Fluorescence study on the interaction of human serum albumin with loureirin B

Abstract

Abstract. The interaction between loureirin B (Lour B) and human serum albumin (HSA) was investigated by fluorescence and UV-vis absorption spectroscopy. Experimental results indicated that loureirin B had a strong ability to quench the intrinsic fluorescence of HSA through a dynamic quenching procedure. The fluorescence quenching data revealed that the quenching constants (K SV ) 2.68 × 10 4 , 3.30 × 10 4 and 4.10 × 10 4 l/mol at 300, 310 and 320 K, respectively. Based on the thermodynamic parameters obtained, the positive values of enthalpy change ΔH and entropy change ΔS suggested that hydrophobic forces played a major role in the interaction of Lour B with HSA. According to Förster theory of energy transfer, the distance r between HSA and Lour B was calculated to be 2.85 nm. Furthermore, the effect of Lour B on the conformation of HSA was analyzed by synchronous fluorescence and three-dimensional fluorescence spectra.

β-Lactoglobulin as a Nanotransporter for Glabridin: Exploring the Binding Properties and Bioactivity Influences

“…Therefore, the migration of the wavelength can be used as a basis for judging whether the conformation of the protein changes. 29 Figure 2 shows the fluorescence emission spectra of β-lg in the presence of different concentrations of glabridin. The intensity of the fluorescence emission of β-lg at around 325 nm gradually decreased and upshifted to 334 nm with an increase in the glabridin concentration.…”

“…Furthermore, the fluorescence intensity of any fluorescent substance is reduced in the presence of a fluorescent quencher such as glabridin. Therefore, the migration of the wavelength can be used as a basis for judging whether the conformation of the protein changes Figure shows the fluorescence emission spectra of β-lg in the presence of different concentrations of glabridin.…”

Online Spectroscopic Study on the Positive and the Negative Electrolytes in Vanadium Redox Flow Batteries

“…e technique of spectroscopy has been widely used in chemical composition analysis and the study of chemical reaction dynamics [1,2]. Traditional spectroscopic analysis is based on the Beer-Lambert law and has two requirements:…”

“…(1) the concentration of the analyte is low; (2) there is no interference between different compositions of the analyte. However, analytes in many chemical and chemical engineering applications do not meet these two requirements and hence cannot be analyzed by the traditional Beer-Lambert law-based spectroscopic analysis.…”