Fluorescence Polarization Immunoassay Based on a Monoclonal Antibody for the Detection of the Organophosphorus Pesticide Parathion-methyl

Kolosova, A. S.; Park, Jung Hyun; Еремин, С. А.; Kang, Sang‐Woo; Chung, Duck-Hwa

doi:10.1021/jf025801v

Cited by 72 publications

(41 citation statements)

References 38 publications

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“…A number of biosensors have been developed based on fluorescence polarization immunoassays (FPIA) [45–48]. One example reported by Kolosova et al .…”

Section: Advances In Detection Of Op Compoundsmentioning

confidence: 99%

Fluorescent Chemosensors for Toxic Organophosphorus Pesticides: A Review

Obare

Guo

et al. 2010

Sensors

131

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Many organophosphorus (OP) based compounds are highly toxic and powerful inhibitors of cholinesterases that generate serious environmental and human health concerns. Organothiophosphates with a thiophosphoryl (P=S) functional group constitute a broad class of these widely used pesticides. They are related to the more reactive phosphoryl (P=O) organophosphates, which include very lethal nerve agents and chemical warfare agents, such as, VX, Soman and Sarin. Unfortunately, widespread and frequent commercial use of OP-based compounds in agricultural lands has resulted in their presence as residues in crops, livestock, and poultry products and also led to their migration into aquifers. Thus, the design of new sensors with improved analyte selectivity and sensitivity is of paramount importance in this area. Herein, we review recent advances in the development of fluorescent chemosensors for toxic OP pesticides and related compounds. We also discuss challenges and progress towards the design of future chemosensors with dual modes for signal transduction.

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“…A number of biosensors have been developed based on fluorescence polarization immunoassays (FPIA) [45–48]. One example reported by Kolosova et al .…”

Section: Advances In Detection Of Op Compoundsmentioning

confidence: 99%

Fluorescent Chemosensors for Toxic Organophosphorus Pesticides: A Review

Obare

Guo

et al. 2010

Sensors

131

View full text Add to dashboard Cite

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“…This biosensor was found to be very sensitive (capable of detecting nanomolar (nM) concentrations of paraoxon when exposed to the solution containing the analyte for ten minutes), and it demonstrated some selectivity toward different OP compounds (Rogers et al 1991). A number of biosensors have been developed based on fluorescence polarization immunoassays (FPIA) (Kolosova et al 2003;Kolosova et al 2004;Lee et al 2005;Tang et al 2008). A rapid, fiber-optic biosensor assay for the direct detection of organophosphates was developed to provide continual remote monitoring and spectral fluorescent notification.…”

Section: Fluorescence Based Detection Of Pesticides and Organo-phosphmentioning

confidence: 99%

Organophosphorous Compounds-Toxicity and Detection Approach

Upadhyay¹,

Sharma²,

Rao³

et al. 2011

Pesticides - Strategies for Pesticides Analysis

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“…The ease of analysis, no need for water sample preparation, the stability of immunoreagents, adequate accuracy and reproducibility of results are among the advantages of FPIA. Procedures for the FPIA determination of pesticides have long been successfully developed in our laboratory [30,31] and in cooperation with it [32][33][34][35][36][37][38]. Thus, FPIA procedures for the determination of the following pesticides were described: 2,4-D; 2,4,5-T; pentachlorophenol; atrazine; propanil; DDT; chlorsulfuron; metsulfuron-methyl; parathion-methyl; etc.…”

Section: Acetochlormentioning

confidence: 99%

Determination of the herbicide acetochlor by fluorescence polarization immunoassay

et al. 2005

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Acetochlor[2-chloro-N -(ethoxymethyl)-N -(2-ethyl-6-methylphenyl)acetamide] belongs to chloroacetanilide herbicides, which have been widely introduced into the world agricultural practice [1][2][3]. Chloroacetanilide herbicides are relatively unstable in the environment and undergo degradation within six months; this is a valuable property of these herbicides. However, they can be detected in reservoirs, fish, and products of plant origin in amounts higher than maximum permissible levels as a result of their agricultural use. Acetochlor is a comparatively new pesticide; it was first registered in the United States in 1994. It has now replaced related compounds because of its better biodegradability and a relatively small carcinogenic effect. However, as well as the majority of pesticides, acetochlor is genotoxic, as was exemplified in plants, rats, and human lymphocytes [1]. Therefore, acetochlor residues should be monitored in natural samples such as water and soil.Chromatographic techniques, such as gas chromatography with atomic emission [4] or mass-spectrometric detection [5, 6], high-performance liquid chromatography with various detection techniques [7,8], and capillary electrophoresis [9] are commonly used for determining herbicides, in particular, acetochlor. These techniques are highly sensitive and accurate; however, they are also expensive and time-consuming. At the same time, it is necessary to perform simple and rapid screening in a great number of samples in order to detect herbicide-contaminated areas.Immunochemical techniques based on the specific binding of an analyte to corresponding specific antibodies are well suitable for this purpose. Enzyme immunoassay (EIA) has received the widest acceptance; the theory and practice of EIA was described in detail [10]. The use of EIA for the determination of pesticides has been reported in many reviews [11][12][13][14][15][16].EIA techniques are highly sensitive and simple; they can be performed under field conditions at the sampling site [17]. Since the 1990s, EIA has been used with increasing frequency for the screening of pesticides in natural and food samples. The current status, applicability, and prospects for the widespread use of immunochemical techniques for the determination of pesticides in the environment were considered in a review [18]. Recent data on the development and use of EIA for pesticides in Russia were published in [19][20][21].Earlier, we developed immunochemical methods for the determination of acetochlor by ELISA [22] and with the use of an immunosensor [23] or a piezosensor [24]. However, these are multistep procedures, and the time of analysis is 2 to 3 h. Moreover, ELISA includes a number of steps of pipetting and washing with the use of several specific reagents; it requires qualified personnel. Therefore, the recent trend has been toward the development of nonseparation (homogeneous) immunoassay techniques, which significantly improve socalled high-throughput screening (HTS) [25]. Although an optimum immunoassay procedure for...

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Fluorescence Polarization Immunoassay Based on a Monoclonal Antibody for the Detection of the Organophosphorus Pesticide Parathion-methyl

Cited by 72 publications

References 38 publications

Fluorescent Chemosensors for Toxic Organophosphorus Pesticides: A Review

Fluorescent Chemosensors for Toxic Organophosphorus Pesticides: A Review

Organophosphorous Compounds-Toxicity and Detection Approach

Determination of the herbicide acetochlor by fluorescence polarization immunoassay

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