Fluorescence Spectroscopy as a Tool for the Assessment of Liver Samples with Several Stages of Fibrosis

Fabila-Bustos, Diego A.; Arroyo-Camarena, Úrsula D.; López-Vancell, M. D.; Durán-Padilla, Marco A; Azuceno-García, Itzel; Stolik, S.; Valor, A.; Ibarra-Coronado, Elizabeth; Hernandez-Quintanar, L.; Escobedo, Galileo; Rosa, J. de la

doi:10.1089/pho.2017.4301

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…Nevertheless, a lesser invasive application of this tool in vivo is possible using endoscopic instrumentation, we have previously reported the use of DRS as an effective tool to evaluate the optical differences among normal and fibrotic liver both in an animal model of hepatic chronic disease 12 and in paraffin preserved human samples. 31 Other reports support DRS use in assessing liver pathologies. [14][15][16][17]24,32 NAFLD is an increasingly common hepatic disease worldwide, with a spectrum that comprises from simple steatosis, a benign lipid accumulation in the hepatic parenchyma; through NASH, which besides steatosis, includes inflammation and features of oxidative stress.…”

Section: Discussionmentioning

confidence: 98%

Diffuse reflectance spectroscopy accurately discriminates early and advanced grades of fatty liver in mice

et al. 2018

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Nonalcoholic fatty liver disease (NAFLD) ranges from steatosis to nonalcoholic steatohepatitis and cirrhosis. Liver biopsy, considered the gold standard to diagnose NAFLD, shows significantly high rates of interobserver variability. Thus there is a need to develop tools that accurately categorize mild and advanced grades of steatosis in order to identify patients at higher risk of developing chronic liver disease. Diffuse reflectance spectroscopy (DRS) has proved to be useful in grading liver fibrosis and cirrhosis, without having been implemented for steatosis. We aim to categorize early and advanced stages of liver steatosis in a methionine-choline deficient (MCD) mouse model. C57bl/6 mice are fed either methionine-choline control or MCD diet during 2 or 8 weeks to induce mild and advanced steatosis. Liver samples are obtained and steatosis is evaluated by oil red O staining. Diffuse reflectance spectra are directly measured on ex vivo liver specimens, in a wavelength range of 400 to 800 nm. DRS is able to discriminate between early or advanced steatosis and healthy hepatic tissue with negligible error while showing high average sensitivity and specificity (0.94 and 0.95, respectively). Our results suggest that liver steatosis can be accurately evaluated by DRS, highlighting the importance of applied spectroscopic methods in assessing NAFLD.

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Section: Discussionmentioning

confidence: 98%

Diffuse reflectance spectroscopy accurately discriminates early and advanced grades of fatty liver in mice

et al. 2018

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“…Correlation studies have been successfully employed previously either to inspect the applicability of the technique to be tested by comparing it with reference method or to measure the relationship between 2 variables . Fabila‐Bustos et al have recently utilized fluorescence spectroscopy as an analytical tool to discriminate different stages of fibrosis using liver tissue samples. Furthermore, they have reported a correlation between the shape of the fluorescence spectrum and level of liver fibrosis, and concluded that fluorescence spectroscopy could serve as a complimentary tool for clinical assessment of fibrosis.…”

Section: Discussionmentioning

confidence: 99%

Probing endogenous collagen by laser‐induced autofluorescence in burn wound biopsies: A pilot study

Prabhu

Acharya

Rao

et al. 2018

Journal of Biophotonics

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The focus of the current study was to interrogate the predictive potential of laser-induced autofluorescence (LIAF) by objectively assessing collagen synthesis in burn wound granulation tissues ex vivo. Prior grafting, granulation tissues (20 samples) following burn injury were collected from 17 subjects of age range 18 to 60 years with patient/donor consent and the corresponding autofluorescence spectra were recorded at 325 nm He-Cd laser (≈2 mW) excitations. The resulting endogenous collagen intensity from the above tissue samples was computed by normalizing the nicotinamide adenine dinucleotide levels. In addition, the hydroxyproline content was also estimated biochemically from the same granulation tissues. A comparative assessment of both LIAF and biochemical estimations for endogenous collagen by hydroxyproline resulted in strong positive correlation among them. The above relevant observations suggest that LIAF is equally informative as that of biochemical estimations, in evaluating endogenous collagen content in wound granulation tissues. Thus, it can be concluded that LIAF has the predictive potential, as a noninvasive objective tool to measure the endogenous collagen levels in wound biopsy tissues and provide complementary data conducive for making clinical decisions.

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Fluorescence spectroscopy on paraffin-preserved human liver samples to classify several grades of fibrosis

Roa-Tort

Rivera-Fernández

Rosa

et al. 2020

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Fluorescence Spectroscopy as a Tool for the Assessment of Liver Samples with Several Stages of Fibrosis

Abstract: With this study, it is concluded that fluorescence spectroscopy can be used as a complementary tool for the assessment of liver fibrosis in liver tissue samples, which sets the stage for subsequent clinical trials.

Cited by 4 publications

References 23 publications

Diffuse reflectance spectroscopy accurately discriminates early and advanced grades of fatty liver in mice

Diffuse reflectance spectroscopy accurately discriminates early and advanced grades of fatty liver in mice

Probing endogenous collagen by laser‐induced autofluorescence in burn wound biopsies: A pilot study

Fluorescence spectroscopy on paraffin-preserved human liver samples to classify several grades of fibrosis

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