Fluorescence parameters as early indicators of light stress in barley

Kalaji, Hazem M.; Carpentier, Robert; Allakhverdiev, Suleyman I.; Bosa, Karolina

doi:10.1016/j.jphotobiol.2012.03.009

Cited by 215 publications

(142 citation statements)

References 45 publications

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“…For example, the extent of the I-P loss in barley varieties depends on their drought tolerance (Oukarroum et al 2009;Ceppi et al 2012). ChlF is emitted following a dark-to-light transition of a photosynthetic sample, while delayed fluorescence emission (DF) occurs during light-to-dark transitions (Goltsev et al 2009;Strasser et al 2010;Kalaji et al 2012). DF is thought to reflect the recombination (in the dark) between the reduced primary electron acceptor Q A -and the oxidized donor (P680 ? )…”

Section: Drought Stressmentioning

confidence: 99%

“…These can disrupt the photosynthetic apparatus, causing a decrease of plant productivity and overall yield. Photosynthesis is particularly sensitive to environmental constraints (see Kalaji et al 2012), making photosynthetic measurements an important component of plant stress studies. However, traditional methods, even technically advanced ones such as the measurements of photosynthetic rates through gas exchange (CO 2 , H 2 O, and O 2 ), are time-consuming and provide incomplete information on overall photosynthetic function.…”

Section: Introductionmentioning

confidence: 99%

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Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions

et al. 2016

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Plants living under natural conditions are exposed to many adverse factors that interfere with the photosynthetic process, leading to declines in growth, development, and yield. The recent development of Chlorophyll a fluorescence (ChlF) represents a potentially valuable new approach to study the photochemical efficiency of leaves. Specifically, the analysis of fluorescence signals provides detailed information on the status and function of Photosystem II (PSII) reaction centers, lightharvesting antenna complexes, and both the donor and acceptor sides of PSII. Here, we review the results of fast ChlF analyses of photosynthetic responses to environmental stresses, and discuss the potential scientific and practical applications of this innovative methodology. The recent availability of portable devices has significantly expanded the potential utilization of ChlF techniques, especially for the purposes of crop phenotyping and monitoring.

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Section: Drought Stressmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions

et al. 2016

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“…In addition to conventional saturation pulse method of chlorophyll fluorescence (Schreiber, 1986) measured simultaneously with gas exchange or P700 absorbance, we applied also the analysis of fast chlorophyll a fluorescence induction. This analytical tool can serve as a valid examination of environmental effects on the photosynthetic apparatus ( Ziv cák et al, 2008;Kalaji et al, 2012;Bresti c et al, 2012). Our results indicate that the mutation of the enzyme not directly associated with conversion of light energy led to surprisingly significant modifications of structure and function of photosystems.…”

Section: Introductionmentioning

confidence: 68%

Reduced glutamine synthetase activity plays a role in control of photosynthetic responses to high light in barley leaves

Brestič

Živčák

Olšovská

et al. 2014

Plant Physiology and Biochemistry

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a b s t r a c tThe chloroplastic glutamine synthetase (GS, EC 6.3.1.2) activity was previously shown to be the limiting step of photorespiratory pathway. In our experiment, we examined the photosynthetic high-light responses of the GS2-mutant of barley (Hordeum vulgare L.) with reduced GS activity, in comparison to wild type (WT). The biophysical methods based on slow and fast chlorophyll fluorescence induction, P700 absorbance, and gas exchange measurements were employed. Despite the GS2 plants had high basal fluorescence (F 0 ) and low maximum quantum yield (F v /F m ), the CO 2 assimilation rate, the PSII and PSI actual quantum yields were normal. On the other hand, in high light conditions the GS2 had much higher non-photochemical quenching (NPQ), caused both by enhanced capacity of energy-dependent quenching and disconnection of PSII antennae from reaction centers (RC). GS2 leaves also maintained the PSII redox poise (Q A À /Q A total) at very low level; probably this was reason why the observed photoinhibitory damage was not significantly above WT. The analysis of fast chlorophyll fluorescence induction uncovered in GS2 leaves substantially lower RC to antenna ratio (RC/ABS), low PSII/PSI ratio (confirmed by P700 records) as well as low PSII excitonic connectivity.

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“…According to Kalaji et al (2016), the recent development of chlorophyll a fluorescence measurement methods represents a valuable new approach that can be used to study the photochemical efficiency of leaves and provide details on the status and function of the PSII reaction centres, the donor and acceptor sides of PSII and light-harvesting antenna complexes. Because photosynthesis is sensitive to environmental factors, fluorescence measurements are important components of plant stress studies (Kalaji et al 2012;Murchie and Lawson 2013).…”

Section: Discussionmentioning

confidence: 99%

Rooting affects the photosystem II activity: in vitro and ex vitro studies on energy hybrid sorrel

et al. 2017

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Rumex tianschanicus 9 Rumex patientia is a high-biomass-yielding plant suitable for fuel and biogas production. The protocol of the hybrid sorrel micropropagation was used to study the changes in the photosystem II (PSII) activity as well as to analyse the ultrastructure of the chloroplasts. The lowest effective PSII quantum yield [Y(II)] and an apparent electron transport rate of PSII [ETR(II)] were observed for adventitious shoots that had been regenerated in vitro, before rooting. These fluorescence parameters were higher and similar for both the leaves of the same adventitious shoots that had been rooted under in vitro conditions and for the shoots that had been acclimated and grown in ex vitro conditions. The analysis indicated that the PSII activity strongly depends on the formation of properly functioning roots and that in vitro or ex vitro culture conditions are, at least to some degree, less important. TEM analysis revealed that chloroplasts from plants rooted in vitro were sufficiently mature and acclimatization processes have less impact on their development. This is the first report concerning the analysis of PSII activity and the ultrastructure of the chloroplasts at all of the stages of micropropagation, i.e. adventitious shoot formation in vitro, rooting in vitro and acclimation to ex vitro conditions. It strongly indicated that rooting under in vitro conditions, rather than the acclimation to ex vitro conditions, plays a key role in the development of a completely functional photosynthetic apparatus in hybrid sorrel.Keywords Rumex tianschanicus 9 Rumex patientia Á PSII activity Á Chlorophyll a fluorescence Á Ultrastructure of chloroplasts Á In vitro/ex vitro transfer Á Photosynthetic apparatus

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Fluorescence parameters as early indicators of light stress in barley

Cited by 215 publications

References 45 publications

Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions

Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions

Reduced glutamine synthetase activity plays a role in control of photosynthetic responses to high light in barley leaves

Rooting affects the photosystem II activity: in vitro and ex vitro studies on energy hybrid sorrel

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