Fluorescence line narrowing: a high-resolution window on DNA and protein damage from chemical carcinogens

Jankowiak, Ryszard; Small, Gerald J.

doi:10.1021/tx00021a001

Cited by 92 publications

(118 citation statements)

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“…In FLNS a tunable laser is used to select a subset (isochromat) of molecules with identical S 1 ← S 0 transition energies. When the experiment is carried out at cryogenic temperatures this selection is maintained during the lifetime of the excited state and this subset will show line-narrowed fluorescence (see [29,30] for details on the method and applications). FLNS has been applied as a stand-alone technique, for the off-line identification of HPLC fractions, or for the off-line identification of TLC-separated spots [31,32].…”

Section: Coupling Of Ce To Fluorescence Line-narrowingmentioning

confidence: 99%

Capillary electrophoresis with laser-induced fluorescence detection for natively fluorescent analytes

Gooijer¹,

Kok²,

Ariese³

2000

Analusis

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Section: Coupling Of Ce To Fluorescence Line-narrowingmentioning

confidence: 99%

Capillary electrophoresis with laser-induced fluorescence detection for natively fluorescent analytes

Gooijer¹,

Kok²,

Ariese³

2000

Analusis

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“…Fluorescence line-narrowing spectroscopy (FLNS) is a low-temperature spectroscopic technique which has been successfully employed over the years in detection/ characterization of a wide variety of systems, e.g., organic molecules, [1][2][3] inorganic systems, 4 DNA and protein adducts (Ref. 5 and references therein).…”

Section: Introductionmentioning

confidence: 99%

Modeling of fluorescence line-narrowed spectra in weakly coupled dimers in the presence of excitation energy transfer

Lin

Reppert

Feng

et al. 2014

The Journal of Chemical Physics

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This work describes simple analytical formulas to describe the fluorescence line-narrowed (FLN) spectra of weakly coupled chromophores in the presence of excitation energy transfer (EET). Modeling studies for dimer systems (assuming low fluence and weak coupling) show that the FLN spectra (including absorption and emission spectra) calculated for various dimers using our model are in good agreement with spectra calculated by: (i) the simple convolution method and (ii) the more rigorous treatment using the Redfield approach [T. Renger and R. A. Marcus, J. Chem. Phys. 116, 9997 (2002)]. The calculated FLN spectra in the presence of EET of all three approaches are very similar. We argue that our approach provides a simplified and computationally more efficient description of FLN spectra in the presence of EET. This method also has been applied to FLN spectra obtained for the CP47 antenna complex of Photosystem II reported by Neupane et al. [J. Am. Chem. Soc. 132, 4214 (2010)], which indicated the presence of uncorrelated EET between pigments contributing to the two lowest energy (overlapping) exciton states, each mostly localized on a single chromophore. Calculated and experimental FLN spectra for CP47 complex show very good qualitative agreement.

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“…Thus, their optical transition overlap each other at fixed position as shown in Figure 6A Fluorescence line narrowing technique was first applied to gases by Feld and Javan in 1969 [118] and subsequently to ionic solids by Szabo [119] and to molecular solids by Personov et al [120,121]. Since then it has been studied from a variety points of view [122][123][124][125][126][127]. Figure 7 is a schematic representation of an inhomogeneously broadened electronic absorption origin band, (0,0), at low temperature.…”

Section: B Principles Of Fluorescence Line Narrowingmentioning

confidence: 99%

“…As discussed before, however, the PSB profiles depend on the phonon density of state and electron-phonon coupling constant. For PAH in glass, the 1-phonon density profile has a maximum at 0)^^ ~ 25 cm"^ and a width of F ~ 40 cm"\ The r-phonon profile has a maximum at rco and a width of ~r'^r [127,129]. From the Debye-Waller factor given by Eq.…”

Section: B Principles Of Fluorescence Line Narrowingmentioning

confidence: 99%

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High resolution fluorescence studies of metabolic pathways of DNA and oligonucleotide adducts from polycyclic aromatic compounds

Jeong

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UMI MICROFILMED 1991 INFORMATION TO USERSThis manuscript has been reproduced from the microfflm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer.The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction.In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand corner and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book.Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6" x 9" black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. 1991Signature was redacted for privacy.Signature was redacted for privacy.Signature was redacted for privacy.ii Ultraviolet and X-ray light have long beeHxknown to be highly cancer-causing and mutagenic agents [16]. For example, exposure of skin to UV light can induce skin cancer, while X-ray exposure to the thyroid induces thyroid cancer. Electromagnetic radiation, however, may not be assumed to cause all the cells in an exposed population to become cancerous since radiation can also produce many other deleterious changes leading to cell death. A radiation dose sufficient to induce cancer in all cells will virtually kill every cell. Not only can radiation induce simple mutation, it can also produce chromosome fragmentation, resulting in large deletions of genetic material. Thus radiation causes a variety of genetic changes, many of which might contribute to the cancerous state. A well known deleterious effect, xeroderma pigmentosum, is magnified in certain individuals. These people are homozygous for a recessive mutation that inactivates a gene involved in repairing thymine dimers, the major damage caused by UV Similar studies have shown that relatively weak carcinogens are also metabolized by monooxgenation mechanism. In most cases, however, the evidence is much less complete than in the case of B[a]P and rests primarily on comparison of the relative mutagenicities and tumorigenicities of the isomeric dihydrodiols or the corresponding diol epoxides obtained through synthesis. One-electron oxidation and metabolitesThe monooxygenation mechanism, which emphasizes the important role of the bay-region diol epoxide, has been widely accepted during last 15 years...

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Fluorescence line narrowing: a high-resolution window on DNA and protein damage from chemical carcinogens

Cited by 92 publications

References 61 publications

Capillary electrophoresis with laser-induced fluorescence detection for natively fluorescent analytes

Capillary electrophoresis with laser-induced fluorescence detection for natively fluorescent analytes

Modeling of fluorescence line-narrowed spectra in weakly coupled dimers in the presence of excitation energy transfer

High resolution fluorescence studies of metabolic pathways of DNA and oligonucleotide adducts from polycyclic aromatic compounds

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