Fluorescence Polarization Immunoassay Based on a New Monoclonal Antibody for the Detection of the Zearalenone Class of Mycotoxins in Maize

Zhang, Xiya; Eremin, Sergei A.; Wen, Kai; Yu, Xuezhi; Li, Chenglong; Ke, Yuebin; Jiang, Haiyang; Shen, Jianzhong; Wang, Zhanhui

doi:10.1021/acs.jafc.6b05614

Cited by 88 publications

(43 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The labeled analyte was not necessary to be separated after the antibody-analyte binging reaction. FPIA was applied to determine pesticides (Stevanovic et al, 2017) and mycotoxins (Zhang et al, 2017). It meets the requirement of simple, cost-effective, fast and high sample throughput.…”

Section: Introductionmentioning

confidence: 99%

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Liu

Wang

Zhou

et al. 2017

Food and Agricultural Immunology

Self Cite

View full text Add to dashboard Cite

The herbicide propisochlor can be introduced into the water cycle via agricultural activities, thus possibly leading to harmful ecological effects. It is significant for the development of a rapid method for propisochlor analysis in water. In this work, a rapid homogeneous immunoassay based on fluorescence polarization immunoassay (FPIA) was firstly developed for the determination of propisochlor in pond water. The developed FPIA assay can be completed within only 14 min; the 50% inhibition concentration (IC 50 ) was 125.1 ng/mL and the limit of detection was 8.4 ng/mL. The recoveries of propisochlor in pond water ranged from 80.0% to 86.6%. A high performance liquid chromatography confirmation showed a good correlation with the developed FPIA (R 2 = 0.9748). The developed FPIA exhibited satisfactory rapidity, high sensitivity and excellent specificity, meeting the standards of the screening assay of propisochlor in pond water on site. The molecular modeling showed that the alkoxyalkyl of chloroacetamide herbicides exerted important effects on the herbicide-antibody binding.

show abstract

Section: Introductionmentioning

confidence: 99%

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Liu

Wang

Zhou

et al. 2017

Food and Agricultural Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, the system has the potential to be miniaturized and used for on-site, field application. There are several reports on the development of novel immunoassays for detection of ZEN in a static system [26][27][28][29] and in a flow system [30][31], however this work is the first report on ZEN detection using a fully automated immunoassay system.…”

Section: Introductionmentioning

confidence: 98%

Development of an automated flow‐based spectrophotometric immunoassay for continuous detection of zearalenone

Jantra

Zór

Sanders

et al. 2020

Biotechnology and Applied Biochemistry

View full text Add to dashboard Cite

show abstract

“…In recent years, different immunoassay methods based on ZEN monoclonal antibodies (mAbs) with a high affinity and broad class specificity have been established to rapidly detect TZEN. These include an enzyme-linked immunosorbent assay (ELISA) [22][23][24], a gold immunochromatographic assay (GICA) [25], and a fluorescence polarization immunoassay (FPIA) [26]. However, these immunoassay methods have some drawbacks, such as poor specificity and sensitivity to TZEN, possibly due to a low quality mAb, thus not meeting the actual detection needs.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Monoclonal Antibodies with High Affinity and Broad Class Specificity against Zearalenone and Its Major Metabolites

Wang

Zhang

et al. 2021

Toxins

View full text Add to dashboard Cite

This study aimed to detect and monitor total Zearalenone (ZEN) and its five homologs (ZENs) in cereals and feed. The monoclonal antibodies (mAbs) with a high affinity and broad class specificity against ZENs were prepared, and the conditions of a heterologous indirect competitive ELISA (icELISA) were preliminarily optimized based on the ZEN mAbs. The immunogen ZEN-BSA was synthesized using the oxime active ester method (OAE) and identified using infrared (IR) and ultraviolet (UV). The coating antigen ZEN-OVA was obtained via the 1,4-butanediol diglycidyl ether method (BDE). Balb/c mice were immunized using a high ZEN-BSA dose with long intervals and at multiple sites. A heterologous indirect non-competitive ELISA (inELISA) and an icELISA were used to screen the suitable cell fusion mice and positive hybridoma cell lines. The ZEN mAbs were prepared by inducing ascites in vivo. The standard curve was established, and the sensitivity and specificity of the ZEN mAbs were determined under the optimized icELISA conditions. ZEN-BSA was successfully synthesized at a conjugation ratio of 17.2:1 (ZEN: BSA). Three hybridoma cell lines, 2D7, 3C2, and 4A10, were filtered, and their mAbs corresponded to an IgG1 isotype with a κ light chain. The mAbs titers were between (2.56 to 5.12) × 102 in supernatants and (1.28 to 5.12) × 105 in the ascites. Besides, the 50% inhibitive concentration (IC50) values were from 18.65 to 31.92 μg/L in the supernatants and 18.12 to 31.46 μg/L in the ascites. The affinity constant (Ka) of all of the mAbs was between 4.15 × 109 and 6.54 × 109 L/mol. The IC50 values of mAb 2D7 for ZEN, α-ZEL, β-ZEL, α-ZAL, β-ZAL and ZAN were 17.23, 16.71, 18.27, 16.39, 20.36 and 15.01 μg/L, and their cross-reactivities (CRs, %) were 100%, 103.11%, 94.31%, 105.13%, 84.63%, and 114.79%, respectively, under the optimized icELISA conditions. The limit of detection (LOD) for ZEN was 0.64 μg/L, and its linear working range was between 1.03 and 288.55 μg/L. The mAbs preparation and the optimization of icELISA conditions promote the potential development of a rapid test ELISA kit, providing an alternative method for detecting ZEN and its homologs in cereals and feed.

show abstract

Fluorescence Polarization Immunoassay Based on a New Monoclonal Antibody for the Detection of the Zearalenone Class of Mycotoxins in Maize

Cited by 88 publications

References 40 publications

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Rapid and homologous immunoassay for the detection of herbicide propisochlor in water

Development of an automated flow‐based spectrophotometric immunoassay for continuous detection of zearalenone

Preparation and Characterization of Monoclonal Antibodies with High Affinity and Broad Class Specificity against Zearalenone and Its Major Metabolites

Contact Info

Product

Resources

About