Fluorescence properties of plant anthocyanin pigments. I. Fluorescence of anthocyanins in Brassica oleracea L. extracts

Drabent, Regina; Pliszka, Barbara; Olszewska, Teresa

doi:10.1016/s1011-1344(99)00070-6

Cited by 54 publications

(37 citation statements)

References 12 publications

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“…Anthocyanin extracts from red cabbage (Brassica oleracea) were previously shown to fluoresce with peaks at 363, 434, and 519 nm (Drabent et al, 1999). Our studies, however, identified significant fluorescence in vivo for total anthocyanins and for individual pigments above 565 nm (Fig.…”

Section: Discussionmentioning

confidence: 50%

“…The difference in our results with those previously reported is likely a consequence of the red cabbage extracts containing complex mixtures of anthocyanins with other phenolics and proteins. Indeed, when the red cabbage extracts were subjected to chromatographic separation, one of the peaks (peak 10; Drabent et al, 1999) displayed significant fluorescence increase in the 550-to 650-nm range (Drabent et al, 1999). Our results, demonstrating that anthocyanins can have fluorescence properties compatible with GFP visualization, pave the way for similar colocalization studies to be carried out in other plants.…”

Section: Discussionmentioning

confidence: 54%

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A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions

Poustka¹,

Irani²,

Feller³

et al. 2007

Plant Physiology

205

230

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Plants produce a very large number of specialized compounds that must be transported from their site of synthesis to the sites of storage or disposal. Anthocyanin accumulation has provided a powerful system to elucidate the molecular and cellular mechanisms associated with the intracellular trafficking of phytochemicals. Benefiting from the unique fluorescent properties of anthocyanins, we show here that in Arabidopsis (Arabidopsis thaliana), one route for anthocyanin transport to the vacuole involves vesicle-like structures shared with components of the secretory pathway. By colocalizing the red fluorescence of the anthocyanins with green fluorescent protein markers of the endomembrane system in Arabidopsis seedlings, we show that anthocyanins are also sequestered to the endoplasmic reticulum and to endoplasmic reticulum-derived vesicle-like structures targeted directly to the protein storage vacuole in a Golgi-independent manner. Moreover, our results indicate that vacuolar accumulation of anthocyanins does not depend solely on glutathione S-transferase activity or ATP-dependent transport mechanisms. Indeed, we observed a dramatic increase of anthocyanin-filled subvacuolar structures, without a significant effect on total anthocyanin levels, when we inhibited glutathione S-transferase activity, or the ATP-dependent transporters with vanadate, a general ATPase inhibitor. Taken together, these results provide evidence for an alternative novel mechanism of vesicular transport and vacuolar sequestration of anthocyanins in Arabidopsis.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 54%

A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions

Poustka¹,

Irani²,

Feller³

et al. 2007

Plant Physiology

205

230

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“…UV-Visible spectrophotometry is one of the most widespread of anthocyanin spectroscopic methods. The method was also applied for the structural change of anthocyanin under the influence of different physicochemical factors and the process of polymerization of anthocyanins 80 . Since their color and stability depend on several factors, much attention has been focused on the development of sensitive and reliable analytical methods for nondestructive quantification of anthocyanins in fruit juices 50 .…”

Section: Anthocyaninsmentioning

confidence: 99%

“…In general, anthocyanins are weakly fluorescent in solution, probably due to quenching of the efficient excited state proton transfer to water. Lack of information of anthocyanins luminescence related to their pigments has been limited in the literature 80 . Fluorescence properties of the chalkone isomer of malvidin 3,5-diglucoside in aqueous solution was studied 81 .…”

Section: Anthocyaninsmentioning

confidence: 99%

“…It was found that long-wavelength fluorescence (centered at 495 nm) observed in the fluorescence spectra of cis-chalcone is ascribed to emitting species formed during the excited state of the chalcone form 81,82 . The fluorescence of cyanidin and malvidin glycosides in aqueous environment was investigated and found that Cya-3-glc exhibits short-wavelength fluorescence at l max 299 nm which was most effectively excited at 220 nm 80 . Similar short-wavelength fluorescence was observed for Cya-3,5-diglc (l max 308 nm) and Mv-3,5-diglc (l max 293 nm) in a binary solvent system.…”

Section: Anthocyaninsmentioning

confidence: 99%

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Phenolics and Ascorbic Acid Related to Antioxidant Activity of MaoFruit Juice and Their Thermal Stability Study (Review Article)

Sripakdee¹,

Mahachai²,

Chanthai³

2017

Orient. J. Chem

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Antioxidant and/or anti-aging activities are always lined with people's minds as major potential benefits concerning human health in the recent commercial features for an economic world of foodstuffs and medical uses. Total phenolics including flavonoids and anthocyanins, and ascorbic acid in the Mao juices are closely related to their antioxidant activity. Numerous research approaches on these functional foods, in particular the colored fruits and vegetables have been investigated. Method validation and determination of the potential compounds have been increasingly developed with highly sensitive and selective procedures and applications including thermal stability of the Mao juice. Their antioxidant activities obtained from different assays related to the contents of both phenolics and ascorbic acid in the anthocyanins-rich Mao juices in Thailand are reported and discussed.

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Cryoconcentration of flavonoid extract for enhanced biophotovoltaics and pH sensitive thin films

Demırbas

Groszman

Pazmiño-Hernandez

et al. 2017

Biotechnology Progress

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Flavonoids are important value added products for dye sensitized solar cells biosensors, functional foods, medicinal supplements, nanomaterial synthesis, and other applications. Brassica oleracea contains high levels of anthocyanins in leaf sap vacuoles, and there are many viable extraction techniques that vary in terms of simplicity, environmental impact, cost, and extract photochemical/electrochemical properties. The efficiency of value added biotechnologies from flavonoid is a function of anthocyanin activity/concentration and molecule stability (i.e., ability to retain molecular resonance under a wide range of conditions). In this paper, we show that block cryoconcentration and partial thawing of anthocyanin from B. oleracea is a green, facile, and highly efficient technique that does not require any special equipment or protocols for producing enhanced value added products. Cryoconcentration increased anthocyanin activity and total phenol content approximately 10 times compared with common extraction techniques. Cryoconcentrated extract had enhanced electrochemical properties (higher oxidation potential), improved chroma, and higher UV absorbance than extract produced with other methods for a pH range of 2-12, with minimal effect on the diffusion coefficient of the extract. As a proof of concept for energy harvesting and sensor applications, dye sensitized solar cells and pH-sensitive thin films were prepared and tested. These devices were comparable with other recently published biotechnologies in terms of efficacy, but did not require expensive/environmentally detrimental extraction or concentration methods. This low cost, biorenewable, and simple method can be used for development of a variety of value added products. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:206-217, 2018.

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Fluorescence properties of plant anthocyanin pigments. I. Fluorescence of anthocyanins in Brassica oleracea L. extracts

Cited by 54 publications

References 12 publications

A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions

A Trafficking Pathway for Anthocyanins Overlaps with the Endoplasmic Reticulum-to-Vacuole Protein-Sorting Route in Arabidopsis and Contributes to the Formation of Vacuolar Inclusions

Phenolics and Ascorbic Acid Related to Antioxidant Activity of MaoFruit Juice and Their Thermal Stability Study (Review Article)

Cryoconcentration of flavonoid extract for enhanced biophotovoltaics and pH sensitive thin films

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