Fluorescent and colorimetric assay for determination of Cu(II) and Hg(II) using AuNPs reduced and wrapped by carbon dots

Li, Yuxi; Tang, Lu; Zhu, Chenxue; Liu, Xunyong; Wang, Xing; Liu, Yi

doi:10.1007/s00604-021-05111-6

Cited by 36 publications

(15 citation statements)

References 34 publications

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“…In the presence \ of hydrogen peroxide (H 2 O 2 ), the fluorescent substrate is catalyzed with peroxidase-like properties. In order to make the sensor more stable to heat, pH, and salt, Li [47] et al designed a fluorescent and colorimetric dual-modal sensor based on AuNPs prepared using carbon dots (CDs) to detect the concentration of Cu 2+ and Hg 2+ in water. In addition to environmental heavy metal detection, AuNPs can also be used to analyze cancer biomarkers through dual-mode fluorescence colorimetry.…”

Section: Fluorescent and Colorimetric Assaymentioning

confidence: 99%

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Chan

Chang

et al. 2023

Molecules

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Molecular biology applications based on gold nanotechnology have revolutionary impacts, especially in diagnosing and treating molecular and cellular levels. The combination of plasmonic resonance, biochemistry, and optoelectronic engineering has increased the detection of molecules and the possibility of atoms. These advantages have brought medical research to the cellular level for application potential. Many research groups are working towards this. The superior analytical properties of gold nanoparticles can not only be used as an effective drug screening instrument for gene sequencing in new drug development but also as an essential tool for detecting physiological functions, such as blood glucose, antigen-antibody analysis, etc. The review introduces the principles of biomedical sensing systems, the principles of nanomaterial analysis applied to biomedicine at home and abroad, and the chemical surface modification of various gold nanoparticles.

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Section: Fluorescent and Colorimetric Assaymentioning

confidence: 99%

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Chan

Chang

et al. 2023

Molecules

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“…30−35 For example, Li et al designed a colorimetric and fluorescent dual-mode sensor for the determination of Hg 2+ to improve the accuracy of the sensor. 29 However, till now, there is no work on the dual-mode assay of Hg 2+ based on the photocurrent-polarity-switching PEC method. Additionally, colorimetry is a popular method in the analytical field owing to the cost-effectiveness, visualization with naked eye, and simplicity.…”

Section: ■ Introductionmentioning

confidence: 99%

“…As we all know, the assay results from single-mode signal readout will be affected by operators, apparatus, and nonstandard analytical procedures. , In order to improve the accuracy and confidence of the assay, recently, dual-mode sensing methods have received wide attention due to the relatively independent signal transductions and different response mechanisms in different modes. This makes the dual-mode sensing platform not only have distinct properties of each mode but also can provide mutual authentication of detection results. − For example, Li et al designed a colorimetric and fluorescent dual-mode sensor for the determination of Hg 2+ to improve the accuracy of the sensor . However, till now, there is no work on the dual-mode assay of Hg 2+ based on the photocurrent-polarity-switching PEC method.…”

Section: Introductionmentioning

confidence: 99%

“…As we all know, the assay results from single-mode signal readout will be affected by operators, apparatus, and nonstandard analytical procedures. 28,29 In order to improve the accuracy and confidence of the assay, recently, dual-mode sensing methods have received wide attention due to the relatively independent signal transductions and different response mechanisms in different modes. This makes the dual-mode sensing platform not only have distinct properties of each mode but also can provide mutual authentication of detection results.…”

Section: ■ Introductionmentioning

confidence: 99%

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Colorimetric and Photocurrent-Polarity-Switching Photoelectrochemical Dual-Mode Sensing Platform for Highly Selective Detection of Mercury Ions Based on the Split G-Quadruplex–Hemin Complex

Zhang

et al. 2022

Anal. Chem.

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Mercury ion (Hg2+) is one of the most harmful heavy metal ions with the greatest impact on public health. Herein, based on the excellent catalytic activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) and the strong photocurrent-polarity-switching ability to SnS2 photoanode of the split G-quadruplex–hemin complex, the magnetic NiCo2O4@SiO2–NH2 sphere-assisted colorimetric and photoelectrochemical (PEC) dual-mode sensing platform was developed for the Hg2+ assay. First, the amino-labelled single-stranded DNA1 (S1) was immobilized on NiCo2O4@SiO2–NH2 and then partly hybridized with another single-stranded DNA2 (S2). When Hg2+ was present, the thymine–Hg2+–thymine base pairs between S1 and S2 were formed, causing the formation of the split G-quadruplex in the presence of K+. After addition of hemin, the split G-quadruplex–hemin complex was obtained and effectually catalyzed the H2O2-mediated oxidation of TMB. Thus, the color and absorbance intensity of the TMB solution were changed, resulting in the visual and colorimetric detection of Hg2+. The linear response range is 10 pM to 10 nM, and the detection limit is 3.8 pM. Meanwhile, the above G-quadruplex–hemin complex effectively switched the photocurrent polarity of SnS2-modified indium tin oxide electrode, leading to the sensitive and selective PEC assay of Hg2+ with a linear response range of 5 pM to 500 nM and a detection limit of 2.3 pM. Moreover, the developed dual-mode sensing platform provided mutual authentication of detection results in different modes, effectively improving the assay accuracy and confidence, and may have a good potential application in highly sensitive, selective, and accurate determination of Hg2+ in environmental fields.

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“…Unlike other signals (such as electrochemical, magnetic, Raman, and thermal signals), the color/fluorescence dual-modal signals can share a similar optical readout device, thus facilitating data collection and further exhibiting great potential in developing low-cost, point-of-need detection methods. Although some scholars have investigated the combination of AuNPs and fluorescent materials in multimodal bioassays, − there is still ample room for progress, especially in the improvement of sensitivity, accuracy, and the simplification of probe synthesis. In these reported AuNPs-based fluorescent detection probes, only a single AuNP was employed to couple with fluorescent dyes or fluorescent nanomaterials (carbon dots and upconversion nanoparticles).…”

Section: Introductionmentioning

confidence: 99%

Facile Fabrication of Highly Quantum Dot/AuNP-Loaded Tags for a Dual-Modal Colorimetric/Reversed Ratiometric Fluorescence Immunochromatographic Assay

et al. 2022

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Developing an easily-prepared, sensitive, and accurate point-of-need immunochromatographic assay (ICA) is significant in food safety screening, clinical diagnosis, and environmental monitoring. However, the current single-modal ICAs are limited in certain instinct drawbacks that restrict analytical performances. Herein, we introduce an ultrasensitive dual-modal colorimetric/reversed ratiometric fluorescence ICA based on facilely prepared immunoprobes with a high loading capacity of red quantum dots and AuNPs. By smartly integrating these red-colored/fluorescent signal probes with an immobilized green quantum dot antigen on the test lines, discrete “turn-on” visual inspection and reversed ratiometric quantification via a portable smartphone-based analyzer were accomplished. As an application, this method was employed to detect 11 phosphodiesterase-5 inhibitors in health foods with ultralow detection limits (0.0028–0.045 ng/mL), high repeatability (coefficient of variations of 0.3–1.91%), and reasonable accuracy (recoveries of 86.6–107%). The proposed method was further validated by the authorized liquid chromatography with tandem mass spectrometry method in actual sample detection. This new assay format can be extended to ultrasensitive flexible detection of other food contaminants, environmental pollutants, or tumor biomarkers within minutes, and it just requires simply prepared signal reporters, easy-to-operate procedures, and a low-cost miniaturized analyzer.

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Fluorescent and colorimetric assay for determination of Cu(II) and Hg(II) using AuNPs reduced and wrapped by carbon dots

Cited by 36 publications

References 34 publications

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Gold Nanoparticles as a Biosensor for Cancer Biomarker Determination

Colorimetric and Photocurrent-Polarity-Switching Photoelectrochemical Dual-Mode Sensing Platform for Highly Selective Detection of Mercury Ions Based on the Split G-Quadruplex–Hemin Complex

Facile Fabrication of Highly Quantum Dot/AuNP-Loaded Tags for a Dual-Modal Colorimetric/Reversed Ratiometric Fluorescence Immunochromatographic Assay

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