Fluorescence in situ hybridization vs. epsilometer test for detection of clarithromycin‐susceptible and clarithromycin‐resistant Helicobacter pylori <i>strains in gastric biopsies from children</i>

Feydt-Schmidt, Anne; Rüssmann, Holger; Lehn, Norbert; Fischer, Alfred; Antoni, Isabel; Störk, D.; Koletzko, Sibylle

doi:10.1046/j.1365-2036.2002.01382.x

Cited by 43 publications

(41 citation statements)

References 37 publications

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“…As a result of these point mutations, clarithromycin cannot get connected to ribosomes and cannot be effective. Because of the technical difficulties in culture and antibiogram implementations of H. pylori and because it is a fastidious microorganism, practical, cheap and applicable methods are required 25 . Molecular diagnosis tests are alternative methods that are easily standardized and are faster than culture based tests 7 .…”

Section: Discussionmentioning

confidence: 99%

Clarithromycin resistance and 23S rRNA gene point mutations of Helicobacter pylori infection in children

et al. 2016

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This study aims to evaluate Helicobacter pylori with clarithromycin resistant genotypes in Manisa region, Turkey. Two hundred patients, who received diagnosis of Helicobacter pylori infection histopathologically, were included. The sex, age and endoscopy indications of the patients were recorded. Polymerase chain reaction method was applied to determine the clarithromycin resistance rate and resistance genotypes at the histologic sections prepared from gastric biopsies that had been embedded in paraffin after fixation by formalin. Helicobacter pylori resistance to clarithromycin was found in 19/200 (9.5%) patients. 10/19 (52.6%) of these clarithromycin-resistant patients had A2143G mutation and 9/19 (47.4%) had A2142G mutation. A2142C mutation on 23S rRNA gene was not detected for any of the patients. Clarithromycin can be used as a first step treatment in the eradication of Helicobacter pylori for the children in our region; if the treatment fails for some patients, clarithromycin resistance, especially A2143G and A2142G mutations should be considered.

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Section: Discussionmentioning

confidence: 99%

Clarithromycin resistance and 23S rRNA gene point mutations of Helicobacter pylori infection in children

et al. 2016

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“…There is no single test with 100% sensitivity and specificity. Culture is the only test with 100% specificity, however, its sensitivity is lower than other tests [7] [8]. Moreover, we should understand the factors that can lead to a false-positive or falsenegative.…”

Section: Discussionmentioning

confidence: 99%

A Retrospective Analysis of Three Non-Invasive Tests for Initial Diagnosis of <i>Helicobacter pylori</i> Infection in Children

Ikuse¹,

Kudo²,

Obayashi³

et al. 2017

AiM

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Proper diagnosis in the pediatric population is required to eradicate Helicobacter pylori (H. pylori) and prevent gastric cancer. Our aim was to assess the performance of non-invasive tests to diagnose H. pylori infection in pediatric patients. A retrospective analysis was performed on 141 pediatric patients requiring endoscopic evaluation and diagnostic tests for H. pylori infection to define the cause of abdominal symptoms. Non-invasive tests included the 13 C-urea breath test (UBT), a monoclonal stool antigen test using enzymelinked immuno-sorbent assay (mSAT), and a serum immunoglobulin G antibody test using antigens derived from Japanese individuals (S-Ab). This study investigated sensitivity, specificity, likelihood ratios for a positive and a negative test (LR + and LR − ), and accuracy of non-invasive tests, in comparison with invasive tests. Eighty two of 141 patients (58%) were recognized as H. pylori positive by invasive methods. When UBT, mSAT or S-Ab were analyzed, all were found to be effective over 94% accurate. Specificity ranged between 86.7% and 95.8%, and sensitivity ranged between 93.8% and 97.1%. When subjects were stratified into three distinct age groups, the best performance was achieved for 1 -6 years old with mSAT at 100% for sensitivity, specificity, and accuracy. S-Ab yielded the best results for children 7 -12 years old and the UBT test performed best for 13 -18 years old. These results demonstrate the utility of UBT, mSAT, and S-Ab non-invasive tests in diagnosing H. pylori but suggest that certain tests may be optimal for children of distinct ages. Three non-invasive tests, UBT, mSAT and S-Ab showed sufficient sensitivity, specificity and accuracy for the initial diagnosis of H. pylori infection among pediatric patients. Non-invasive tests may contribute to achieving minimum invasive diagnosis with combining with a histological test and a culture test in children.

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“…This provides the clinician with important information to make a proper treatment recommendation (29)(30)(31) (29). FISH test is a reliable fast method for the detection of clarithromycin-resistant H. pylori mutants, and results are available within a few hours after an endoscopy .…”

Section: Original Articlementioning

confidence: 99%

“…FISH and RFLP tests can be performed from fresh or frozen materials and the results are obtained very quickly, in as little as 3 hours. The probes used for FISH test are commercially available, and the method is cost-effective and can be applied in any laboratory without the need for special equipment or facilities, except for a fluorescence microscope (29)(30)(31)(32). So we suggest that in suitable institutions clarithromycin resistance testing should be performed using the FISH test.…”

Section: Original Articlementioning

confidence: 99%

Comparison of FISH, RFLP and agar dilution methods for testing clarithromycin resistance of Helicobacter pylori

Çiftçi

Uğraş²,

Acartürk³

et al. 2015

Turk J Gastroenterol

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Background/Aims: Clarithromycin resistance is an important factor of Helicobacter pylori (H. pylori) eradication failure in adults and children. There are some tests to determine resistance such as restriction fragment length polimorphism (RFLP), fluorescence in situ hibridisation (FISH), PCR and (culture) agar dilution. Clarithromycin resistance is reported between 16.8%-48.2% in Turkey using PCR, 18% in Japan using RFLP. The aim of the study is to compare the efficacy of FISH, RFLP and culture. Materials and Methods: Patients with gastric complaint underwent endoscopic examination. H. pylori status of all patients was tested with urea breath test. Gastric biopsy samples obtained from adult patients and children were studied. Each tissue was analised with FISH, PCR-RFLP anda gar dilution. Results: A total 100 patients were positive by UBT and histology for H. pylori. Tissues from 89 adults and 11 children were evaluated. According to FISH and RFLP clarithromycin resistance was 26% and 16% respectively. Among 100 patients H. pylori was cultured in 52 tissue samples, among these samples 7 were resistant to clarithromycin. There was strong correlation between the results of FISH and RFLP; RFLP and culture; and FISH and culture. Conclusion: There is a high ratio of clarithromycin resistance in the studied population. All 3 tests are valuable, but FISH seems to be more sensitive among these tests. We suggest FISH should be used for detecting clarithromycin resistance among H. pylori infected patients before eradication therapy.

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Fluorescence in situ hybridization vs. epsilometer test for detection of clarithromycin‐susceptible and clarithromycin‐resistant Helicobacter pylori strains in gastric biopsies from children

Cited by 43 publications

References 37 publications

Clarithromycin resistance and 23S rRNA gene point mutations of Helicobacter pylori infection in children

Clarithromycin resistance and 23S rRNA gene point mutations of Helicobacter pylori infection in children

A Retrospective Analysis of Three Non-Invasive Tests for Initial Diagnosis of <i>Helicobacter pylori</i> Infection in Children

Comparison of FISH, RFLP and agar dilution methods for testing clarithromycin resistance of Helicobacter pylori

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Fluorescence in situ hybridization vs. epsilometer test for detection of clarithromycin‐susceptible and clarithromycin‐resistant Helicobacter pylori strains in gastric biopsies from children

Cited by 43 publications

References 37 publications

Clarithromycin resistance and 23S rRNA gene point mutations of Helicobacter pylori infection in children

Clarithromycin resistance and 23S rRNA gene point mutations of Helicobacter pylori infection in children

A Retrospective Analysis of Three Non-Invasive Tests for Initial Diagnosis of &lt;i&gt;Helicobacter pylori&lt;/i&gt; Infection in Children

Comparison of FISH, RFLP and agar dilution methods for testing clarithromycin resistance of Helicobacter pylori

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A Retrospective Analysis of Three Non-Invasive Tests for Initial Diagnosis of <i>Helicobacter pylori</i> Infection in Children