Fluorescence in situ hybridization using digital imaging microscopy.

Ballard, S G; Ward, David C.

doi:10.1177/41.12.8245423

Cited by 34 publications

(12 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 16-bit source images were stored as normalized 8-bit gray scale data files by the software program CCD Image Capture (Yale University, New Haven, CT). Highly plane-parallel bandpass filters maintained proper image registration (Ballard and Ward, 1993). Gene Join (Yale University) and an Apple Macintosh computer were used to merge and pseudocolor the images.…”

Section: Metaphase Fish and Image Acquisitionmentioning

confidence: 99%

Coiled Bodies Preferentially Associate with U4, U11, and U12 Small Nuclear RNA Genes in Interphase HeLa Cells but Not with U6 and U7 Genes

Jacobs

Frey

et al. 1999

MBoC

View full text Add to dashboard Cite

Coiled bodies (CBs) are nuclear organelles involved in the metabolism of small nuclear RNAs (snRNAs) and histone messages. Their structural morphology and molecular composition have been conserved from plants to animals. CBs preferentially and specifically associate with genes that encode U1, U2, and U3 snRNAs as well as the cell cycle-regulated histone loci. A common link among these previously identified CBassociated genes is that they are either clustered or tandemly repeated in the human genome. In an effort to identify additional loci that associate with CBs, we have isolated and mapped the chromosomal locations of genomic clones corresponding to bona fide U4, U6, U7, U11, and U12 snRNA loci. Unlike the clustered U1 and U2 genes, each of these loci encode a single gene, with the exception of the U4 clone, which contains two genes. We next examined the association of these snRNA genes with CBs and found that they colocalized less frequently than their multicopy counterparts. To differentiate a lower level of preferential association from random colocalization, we developed a theoretical model of random colocalization, which yielded expected values for 2 tests against the experimental data. Certain single-copy snRNA genes (U4, U11, and U12) but not controls were found to significantly (p Ͻ 0.000001) associate with CBs. Recent evidence indicates that the interactions between CBs and genes are mediated by nascent transcripts. Taken together, these new results suggest that CB association may be substantially augmented by the increased transcriptional capacity of clustered genes. Possible functional roles for the observed interactions of CBs with snRNA genes are discussed.

show abstract

Section: Metaphase Fish and Image Acquisitionmentioning

confidence: 99%

Coiled Bodies Preferentially Associate with U4, U11, and U12 Small Nuclear RNA Genes in Interphase HeLa Cells but Not with U6 and U7 Genes

Jacobs

Frey

et al. 1999

MBoC

View full text Add to dashboard Cite

show abstract

“…The 16-bit source images were stored as normalized 8-bit gray scale data files by use of the software program CCD Image Capture (Yale University, New Haven, CT). Proper image registration was maintained by the use of highly plane parallel bandpass filters (Ballard and Ward 1993). Image merging and pseudocoloring were done with the use of Gene Join (Yale University) on an Apple Macintosh computer.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Characterization of the mouse histone gene cluster on chromosome 13: 45 histone genes in three patches spread over 1Mb.

Wang¹,

Krasikov²,

Frey³

et al. 1996

Genome Res.

View full text Add to dashboard Cite

The histone gene cluster on mouse chromosome 13 has been isolated and characterized. Using overlapping YAC clones containing histone genes from chromosome 13, a contig of -2 Mb has been defined, it contains 45 histone genes, organized in three patches containing tightly clustered genes. An 80-kb patch {patch I!I} containing 12 histone genes is near one end of the contig, and a similar-sized patch {patch i} containing 15 histone genes is near the other end of the contig, located at least 500 kb from the central patch {patch I1} of histone genes. The entire cluster contains six histone HI genes, including the testis-specific histone Hit gene that maps to the middle of the cluster. All nine histone H3 genes in this cluster have been sequenced, and their level of expression determined. Each histone H3 gene is distinct, with five genes encoding the H3.2 protein subtype and four genes encoding the H3.1 protein. They are all expressed, with each histone H3 gene accounting for a small proportion of the total histone H3 mRNA.

show abstract

“…The 16-bit source images were saved as normalized 8-bit gray scale data files by use of CCD image capture (Yale University, New Haven, CT.). Proper registration of the source images was accomplished in two ways: by use of highly plane parallel bandpass filters (Ballard and Ward 1993), and with an excitation filter slider (CRG Electronics}, which selectively excites individual fluorochromes that are viewed with a triple-bandpass emission filter IChromaTech). Equivalent results were obtained with both methods.…”

Section: Chromosomal Localizationmentioning

confidence: 99%

Growth suppression by p18, a p16INK4/MTS1- and p14INK4B/MTS2-related CDK6 inhibitor, correlates with wild-type pRb function.

Guan¹,

Jenkins²,

Li³

et al. 1994

Genes Dev.

666

482

View full text Add to dashboard Cite

The D-type cyclin-dependent kinases CDK4 and CDK6 are complexed with many small cellular proteins (p14, plS, p16, plS, and p20). We have isolated cDNA sequences corresponding to the MTS2 genomic fragment that encodes the CDK4-and CDK6-associated p14 protein. By use of a yeast interaction screen to search for CDK6-interacting proteins, we have also identified an 18-kD human protein, p18, that is a homolog of the cyclin D-CDK4 inhibitors p16 {INK4A/MTS1) and p14 (MTS2/INK4B). Both in vivo and in vitro, p18 interacts strongly with CDK6, weakly with CDK4, and exhibits no detectable interaction with the other known CDKs. Recombinant p18 inhibits the kinase activity of cyclin D-CDK6. Distinct from the p21/p27 family of CDK inhibitors that form ternary complexes with cyclin-CDKs, only binary complexes of p14, p16, and p18 were found in association with CDK4 and/or CDK6. Ectopic expression of p18 or p16 suppresses cell growth with a correlated dependence on endogenous wild-type pRb.[Key Words: Cyclin-dependent kinase inhibitors; cell cycle; CDK4 and CDK6 interacting proteins]

show abstract

Fluorescence in situ hybridization using digital imaging microscopy.

Cited by 34 publications

References 5 publications

Coiled Bodies Preferentially Associate with U4, U11, and U12 Small Nuclear RNA Genes in Interphase HeLa Cells but Not with U6 and U7 Genes

Coiled Bodies Preferentially Associate with U4, U11, and U12 Small Nuclear RNA Genes in Interphase HeLa Cells but Not with U6 and U7 Genes

Characterization of the mouse histone gene cluster on chromosome 13: 45 histone genes in three patches spread over 1Mb.

Growth suppression by p18, a p16INK4/MTS1- and p14INK4B/MTS2-related CDK6 inhibitor, correlates with wild-type pRb function.

Contact Info

Product

Resources

About