Fluorescent Biosensors for Protein Interactions and Drug Discovery

Sosa‐Peinado, Alejandro; González-Andrade, Martín

doi:10.5772/17431

Cited by 2 publications

(5 citation statements)

References 36 publications

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“…In general, the fluorescent attachment is based in attachment of chemical groups and/or in the genetic fusion of green fluorescent proteins (GFP) or derived proteins. However, the application of labels through chemical methods have the advantage of introducing the label at any position [120]. Regarding the application of enzymes as recognition element, as the catalysis is not desirable in some cases, some enzymes have been modified to impair their activity and conserve only the ligand binding property [120].…”

Section: Biosensosr Based On Transductionmentioning

confidence: 99%

“…However, the application of labels through chemical methods have the advantage of introducing the label at any position [120]. Regarding the application of enzymes as recognition element, as the catalysis is not desirable in some cases, some enzymes have been modified to impair their activity and conserve only the ligand binding property [120]. Also, Ab-based fluorescent biosensors are beginning to receive attention as suitable biomolecules for diagnostics in homogeneous immunoassays or as imaging probes [121].…”

Section: Biosensosr Based On Transductionmentioning

confidence: 99%

“…Calmoduilin (CaM) is a calcium binding proteins that represents an important drug target for many drugs including antipsychotic, smooth muscle relaxants, antitumoral and α-adrenergic blocking agents, among others [120]. Therefore, CaM has been used as biological recognition element for the design of fluorescent biosensors in order to study interactions between this and other molecules, for example, the interaction between the CaM coupled to three different fluorophores and phenothiazines and related tryclic antidepressants [120]. Recently, a new fluorescent biosensor based on a human CaM (hCaM) was constructed for the detection of calcium ion (Ca 2+ ) and CaM inhibitors in solution.…”

Section: Biosensosr Based On Transductionmentioning

confidence: 99%

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Trends in Protein-Based Biosensor Assemblies for Drug Screening and Pharmaceutical Kinetic Studies

et al. 2014

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Abstract:The selection of natural and chemical compounds for potential applications in new pharmaceutical formulations constitutes a time-consuming procedure in drug screening. To overcome this issue, new devices called biosensors, have already demonstrated their versatility and capacity for routine clinical diagnosis. Designed to perform analytical analysis for the detection of a particular analyte, biosensors based on the coupling of proteins to amperometric and optical devices have shown the appropriate selectivity, sensibility and accuracy. During the last years, the exponential demand for pharmacokinetic studies in the early phases of drug development, along with the need of lower molecular weight detection, have led to new biosensor structure materials with innovative immobilization strategies. The result has been the development of smaller, more reproducible biosensors with lower detection limits, and with a drastic reduction in the required sample volumes. Therefore in order to describe the main achievements in biosensor fields, the present review has the main aim of summarizing the essential strategies used to generate these specific devices, that can provide, under physiological conditions, a credible molecule profile and assess specific pharmacokinetic parameters.

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Section: Biosensosr Based On Transductionmentioning

confidence: 99%

Section: Biosensosr Based On Transductionmentioning

confidence: 99%

Section: Biosensosr Based On Transductionmentioning

confidence: 99%

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Trends in Protein-Based Biosensor Assemblies for Drug Screening and Pharmaceutical Kinetic Studies

et al. 2014

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“…The principle of their work is based on the quencher/ligand or the OFF state, where fluorescence occurs only due to binding of molecule of interest. In that case, the quencher is removed and detection signal is exhibited (Figure 3) [44]. …”

Section: The Quencher/ligand Systemmentioning

confidence: 99%

“…As transducer that must be attached to the protein, the fluorescent proteins have been used [44,45].…”

Section: The Quencher/ligand Systemmentioning

confidence: 99%

Perspective potential of polymer-based biosensor chips in food industry and clinical diagnostics

Mehmedinovi¹,

Ibriimovi²,

Kesi³

et al. 2017

Biodegradable Polymers: Recent Developments and New Perspectives

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Fluorescent Biosensors for Protein Interactions and Drug Discovery

Cited by 2 publications

References 36 publications

Trends in Protein-Based Biosensor Assemblies for Drug Screening and Pharmaceutical Kinetic Studies

Trends in Protein-Based Biosensor Assemblies for Drug Screening and Pharmaceutical Kinetic Studies

Perspective potential of polymer-based biosensor chips in food industry and clinical diagnostics

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