2022
DOI: 10.1111/jfs.12982
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Fluorescence immunoassay based on phage mimotope for nontoxic detection of Zearalenone in maize

Abstract: Zearalenone (ZEN) is one of the most common mycotoxin contaminants worldwide. In this study, a phage-based direct competitive fluorescence immunosorbent assay (P-dcFLISA) was developed for the detection of ZEN. In this P-dcFLISA, phage mimotope was used to replace chemically synthesized antigens to improve the safety of experiments. Furthermore, ZnCdSe/ZnS (core/shell) quantum dots (QDs) as fluorescent labeling material replaced chromogenic substrate, which are used in traditional ELISA. The 50% inhibition val… Show more

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Cited by 5 publications
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