Fluorescence in situ hybridization

Fluorescence in situ hybridization-based approaches for detection of 12p overrepresentation, in particular i(12p), in cell lines of human testicular germ cell tumors of adults

“…The presence of an i(12p) is sufficiently specific to classify a cancer of the male gonad as a TGCT [9,10]. In addition, the number of copies of i(12p)s has been suggested to be a prognostic Recently, fluorescence in situ hybridization (FISH), which can be applied on metaphase spreads as well as on interphase nuclei {"interphase cytogenetics"), was intro duced [14][15][16][17]. This technique confirmed the genuine nature of the i(12p) in TGCT [18,19] and showed that all i(12p)-negative TGCT tested so far contained additional 12p sequences [13,20], implying that relative overrepre sentation of 12p sequences is crucial for the development of a clinically manifest TGCT.…”

Accumulation of oocytes and/or embryos by vitrification: a new strategy for managing poor responder patients undergoing pre implantation diagnosis

“…Three different genetic techniques were applied, depending on the indication: fluorescent in situ hybridization (FISH) 7 was used for patients suffering from X-linked microtubular myopathy, Duchene muscular dystrophy, high sperm aneuploidy or recurrent implantation failure; polymerase chain reaction (PCR) 8 was the technique used for patients at risk for offspring with cystic fibrosis. Array complete genome hybridisation techniques (aCGH) 9 were applied for patients at risk for recurrent miscarriage or for reciprocal translocations.…”

Approaches for the detection of harmful algal blooms using oligonucleotide interactions

“…FISH, also referred to as whole cell hybridisation, was first reported in the 1970s; however, it was almost 25 years before this technique was first applied to HAB detection [36][37][38]. A schematic diagram illustrating the FISH process is shown in Fig.…”