Fluorescence spectroscopic characterization of salivary metabolites of oral cancer patients

Yuvaraj, M.; Kanniyappan, Udayakumar; Jayanth, Vadivel; Rao, Aruna Prakasa; Bharanidharan, Ganesan; Koteeswaran, Dornadula; Munusamy, Balu David; C, Murali Krishna; Ganesan, Singaravelu

doi:10.1016/j.jphotobiol.2013.11.006

Cited by 55 publications

(33 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of porphyrin is usually attributed to changes associated with malignant transformation, and wherein the level of porphyrin is usually elevated in the effected tissue [16]. In our study, the effect of porphyrin manifests in Fig.…”

Section: Discussionsupporting

confidence: 51%

Handheld multispectral fluorescence lifetime imaging system for in vivo applications

Cheng

Cuenca

Liu

et al. 2014

Biomed. Opt. Express

View full text Add to dashboard Cite

Abstract:There is an increasing interest in the application of fluorescence lifetime imaging (FLIM) for medical diagnosis. Central to the clinical translation of FLIM technology is the development of compact and highspeed clinically compatible systems. We present a handheld probe design consisting of a small maneuverable box fitted with a rigid endoscope, capable of continuous lifetime imaging at multiple emission bands simultaneously. The system was characterized using standard fluorescent dyes. The performance was then further demonstrated by imaging a hamster cheek pouch in vivo, and oral mucosa tissue both ex vivo and in vivo, all using safe and permissible exposure levels. Such a design can greatly facilitate the evaluation of FLIM for oral cancer imaging in vivo.

show abstract

Section: Discussionsupporting

confidence: 51%

Handheld multispectral fluorescence lifetime imaging system for in vivo applications

Cheng

Cuenca

Liu

et al. 2014

Biomed. Opt. Express

View full text Add to dashboard Cite

show abstract

“…Furthermore, the removal of iron from the haem molecule to leave behind fluorescent protoporphyrin IX [ 20 ] would result in an hyperchromic shift (increase in fluorescence intensity). Such changes in the fluorescence of salivary protoporphyrin IX at 405 nm excitation have recently been suggested to be a potential tool to aid in the diagnosis of oral cancer [ 21 ]. The fluorescence dynamics within bacterial cultures is further complicated by the presence of other non-fluorescent porphyrins (i.e.…”

Section: Introductionmentioning

confidence: 99%

A Preliminary Study of the Effects of pH upon Fluorescence in Suspensions of Prevotella intermedia

et al. 2016

View full text Add to dashboard Cite

The quantification of fluorescence in dental plaque is currently being developed as a diagnostic tool to help inform and improve oral health. The oral anaerobe Prevotella intermedia exhibits red fluorescence due to the accumulation of porphyrins. pH affects the fluorescence of abiotic preparations of porphyrins caused by changes in speciation between monomers, higher aggregates and dimers, but this phenomenon has not been demonstrated in bacteria. Fluorescence spectra were obtained from suspensions of P. intermedia that were adjusted to pHs commensurate with the range found within dental plaque. Two fluorescent motifs were identified; 410 nm excitation / 634 nm emission (peak A) and 398 nm excitation / 622 nm emission (peak B). A transition in the fluorescence spectra was observed from peak A to peak B with increasing pH which was also evident as culture age increased from 24 hours to 96 hours. In addition to these ‘blue-shifts’, the intensity of peak A increased with pH whilst decreasing with culture age from 24 to 96 hours. A bacterium’s relationship with the local physiochemical environment at the time of image capture may therefore affect the quantification of dental plaque fluorescence.

show abstract

“…Sugimoto et al reported on the metabolomics of saliva of oral, breast and pancreatic cancer using capillary electrophoresis mass spectrometry and reported that the concentration of leucine, isoleucine, tryptophan, valine, threonine, histidine, alanine and phenylalanine in patients with OSCC were elevated than in normal subjects. The elevated levels of these amino acids in saliva are probably because of the degradation of proteins present in saliva and bacterial metabolism . Hence, this provides base to conclude that the elevation in the level of histidine, proline and valine in saliva of oral premalignant and malignant conditions may be because of the degradation of salivary proteins.…”

Section: Discussionmentioning

confidence: 90%

“…Sugimoto et al have used saliva to identify the metabolomics in saliva of oral, breast and pancreatic cancer using capillary electrophoresis mass spectrometry . Yuvaraj et al have reported on the native fluorescence of porphyrin elevation in oral cancer saliva . In the case of Raman spectroscopy, Kho et al and Li et al have reported on surface‐enhanced Raman scattering study of saliva in the detection of oral cancer and lung cancer using He–Ne laser excitation (633 nm) .…”

Section: Discussionmentioning

confidence: 99%

Near‐infrared Raman spectroscopic characterization of salivary metabolites in the discrimination of normal from oral premalignant and malignant conditions

Pachaiappan

Aruna

Brindha

et al. 2016

J Raman Spectroscopy

Self Cite

View full text Add to dashboard Cite

In the present study, Raman spectroscopy has been employed in the discrimination of the saliva of normal subjects from patients with oral submucous fibrosis and oral squamous cell carcinomaat 785-nm excitation. From the spectral signatures, prominent difference between normal and abnormal group because of variations in metabolic and pathological conditions of the subjects was observed. Principal component analysis coupled with linear discriminant analysis yielded a diagnostic sensitivity of 96.4 and 93.8% and a specificity of 70.2 and 95.7% in the classification of normal from premalignant and normal from malignant, respectively, confirming the efficacy of Raman spectroscopy in the classification of normal and oral abnormalities.

show abstract

Fluorescence spectroscopic characterization of salivary metabolites of oral cancer patients

Cited by 55 publications

References 28 publications

Handheld multispectral fluorescence lifetime imaging system for in vivo applications

Handheld multispectral fluorescence lifetime imaging system for in vivo applications

A Preliminary Study of the Effects of pH upon Fluorescence in Suspensions of Prevotella intermedia

Near‐infrared Raman spectroscopic characterization of salivary metabolites in the discrimination of normal from oral premalignant and malignant conditions

Contact Info

Product

Resources

About