Fluorescent coelenteramide-containing protein as a color bioindicator for low-dose radiation effects

Petrova, Alena S.; Lukonina, Anna A.; Badun, G. A.; Kudryasheva, Nadezhda S.

doi:10.1007/s00216-017-0404-9

Cited by 9 publications

(15 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tritium bioeffects were studied using the simplest enzymatic process, i.e., the photobiochemical reaction of Coelinteramide-containing Fluorescent Protein (CLM-FP). Previously, the low-dose exposure of CLM-FP to tritium was studied by Petrova et al [49]. No activation was found, but only an inhibition effect of tritium: the 18-day chronic exposure to HTO resulted in an increase in the violet fluorescence component contribution.…”

Section: Effects Of Tritium On Coelinteramide-containing Fluorescent mentioning

confidence: 95%

“…CLM-FPs are products of bioluminescent reactions of coelenterates that constitute a potential use in biomedicine as biomarkers [36][37][38]. The fluorescence characteristics of CLM-FPs were intensively studied [39][40][41][42][43][44][45][46]; they were found to be sensitive to external exposures: excitation energy [44], chemical agents [47,48], and radioactivity [49,50]. Recently, [51] suggested CLM-FPs for intracellular application as being the simplest toxicity bioassay.…”

Section: ⎯⎯mentioning

confidence: 99%

“…The detailed methodology of the experiment was described in [49]. HTO was added to the CLM-FP solutions.…”

Section: Effect Of Tritium On Photoluminescence Of Clm-fp Reagents Pmentioning

confidence: 99%

See 2 more Smart Citations

Enzymatic Responses to Low-Intensity Radiation of Tritium

Rozhko

Nemtseva

Gardt

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

The present study considers a possible role of enzymatic reactions in the adaptive response of cells to the beta-emitting radionuclide tritium under conditions of low-dose exposures. Effects of tritiated water (HTO) on the reactions of bacterial luciferase and NAD(P)H:FMN-oxidoreductase, as well as a coupled system of these two reactions, were studied at radioactivity concentrations ≤ 200 MBq/L. Additionally, one of the simplest enzymatic reactions, photobiochemical proton transfer in Coelenteramide-containing Fluorescent Protein (CLM-FP), was also investigated. We found that HTO increased the activity of NAD(P)H:FMN-oxidoreductase at the initial stage of its reaction (by up to 230%); however, a rise of luciferase activity was moderate (<20%). The CLM-FP samples did not show any increase in the rate of the photobiochemical proton transfer under the exposure to HTO. The responses of the enzyme systems were compared to the ‘hormetic’ response of luminous marine bacterial cells studied earlier. We conclude that (1) the oxidoreductase reaction contributes significantly to the activation of the coupled enzyme system and bacterial cells by tritium, and (2) an increase in the organization level of biological systems promotes the hormesis phenomenon.

show abstract

Section: Effects Of Tritium On Coelinteramide-containing Fluorescent mentioning

confidence: 95%

Section: ⎯⎯mentioning

confidence: 99%

See 1 more Smart Citation

Enzymatic Responses to Low-Intensity Radiation of Tritium

Rozhko

Nemtseva

Gardt

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…20 In addition, in some experimental biosystems using lower organisms, application of hydrozoan Obelia longissima harboring bioluminescence reaction and introduction of GFP into bacteria Deinococcus radiodurans were subjected to evaluation on the biological effects of chronic low-dose beta radiation from tritiated water and in situ real-time evaluation of radiationresponsive promoters. 21,22 GFP of jellyfish Aequorea victoria is an unusual protein with visible absorbance and fluorescence. Unlike other reporters, GFP fluorescence emerges in the absence of substrates or cofactors due to that GFP self-contains a fluorescent p-hydroxybenzylidene-imidazolidinone chromophore in the peptide chains.…”

Section: Introductionmentioning

confidence: 99%

Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice

et al. 2020

View full text Add to dashboard Cite

Application of green fluorescent protein (GFP) in a variety of biosystems as a unique bioindicator or biomarker has revolutionized biological research and made groundbreaking achievements, while increasing evidence has shown alterations in biological properties and physiological functions of the cells and animals overexpressing transgenic GFP. In this work, response to total body irradiation (TBI) was comparatively studied in GFP transgenic C57BL/6-Tg (CAG-EGFP) mice and C57BL/6 N wild type mice. It was demonstrated that GFP transgenic mice were more sensitive to radiation-induced bone marrow death, and no adaptive response could be induced. In the nucleated bone marrow cells of GFP transgenic mice exposed to a middle dose, there was a significant increase in both the percentage of cells expressing pro-apoptotic gene Bax and apoptotic cell death. While in wild type cells, lower expression of pro-apoptotic gene Bax and higher expression of anti-apoptotic gene Bcl-2, and significant lower induction of apoptosis were observed compared to GFP transgenic cells. Results suggest that presence of GFP could alter response to TBI at whole body, cellular and molecular levels in mice. These findings indicate that there could be a major influence on the interpretation of the results obtained in GFP transgenic mice.

show abstract

“…Relative contributions of spectral components to fluorescence spectra of CLM-containing protein ("discharged obelin" from Obelia longissima) exposed to (A) tritiated water, 200 MBq/L[73];(B) gamma radiation,137 Cs, 2 mGy/h, 20°С[74].Dependence of the response of CLM-containing protein to low-intensive gamma radiation on temperature was demonstrated in[74] as well.Hence, simplest bioassay systems (based on enzymatic reaction and physicochemical processes in protein complex) show lower ability for adaptive response to low-dose radioactive exposures. In contrast to bacterial cells, they can demonstrate dependence on intensity of irradiation or absence of the activation stage in the course of chronic exposure.…”

mentioning

confidence: 97%

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

Kudryasheva¹,

Kovel²

2019

Preprint

Self Cite

View full text Add to dashboard Cite

Current paper reviews applications of luminescence bioassays for monitoring low-intensity factors, namely, radioactivity of different types (alpha, beta and gamma), and bioactive compounds (humic substances and fullerenols). Luminescence intensity is taken as a physiological parameter of luminous organisms. High rates of luminescence response can provide (1) a proper number of experiments under comparable conditions and, therefore, proper statistical processing, with this being highly important for ‘noisy’ low-dose exposures; (2) non-genetic, i.e. biochemical and physicochemical mechanisms of cellular response, in accordance to “exposome” concept. Bioassays based on luminous marine bacteria, their enzymes, and fluorescence coelenteramide-containing proteins were used to compare results of low-intensity exposures at cellular, biochemical and physicochemical levels, respectively. Results of the cellular exposures were discussed in terms of hormesis concept. Bioluminescence time dependence under low-dose radiation exposures corresponded to hormesis or threshold models; no bioluminescence monotonic dependency on intensity of exposure (dose rate, radioactivity, concentration) was found. Bioluminescence activation and absence of its dependency on intensity of exposure can be accepted as features of cellular adaptive response. Changes of biological luminescence were analyzed and discussed for bioassays of lower organization level – enzymes and florescent protein.

show abstract

Fluorescent coelenteramide-containing protein as a color bioindicator for low-dose radiation effects

Cited by 9 publications

References 20 publications

Enzymatic Responses to Low-Intensity Radiation of Tritium

Enzymatic Responses to Low-Intensity Radiation of Tritium

Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

Contact Info

Product

Resources

About