%

Intraoperative fluorescence molecular imaging accelerates the coming of precision surgery in China

Zhang

Chi

et al. 2022

Eur J Nucl Med Mol Imaging

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Purpose China has the largest cancer population globally. Surgery is the main choice for most solid cancer patients. Intraoperative fluorescence molecular imaging (FMI) has shown its great potential in assisting surgeons in achieving precise resection. We summarized the typical applications of intraoperative FMI and several new trends to promote the development of precision surgery. Methods The academic database and NIH clinical trial platform were systematically evaluated. We focused on the clinical application of intraoperative FMI in China. Special emphasis was placed on a series of typical studies with new technologies or high-level evidence. The emerging strategy of combining FMI with other modalities was also discussed. Results The clinical applications of clinically approved indocyanine green (ICG), methylene blue (MB), or fluorescein are on the rise in different surgical departments. Intraoperative FMI has achieved precise lesion detection, sentinel lymph node mapping, and lymphangiography for many cancers. Nerve imaging is also exploring to reduce iatrogenic injuries. Through different administration routes, these fluorescent imaging agents provided encouraging results in surgical navigation. Meanwhile, designing new cancer-specific fluorescent tracers is expected to be a promising trend to further improve the surgical outcome. Conclusions Intraoperative FMI is in a rapid development in China. In-depth understanding of cancer-related molecular mechanisms is necessary to achieve precision surgery. Molecular-targeted fluorescent agents and multi-modal imaging techniques might play crucial roles in the era of precision surgery.

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Fluorescence imaging in the surgical management of liver cancers: Current status and future perspectives

Cited by 15 publications

References 68 publications

Near-infrared fluorescence imaging of hepatocellular carcinoma cells regulated by β-catenin signaling pathway

Near-infrared fluorescence imaging of hepatocellular carcinoma cells regulated by β-catenin signaling pathway

The application of optical technology in the diagnosis and therapy of oxidative stress-mediated hepatic ischemia-reperfusion injury

Intraoperative fluorescence molecular imaging accelerates the coming of precision surgery in China

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