Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review

Steingart, Karen R; Henry, Marie‐Claire; Ng, Vivienne; Hopewell, Philip C.; Ramsay, Andrew; Cunningham, Jane; Urbanczik, R; Perkins, Mark D.; Aziz, Mohamed Abdel; Pai, Madhukar

doi:10.1016/s1473-3099(06)70578-3

Cited by 664 publications

(487 citation statements)

References 23 publications

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“…For the patients who cannot take out sputum, sputum induction, bronchoscopy, or gastric juice collection must be considered. The sensitivity of standard microscopy can be increased by 10% with fluorescent microscopy (12). Another advantage of fluorescent microscopy is that samples can be evaluated faster.…”

Section: A Microbiologymentioning

confidence: 99%

Smear-Negative Pulmonary Tuberculosis

Çalışkan¹,

Kaya²

2015

Eurasian J Pulmonol

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Tuberculosis (TB) is the second leading cause of death from an infectious disease worldwide, after the human immunodeficiency virus. There are almost 2.5 million new smear-positive pulmonary TB cases and 1.9 million new smear-negative pulmonary TB cases in the world in 2012 according to the World Health Organization (WHO) Global Tuberculosis Report 2013. Smear negativity in pulmonary TB is a common clinical problem. Clinicians have difficulty in diagnosing smear-negative pulmonary TB without bacteriological confirmation. It is very important to decide whether or not to treat a patient with smear negative pulmonary TB when the culture results are pending or negative. New diagnostic methods are required for the diagnosis of smear-negative pulmonary TB. In addition to the development of new microbiological and serological diagnostic tests, clinical prediction scoring systems and algorithms including clinical and radiological findings of smear-negative pulmonary TB patients in our country, should be established to facilitate the diagnosis of smear-negative pulmonary TB.

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Section: A Microbiologymentioning

confidence: 99%

Smear-Negative Pulmonary Tuberculosis

Çalışkan¹,

Kaya²

2015

Eurasian J Pulmonol

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“…A widely cited reason for the ongoing gap between incidence and cases notified is the lack of highly sensitive and deployable diagnostic tests for TB 3 . Sputum smear microscopy, the global cornerstone of TB diagnosis 4 , can miss half of all people with infectious TB 5 , whereas more sensitive tests cannot routinely be implemented at the point of treatment 6,7 . Nevertheless, the link between improved diagnostic sensitivity and better TB detection remains uncertain.…”

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confidence: 99%

Understanding the incremental value of novel diagnostic tests for tuberculosis

Arinaminpathy

Dowdy

2015

Nature

109

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Tuberculosis is a major source of global mortality caused by infection, partly because of a tremendous ongoing burden of undiagnosed disease. Improved diagnostic technology may play an increasingly crucial part in global efforts to end tuberculosis, but the ability of diagnostic tests to curb tuberculosis transmission is dependent on multiple factors, including the time taken by a patient to seek health care, the patient's symptoms, and the patterns of transmission before diagnosis. Novel diagnostic assays for tuberculosis have conventionally been evaluated on the basis of characteristics such as sensitivity and specificity, using assumptions that probably overestimate the impact of diagnostic tests on transmission. We argue for a shift in focus to the evaluation of such tests' incremental value, defining outcomes that reflect each test's purpose (for example, transmissions averted) and comparing systems with the test against those without, in terms of those outcomes. Incremental value can also be measured in units of outcome per incremental unit of resource (for example, money or human capacity). Using a novel, simplified model of tuberculosis transmission that addresses some of the limitations of earlier tuberculosis diagnostic models, we demonstrate that the incremental value of any novel test depends not just on its accuracy, but also on elements such as patient behaviour, tuberculosis natural history and health systems. By integrating these factors into a single unified framework, we advance an approach to the evaluation of new diagnostic tests for tuberculosis that considers the incremental value at the population level and demonstrates how additional data could inform more-effective implementation of tuberculosis diagnostic tests under various conditions

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“…An alternative for the light microscope is the fluorescent microscope. In this technique, the fluorescent bacilli of M. tuberculosis can be seen at lower magnification and the smears can be examined in less time [16]. However the implementation of this technique was difficult due to higher cost associated with purchase of the microscope with a mercury vapor lamp.…”

Section: Introduction:-mentioning

confidence: 99%